MCA_01192_1

Protein

Length

625 amino acids

Gene name: RFA1

Description: Replication factor A protein 1

Browser: contigA:3776436-3778314-

RNA-seq: read pairs 1806, FPKM 35.6, percentile rank 57.5% (100% = highest expression)

Protein function

Annotation:		RFA1	Replication factor A protein 1
KEGG:	K07466	RFA1	replication factor A1
EGGNOG:	0PGI7	RFA1	replication
SGD closest match:	S000000065	RFA1	Replication factor A protein 1
CGD closest match:	CAL0000199773	RFA1	Replication factor A subunit protein

Protein alignments

	%id	Aln length	E-value
MIA_06286_1	40.17%	600	4e-137	MIA_06286_1
A0A167C4L4_9ASCO	41.23%	439	2e-116	Replication factor A subunit protein RFA1 OS=Sugiyamaella lignohabitans GN=RFA1 PE=4 SV=1
UniRef50_A0A167C4L4	41.23%	439	5e-113	Replication factor A subunit protein RFA1 n=1 Tax=Sugiyamaella lignohabitans TaxID=796027 RepID=A0A167C4L4_9ASCO
A0A0J9X3U7_GEOCN	42.40%	441	6e-107	Similar to Saccharomyces cerevisiae YAR007C RFA1 Subunit of heterotrimeric Replication Protein A (RPA) OS=Geotrichum candidum GN=BN980_GECA01s05851g PE=4 SV=1
RFA1_YEAST	37.01%	462	1e-101	Replication factor A protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RFA1 PE=1 SV=1
Q6C5S2_YARLI	33.39%	632	1e-99	YALI0E15642p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E15642g PE=4 SV=1
A0A1E3PN68_9ASCO	37.50%	448	4e-96	Replication factor-a protein (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_10216 PE=4 SV=1
Q5ACX4_CANAL	30.84%	616	4e-92	Replication factor A subunit protein OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=RFA1 PE=4 SV=1
A0A060TDG7_BLAAD	35.18%	469	1e-84	ARAD1D43714p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D43714g PE=4 SV=1
A0A1E4TAA8_9ASCO	30.82%	438	3e-59	Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_15260 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.2953

Protein family membership

Replication factor A protein 1 (IPR004591)

Domains and repeats

Domain
190 - 301
Nucleic acid-binding, OB-fold (IPR012340)
310 - 430
Nucleic acid-binding, OB-fold (IPR012340)
454 - 625
Nucleic acid-binding, OB-fold (IPR012340)
Domain
316 - 411
Replication protein A, OB domain (IPR031657)
459 - 624
Replication factor A, C-terminal (IPR013955)

1 100 200 300 400 500 625

Detailed signature matches

IPR004591

Replication factor A protein 1
1. TIGR00617
  
  9 - 623
  
  TIGRFAMs
  TIGR00617 (TIGR00617)
IPR012340

Nucleic acid-binding, OB-fold
1. SSF50249 (Nucleic a...)
  
  190 - 301
  
  SUPERFAMILY
  SSF50249 (Nucleic acid-binding proteins)
  
  454 - 625
  
  SUPERFAMILY
  SSF50249 (Nucleic acid-binding proteins)
  
  310 - 430
  
  SUPERFAMILY
  SSF50249 (Nucleic acid-binding proteins)
IPR031657

Replication protein A, OB domain
1. PF16900 (REPA_OB_2)
  
  316 - 411
  
  Pfam
  PF16900 (REPA_OB_2)
IPR013955

Replication factor A, C-terminal
1. cd04476 (RPA1_DBD_C)
  
  459 - 624
  
  CDD
  cd04476 (RPA1_DBD_C)
2. PF08646 (Rep_fac-A_C)
  
  473 - 616
  
  Pfam
  PF08646 (Rep_fac-A_C)

no IPR

Unintegrated signatures

Coil

138 - 162

COILS
Coil (Coil)
G3DSA:1.10.11...

23 - 193

GENE3D
G3DSA:1.10.110.10 (G3DSA:1.10.110.10)
G3DSA:2.40.50...

288 - 476

GENE3D
G3DSA:2.40.50.140 (G3DSA:2.40.50.140)

486 - 625

GENE3D
G3DSA:2.40.50.140 (G3DSA:2.40.50.140)

194 - 287

GENE3D
G3DSA:2.40.50.140 (G3DSA:2.40.50.140)
cd04474 (RPA1_DBD_A)

194 - 294

CDD
cd04474 (RPA1_DBD_A)
cd04475 (RPA1_DBD_B)

326 - 426

CDD
cd04475 (RPA1_DBD_B)
mobidb-lite (disord...)

142 - 189

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

generic binding su...

205V

generic binding surface II
205V, 261V, 263V, 265S, 294E
CDD
cd04474 (RPA1_DBD_A)

261V

generic binding surface II
205V, 261V, 263V, 265S, 294E
CDD
cd04474 (RPA1_DBD_A)

263V

generic binding surface II
205V, 261V, 263V, 265S, 294E
CDD
cd04474 (RPA1_DBD_A)

265S

generic binding surface II
205V, 261V, 263V, 265S, 294E
CDD
cd04474 (RPA1_DBD_A)

294E

generic binding surface II
205V, 261V, 263V, 265S, 294E
CDD
cd04474 (RPA1_DBD_A)
DBD-A/DBD-B interf...

212D

DBD-A/DBD-B interface
212D, 239E, 278N, 280N
CDD
cd04474 (RPA1_DBD_A)

239E

DBD-A/DBD-B interface
212D, 239E, 278N, 280N
CDD
cd04474 (RPA1_DBD_A)

278N

DBD-A/DBD-B interface
212D, 239E, 278N, 280N
CDD
cd04474 (RPA1_DBD_A)

280N

DBD-A/DBD-B interface
212D, 239E, 278N, 280N
CDD
cd04474 (RPA1_DBD_A)
ssDNA binding site...

217R

ssDNA binding site
217R, 241K, 243T, 245F, 248Q, 270T, 276F, 284E, 286M, 287L
CDD
cd04474 (RPA1_DBD_A)

241K

ssDNA binding site
217R, 241K, 243T, 245F, 248Q, 270T, 276F, 284E, 286M, 287L
CDD
cd04474 (RPA1_DBD_A)

243T

ssDNA binding site
217R, 241K, 243T, 245F, 248Q, 270T, 276F, 284E, 286M, 287L
CDD
cd04474 (RPA1_DBD_A)

245F

ssDNA binding site
217R, 241K, 243T, 245F, 248Q, 270T, 276F, 284E, 286M, 287L
CDD
cd04474 (RPA1_DBD_A)

248Q

ssDNA binding site
217R, 241K, 243T, 245F, 248Q, 270T, 276F, 284E, 286M, 287L
CDD
cd04474 (RPA1_DBD_A)

270T

ssDNA binding site
217R, 241K, 243T, 245F, 248Q, 270T, 276F, 284E, 286M, 287L
CDD
cd04474 (RPA1_DBD_A)

276F

ssDNA binding site
217R, 241K, 243T, 245F, 248Q, 270T, 276F, 284E, 286M, 287L
CDD
cd04474 (RPA1_DBD_A)

284E

ssDNA binding site
217R, 241K, 243T, 245F, 248Q, 270T, 276F, 284E, 286M, 287L
CDD
cd04474 (RPA1_DBD_A)

286M

ssDNA binding site
217R, 241K, 243T, 245F, 248Q, 270T, 276F, 284E, 286M, 287L
CDD
cd04474 (RPA1_DBD_A)

287L

ssDNA binding site
217R, 241K, 243T, 245F, 248Q, 270T, 276F, 284E, 286M, 287L
CDD
cd04474 (RPA1_DBD_A)
DBD-B/DBD-A interf...

328D

DBD-B/DBD-A interface
328D, 390K, 407S, 408G, 409Q
CDD
cd04475 (RPA1_DBD_B)

390K

DBD-B/DBD-A interface
328D, 390K, 407S, 408G, 409Q
CDD
cd04475 (RPA1_DBD_B)

407S

DBD-B/DBD-A interface
328D, 390K, 407S, 408G, 409Q
CDD
cd04475 (RPA1_DBD_B)

408G

DBD-B/DBD-A interface
328D, 390K, 407S, 408G, 409Q
CDD
cd04475 (RPA1_DBD_B)

409Q

DBD-B/DBD-A interface
328D, 390K, 407S, 408G, 409Q
CDD
cd04475 (RPA1_DBD_B)
ssDNA binding site...

368T

ssDNA binding site
368T, 370W, 393K, 397F, 403S, 407S
CDD
cd04475 (RPA1_DBD_B)

370W

ssDNA binding site
368T, 370W, 393K, 397F, 403S, 407S
CDD
cd04475 (RPA1_DBD_B)

393K

ssDNA binding site
368T, 370W, 393K, 397F, 403S, 407S
CDD
cd04475 (RPA1_DBD_B)

397F

ssDNA binding site
368T, 370W, 393K, 397F, 403S, 407S
CDD
cd04475 (RPA1_DBD_B)

403S

ssDNA binding site
368T, 370W, 393K, 397F, 403S, 407S
CDD
cd04475 (RPA1_DBD_B)

407S

ssDNA binding site
368T, 370W, 393K, 397F, 403S, 407S
CDD
cd04475 (RPA1_DBD_B)
generic binding su...

475F

generic binding surface II
475F, 581P, 583V, 585Q, 607S
CDD
cd04476 (RPA1_DBD_C)

581P

generic binding surface II
475F, 581P, 583V, 585Q, 607S
CDD
cd04476 (RPA1_DBD_C)

583V

generic binding surface II
475F, 581P, 583V, 585Q, 607S
CDD
cd04476 (RPA1_DBD_C)

585Q

generic binding surface II
475F, 581P, 583V, 585Q, 607S
CDD
cd04476 (RPA1_DBD_C)

607S

generic binding surface II
475F, 581P, 583V, 585Q, 607S
CDD
cd04476 (RPA1_DBD_C)
generic binding su...

480V

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

481C

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

482S

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

529S

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

530V

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

531A

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

532I

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

534D

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

540W

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

541V

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

542T

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

544F

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

580C

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

587R

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

588A

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

589K

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

600K

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

601Y

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)

602Q

generic binding surface I
480V, 481C, 482S, 529S, 530V, 531A, 532I, 534D, 540W, 541V, 542T, 544F, 580C, 587R, 588A, 589K, 600K, 601Y, 602Q
CDD
cd04476 (RPA1_DBD_C)
RPA1 DBD-C/RPA2 DB...

481C

RPA1 DBD-C/RPA2 DBD-D interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 618L, 622I
CDD
cd04476 (RPA1_DBD_C)

534D

RPA1 DBD-C/RPA2 DBD-D interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 618L, 622I
CDD
cd04476 (RPA1_DBD_C)

535E

RPA1 DBD-C/RPA2 DBD-D interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 618L, 622I
CDD
cd04476 (RPA1_DBD_C)

575S

RPA1 DBD-C/RPA2 DBD-D interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 618L, 622I
CDD
cd04476 (RPA1_DBD_C)

576K

RPA1 DBD-C/RPA2 DBD-D interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 618L, 622I
CDD
cd04476 (RPA1_DBD_C)

578N

RPA1 DBD-C/RPA2 DBD-D interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 618L, 622I
CDD
cd04476 (RPA1_DBD_C)

579R

RPA1 DBD-C/RPA2 DBD-D interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 618L, 622I
CDD
cd04476 (RPA1_DBD_C)

580C

RPA1 DBD-C/RPA2 DBD-D interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 618L, 622I
CDD
cd04476 (RPA1_DBD_C)

618L

RPA1 DBD-C/RPA2 DBD-D interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 618L, 622I
CDD
cd04476 (RPA1_DBD_C)

622I

RPA1 DBD-C/RPA2 DBD-D interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 618L, 622I
CDD
cd04476 (RPA1_DBD_C)
trimerization core...

481C

trimerization core interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 616L, 618L, 622I, 623E
CDD
cd04476 (RPA1_DBD_C)

534D

trimerization core interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 616L, 618L, 622I, 623E
CDD
cd04476 (RPA1_DBD_C)

535E

trimerization core interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 616L, 618L, 622I, 623E
CDD
cd04476 (RPA1_DBD_C)

575S

trimerization core interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 616L, 618L, 622I, 623E
CDD
cd04476 (RPA1_DBD_C)

576K

trimerization core interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 616L, 618L, 622I, 623E
CDD
cd04476 (RPA1_DBD_C)

578N

trimerization core interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 616L, 618L, 622I, 623E
CDD
cd04476 (RPA1_DBD_C)

579R

trimerization core interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 616L, 618L, 622I, 623E
CDD
cd04476 (RPA1_DBD_C)

580C

trimerization core interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 616L, 618L, 622I, 623E
CDD
cd04476 (RPA1_DBD_C)

616L

trimerization core interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 616L, 618L, 622I, 623E
CDD
cd04476 (RPA1_DBD_C)

618L

trimerization core interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 616L, 618L, 622I, 623E
CDD
cd04476 (RPA1_DBD_C)

622I

trimerization core interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 616L, 618L, 622I, 623E
CDD
cd04476 (RPA1_DBD_C)

623E

trimerization core interface
481C, 534D, 535E, 575S, 576K, 578N, 579R, 580C, 616L, 618L, 622I, 623E
CDD
cd04476 (RPA1_DBD_C)
Zinc binding site ...

493C

Zinc binding site
493C, 498C, 512C, 515C
CDD
cd04476 (RPA1_DBD_C)

498C

Zinc binding site
493C, 498C, 512C, 515C
CDD
cd04476 (RPA1_DBD_C)

512C

Zinc binding site
493C, 498C, 512C, 515C
CDD
cd04476 (RPA1_DBD_C)

515C

Zinc binding site
493C, 498C, 512C, 515C
CDD
cd04476 (RPA1_DBD_C)
RPA1 DBD-C/RPA3 in...

616L

RPA1 DBD-C/RPA3 interface
616L, 623E
CDD
cd04476 (RPA1_DBD_C)

623E

RPA1 DBD-C/RPA3 interface
616L, 623E
CDD
cd04476 (RPA1_DBD_C)

Protein sequence

>MCA_01192_1
MNTFSNPNNSLSTGFFQSLVDRVNDPSQHQNLPPVNLMILKCKGDASPSGPSRIRCLFSDGRHSLLGFFAPADRIEASLN
GKVVHLKAYAIKILQNKVCVIVRTFDILDEVVAPESIGSANLDSLVFPQNLEMTSLAQTEYQRQIQQHMNQQQQQQQQQQ
QKPAKEEKKINTNEYRNPNSRSESKSQASNYTAIADISPYSTKTVLHCRVSDRAPIRTFTNAKGEGKLFSATLVDESGEI
KLTGFNEQVDQFYEMLKPGEVFVMSQFRVTPAKKQFSNINHDYELMLTRNTVIEKSQSADDDKEIPTIHADFVALDKLGE
VAPDTVIDVLGVINQIDEVKELISKAGKPYTKRDIVLVDSSKSAVRATLWGEVAKTFEEKYQVGTVIALKGAKVSDFGGR
SLSLLFSGQVVDNITTPEAFKLSGWYAQSGNKEQFKFQNSGAAGSSTIDDTESYMLLGDVIENNIGKSEKADFFFARAIV
CSISPRYLYYPACTQPDCSKKVIFETDNTWRCEKCNMSIEKPNYRYTLSVAISDETNQIWVTVFDEVGQKILGVDANQLH
EYEEINSQKHNDVVSKFNRCPFVFQIRAKTEVFNDVPRVKYQVTRGSPVDSHKESLKLLKEIESY

GO term prediction

Biological Process

GO:0006260 DNA replication
GO:0006281 DNA repair
GO:0006310 DNA recombination

Molecular Function

GO:0003677 DNA binding

Cellular Component

GO:0005634 nucleus

Filter view on

Entry type

Status

Per-residue features