Protein

MCA_00881_1

Length
784 amino acids

Gene name: BRF1

Description: Transcription factor IIIB 70 kDa subunit

Browser: contigA:2776683-2779038+

RNA-seq: read pairs 1046, FPKM 16.5, percentile rank 36.2% (100% = highest expression)

Protein function

Annotation:BRF1Transcription factor IIIB 70 kDa subunit
KEGG:K15196BRF1 transcription factor IIIB 90 kDa subunit
EGGNOG:0PFE5BRF1transcription factor TFIIIB complex subunit Brf1
SGD closest match:S000003478BRF1Transcription factor IIIB 70 kDa subunit
CGD closest match:CAL0000177227TDS4Transcription factor IIIB 70 kDa subunit

Protein alignments

%idAln lengthE-value
MIA_05961_156.37%4246e-158MIA_05961_1
A0A0J9XJK6_GEOCN49.51%3058e-102Similar to Saccharomyces cerevisiae YGR246C BRF1 TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB OS=Geotrichum candidum GN=BN980_GECA24s00131g PE=4 SV=1
UniRef50_A0A0J9XJK649.51%3052e-98Similar to Saccharomyces cerevisiae YGR246C BRF1 TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XJK6_GEOCN
A0A167D3M9_9ASCO48.22%3093e-87Transcription factor TFIIIB subunit BRF1 OS=Sugiyamaella lignohabitans GN=BRF1 PE=4 SV=1
A0A060T3V2_BLAAD43.73%3115e-84ARAD1A19162p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A19162g PE=4 SV=1
A0A1E4TLF8_9ASCO44.83%2902e-82Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_30706 PE=4 SV=1
TF3B_YEAST40.06%3173e-77Transcription factor IIIB 70 kDa subunit OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BRF1 PE=1 SV=1
Q6C4S8_YARLI43.61%3056e-77YALI0E24035p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E24035g PE=4 SV=1
TF3B_CANAL36.31%3698e-74Transcription factor IIIB 70 kDa subunit OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=TDS4 PE=3 SV=1
A0A1E3PMX6_9ASCO43.38%1363e-28BRF1-domain-containing protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_45981 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0244

Protein family membership

Domains and repeats

  1. Domain
1 100 200 300 400 500 600 700 784

Detailed signature matches

  1. Family IPR000812
    Transcription factor TFIIB
    1. PR00685 (TIFACTORIIB)
  2. Domain IPR013763
    Cyclin-like
    1. G3DSA:1.10.47...
    2. SSF47954 (Cyclin-like)
    3. SM00385 (cyclin_7)
    4. cd00043 (CYCLIN)
  3. Domain IPR013150
    Transcription factor TFIIB, cyclin-like domain
    1. PF00382 (TFIIB)
  4. Domain IPR011665
    Brf1, TBP-binding domain
    1. PF07741 (BRF1)
  5. SITE IPR023486
    Transcription factor TFIIB, conserved site
    1. PS00782 (TFIIB)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:1.20.5.650
  3. mobidb-lite (disord...)

Residue annotation

  1. binding site 1 cd0...
  2. binding site 2 cd0...
  3. binding site 1 cd0...
  4. binding site 2 cd0...

Protein sequence

>MCA_00881_1
MDNSRKCGKCGGSLFNGTCTACGFVWEESVMVSDLTFGESSSGAAVVNGSFVSADRSGLDRIGNGLYGHGSANTKEITIE
KAKQRIQGVAYALKIPEPVINAAIQNFKYALRSNFIRGRKSKYVTCACLYLGCRQKQSSHLLMEFATFLKINVYTLGHTY
TQLLKAIYPIIGEKELKRTMFVDPSIYIQKFINRLNLTSDEGKKVYGDSHRIVRRMEKDWIIFGRRPAGVAAASVLIACR
MNNIKRSKAKIMQIAMVAESTIDKRLKEFSSTAIGSLSIKEFRASNVGADADPPSFARNRKLERELGKRSIDAANRGLEE
RADIVITETESGNLVDSEGRNALAIEGLDGENDLTDENILQMAEEARQLYEFEDDPENDESKIDLMRRIQEDHILAAGIG
KATLAAIENAKAELERAKARQGRKEEEPDANLEDPTAEENDEPVEQNDEEDEPVEKNDEDDEELFSAKEDEEDVEKENEE
EENEEEPAEVENEEPSEAENDEESSEIEAENENKEQEKEEEQEETSEVATKKNEENEAEDTSNDKSENEDDEDQMEVDEE
EKDDEDEENDGEDDDEDDIIKMDDEDAANSENGSDNEEDAKPKQATPVPPKSKEELRKSREERIAAILKRHNHPVSTLTQ
KITLSKANSKWENETDSKISDDPENLEDVDDAEIDGVICTPMEVQARERLWISLNKDYEIEQAHKKNKIEADKRDGIYKE
PRKRVRHTRTGWGKSNNNENGGDQSATSVAVQMMMKKSKVLRVAKKSKKINYNVYEDILKAAQD

GO term prediction

Biological Process

GO:0006355 regulation of transcription, DNA-templated
GO:0070897 DNA-templated transcriptional preinitiation complex assembly

Molecular Function

GO:0017025 TBP-class protein binding

Cellular Component

None predicted.