Protein

MCA_00874_1

Length
1,288 amino acids

Gene name: RAD2

Description: DNA repair protein RAD2

Browser: contigA:2753478-2757345-

RNA-seq: read pairs 789, FPKM 7.6, percentile rank 21.6% (100% = highest expression)

Protein function

Annotation:RAD2DNA repair protein RAD2
KEGG:K10846ERCC5 DNA excision repair protein ERCC-5
EGGNOG:0PGTDRAD2DNA excision repair protein
SGD closest match:S000003490RAD2DNA repair protein RAD2
CGD closest match:CAL0000187217RAD2SsDNA endodeoxyribonuclease

Protein alignments

%idAln lengthE-value
MIA_06080_157.33%3893e-120MIA_06080_1
A0A0J9XJW3_GEOCN53.03%3798e-106Similar to Saccharomyces cerevisiae YGR258C RAD2 Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA OS=Geotrichum candidum GN=BN980_GECA20s00714g PE=4 SV=1
UniRef50_A0A0J9XJW353.03%3792e-102Similar to Saccharomyces cerevisiae YGR258C RAD2 Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XJW3_GEOCN
A0A060T6D4_BLAAD47.93%3631e-86ARAD1B15004p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B15004g PE=4 SV=1
A0A167DVL9_9ASCO51.28%2731e-83SsDNA endodeoxyribonuclease RAD2 OS=Sugiyamaella lignohabitans GN=RAD2 PE=4 SV=1
Q6C8E7_YARLI43.15%3368e-70YALI0D20240p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_D20240g PE=4 SV=1
A0A1D8PLU8_CANAL41.38%3773e-70SsDNA endodeoxyribonuclease OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=RAD2 PE=4 SV=1
RAD2_YEAST50.19%2676e-69DNA repair protein RAD2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RAD2 PE=1 SV=2
A0A1E4TFR7_9ASCO51.52%1652e-46Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_19392 PE=4 SV=1
A0A1E3PHQ3_9ASCO55.63%1516e-44PIN domain-like protein (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_12063 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.4022

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
  3. Domain
1 200 400 600 800 1000 1288

Detailed signature matches

  1. Family IPR006084
    XPG/Rad2 endonuclease
    1. PR00853 (XPGRADSUPER)
  2. Family IPR001044
    XPG/Rad2 endonuclease, eukaryotes
    1. PR00066 (XRODRMPGMNTG)
  3. Domain IPR006085
    XPG N-terminal
    1. SM00485 (xpgn3)
    2. PF00752 (XPG_N)
  4. Domain IPR029060
    PIN domain-like
    1. G3DSA:3.40.50...
    2. SSF88723 (PIN domai...)
  5. Domain IPR006086
    XPG-I domain
    1. SM00484 (xpgineu)
    2. PF00867 (XPG_I)
  6. Domain IPR020045
    5'-3' exonuclease, C-terminal domain
    1. SSF47807 (5' to 3' ...)
  7. SITE IPR019974
    XPG conserved site
    1. PS00842 (XPG_2)
  8. SITE IPR008918
    Helix-hairpin-helix motif, class 2
    1. SM00279 (HhH_4)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:1.10.15...
  3. cd09868 (PIN_XPG)
  4. cd09904 (H3TH_XPG)
  5. mobidb-lite (disord...)

Residue annotation

  1. metal binding site...
  2. active site cd09868
  3. putative 5' ssDNA ...
  4. putative DNA bindi...
  5. putative metal bin...

Protein sequence

>MCA_00874_1
MGVRGLWEVVAPVAKPVNLDVMAGQRLAVDASIWIYHFLKAMRDSEGKVIRHAHVVGFLRRICKLIYFGIQPVFVFDGGA
PALKRSTIANRKERSRNRLETAQATAAKILTLQLHKHAMNMKKNAKKDKGKTVSSKDDKLLNDDDTEVAYLDEVSLDKSK
RTNVNKESKVEEMKRKTLSDPYFLPEVTQDDINRQMMELNDPRMLTQDELDTYADEFQSQIAALKTDLRNNNEDSVSGLY
DEKVMDFESAEFKALPITTQYQLLSAARLRSRLRMGYTIDQLENKFADKYEFSKFQIERVRQRNYLTQKLMNFSEEDAKG
IDSKVSGGGNTRTLTRRVAGQRDREYVLQRNDQGWTLALEDERISSNVIAIDVDDTEIRDSELQFQNKEKLRVESNKVSE
DDDDYDSDEIEWEDVVVKSPKEEEDYIDHQLEEVQDSKEEDLSKEHEVNTKTDNPFIIEESESDNEQDDSNFSNNDNQAE
STVSVQSGDININSLKPLSFKNSLFSKSTQKPSLLSKYSQSAPPKKQPENTIKKHTDKPVETLVKEPVKEPSQESTEETA
KKPSFDKDLPVWFQNSSVKTSKSDHEANNKNISNPQEREVLDMGESSFFRRPLKPLDPLIYSSTSSSEEDEEGSVELDDE
FIPYEELQRRRQRAVYQVENDYDDDEDEIQIVKETTKDDGDDDIIIVSETSTSKPTAEAEAPIPASFKYAETQKLDIDMT
KNLKKSANQELANQDTPVVSPSTSTLKSLYDGKTPTSDTPTKSAQKNNAAQHKESPKNNETPTKSKESAASQYTKEHLEE
LAKQEEEYQKQEDEDMLEQYQAEIEETKEYLDQNDTSSLPTTPTGTNKKKATFYHKPMSHYSSSSTTSFSNSSRAYPSES
SPAQRLQDMPDLYSPTNSSLDADIAQLRRQKNKEMRDADQVTPEMIAECQELLKRFGIPFITAPMEAEAQCAELQTLKLV
DGIITDDADVFLFGDNVRVFKNMFASLAGVGNGPSSGKKSQVIKYVECYDSADIRNELGLDRKKLIKLAYLLGSDYTEGV
TGIGPVSAMEILANFDTNEKENGLIKFKNWWKQVQLKRSTTTSGNNEAAAETEFERKFRKLNTTKIFLPTDFPNKEVEKA
YLEPEVDKDKTPFEWGTPDLDSLRKLCKSLLGWNEYKTDELLVPVIKMMNQRKTQSNARQTELKDFFMFPERDIARKSTD
GTARIAQNGKVTRSKRLNKAINVMSSRMKNNSASRQNSKDKDDSNKTRQEATETNQQQIQEEEDLYEQEGGFMETQRPQI
KRRKLNKK

GO term prediction

Biological Process

GO:0006281 DNA repair
GO:0006289 nucleotide-excision repair

Molecular Function

GO:0003677 DNA binding
GO:0003697 single-stranded DNA binding
GO:0003824 catalytic activity
GO:0004518 nuclease activity
GO:0004519 endonuclease activity
GO:0016788 hydrolase activity, acting on ester bonds

Cellular Component

GO:0005634 nucleus