Protein

MCA_00621_1

Length
536 amino acids

Gene name: HTS1

Description: Histidine--tRNA ligase, mitochondrial

Browser: contigA:1904181-1905792-

RNA-seq: read pairs 2597, FPKM 59.8, percentile rank 69.2% (100% = highest expression)

Protein function

Annotation:HTS1Histidine--tRNA ligase, mitochondrial
KEGG:K01892HARS histidyl-tRNA synthetase [EC:6.1.1.21]
EGGNOG:0PHWWHTS1histidyl-tRNA synthetase
SGD closest match:S000006237HTS1Histidine--tRNA ligase, mitochondrial
CGD closest match:CAL0000190585HTS1Histidine--tRNA ligase

Protein alignments

%idAln lengthE-value
MIA_01319_182.83%5300.0MIA_01319_1
A0A0J9X4T1_GEOCN83.17%5110.0Similar to Saccharomyces cerevisiae YPR033C HTS1 Cytoplasmic and mitochondrial histidine tRNA synthetase OS=Geotrichum candidum GN=BN980_GECA02s00582g PE=3 SV=1
A0A161HFB6_9ASCO76.71%5110.0Histidine--tRNA ligase OS=Sugiyamaella lignohabitans GN=HTS1 PE=3 SV=1
A0A1E3PKW8_9ASCO71.91%5090.0Histidyl-tRNA synthetase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_46520 PE=3 SV=1
A0A060T1A0_BLAAD72.12%5200.0ARAD1C18480p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C18480g PE=3 SV=1
Q6C624_YARLI69.90%5150.0YALI0E13123p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E13123g PE=3 SV=1
UniRef50_A0A1X7QXZ963.39%5080.0Similar to Saccharomyces cerevisiae YPR033C HTS1 Cytoplasmic and mitochondrial histidine tRNA synthetase n=2 Tax=Fungi TaxID=4751 RepID=A0A1X7QXZ9_9SACH
SYH_YEAST61.55%5150.0Histidine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HTS1 PE=1 SV=2
Q5AJX0_CANAL63.64%5170.0Histidine--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=HTS1 PE=3 SV=1
A0A1E4TM04_9ASCO65.06%4980.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_1385 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0217

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 50 100 150 200 250 300 350 400 450 500 536

Detailed signature matches

  1. Family IPR004516
    Histidine-tRNA ligase/ATP phosphoribosyltransferase regulatory subunit
    1. PIRSF001549 (His-tR...)
  2. Family IPR015807
    Histidine-tRNA ligase
    1. MF_00127 (His_tRNA_...)
  3. Domain IPR006195
    Aminoacyl-tRNA synthetase, class II
    1. PS50862 (AA_TRNA_LI...)
  4. Domain IPR004154
    Anticodon-binding
    1. SSF52954 (Class II ...)
    2. G3DSA:3.40.50...
    3. PF03129 (HGTP_antic...)
  5. Domain IPR033656
    Histidyl-anticodon-binding
    1. cd00859 (HisRS_anti...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.30.93...
  2. PF13393 (tRNA-synt_His)
  3. SSF55681 (Class II ...)
  4. cd00773 (HisRS-like...)
  5. mobidb-lite (disord...)

Residue annotation

  1. dimer interface cd...
  2. motif 1 cd00773
  3. active site cd00773
  4. motif 2 cd00773
  5. motif 3 cd00773
  6. anticodon binding ...

Protein sequence

>MCA_00621_1
MSESAAPAAAPAPEVKKTNKKEAKAAVQQFTLKTPKGTKDWSDKDMILRSAIFDTLTKVFKRHGGVTIDTPVFELREILS
GKYGEDSKLIYNLEDQGGELTSLRYDLTVPFARFVAMNGIQQIKRYHIAKVYRRDQPAMTKGRMREFYQCDFDIAGEYES
MVPDAEILSIAVEGLTALNIKDYTIKINHRKILDGIFELCGVKEEDIRKVSSAVDKLDKLPWEDVKKEMVVDKAQPEEVA
DKIGEYVRIKGSYKEVLDKLSQDEALMANASAAAGVQDMAKAYDYLVAFKVVDHLSFDLSLARGLDYYTGIIYEVVTAAS
APPPKPEPSAATEEPSKKSKKNKKSKEAQDDDDNSENVGVGSIMAGGRYDNLVGMFAGTSKKKNASIPCVGLSFGIERLF
SIIKAREGLMDKVRPTSTQVFVMAFGGGPSWDGFLKERMEVSRKLWDGGIEAEFIYKTKPKTQKQFEAAEKTGCPLAVIL
GQEEYSKGVVKVKELGLGADADQGVDVPVDEMVSYIKKKLNEKNNGIDEAIKLLSL

GO term prediction

Biological Process

GO:0006427 histidyl-tRNA aminoacylation

Molecular Function

GO:0004821 histidine-tRNA ligase activity
GO:0005524 ATP binding

Cellular Component

GO:0005737 cytoplasm