Protein

MCA_00445_1

Length
760 amino acids

Gene name: PUS7

Description: Pseudouridine synthase

Browser: contigA:1418246-1420732-

RNA-seq: read pairs 953, FPKM 15.5, percentile rank 34.8% (100% = highest expression)

Protein function

Annotation:PUS7Pseudouridine synthase
KEGG:K06176truD tRNA pseudouridine13 synthase [EC:5.4.99.27]
EGGNOG:0PFCWPGUG_03578pseudouridine synthase
SGD closest match:S000005769PUS7Multisubstrate pseudouridine synthase 7
CGD closest match:CAL0000177714PUS7Pseudouridine synthase

Protein alignments

%idAln lengthE-value
MIA_00665_167.80%6770.0MIA_00665_1
A0A0J9X8V1_GEOCN56.97%7250.0Similar to Saccharomyces cerevisiae YOR243C PUS7 Pseudouridine synthase, catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA,position 13 in cytoplasmic tRNA OS=Geotrichum candidum GN=BN980_GECA05s05059g PE=4 SV=1
A0A167D5T6_9ASCO51.91%7050.0Pseudouridine synthase PUS7 OS=Sugiyamaella lignohabitans GN=PUS7 PE=4 SV=1
UniRef50_A0A167D5T651.91%7050.0Pseudouridine synthase PUS7 n=3 Tax=Saccharomycetales TaxID=4892 RepID=A0A167D5T6_9ASCO
A0A060T788_BLAAD53.55%6760.0ARAD1B21582p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B21582g PE=4 SV=1
A0A1E3PPM4_9ASCO47.74%6850.0tRNA pseudouridine synthase D OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_81800 PE=4 SV=1
Q6CAL7_YARLI46.77%6650.0YALI0D01628p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_D01628g PE=4 SV=1
PUS7_YEAST46.17%6780.0Multisubstrate pseudouridine synthase 7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PUS7 PE=1 SV=1
A0A1D8PIN8_CANAL41.32%7268e-159Pseudouridine synthase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PUS7 PE=4 SV=1
A0A1E4TK59_9ASCO39.13%6674e-157Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_82618 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.4926

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 600 700 760

Detailed signature matches

  1. Family IPR001656
    Pseudouridine synthase, TruD
    1. PF01142 (TruD)
    2. TIGR00094
  2. Domain IPR020103
    Pseudouridine synthase, catalytic domain
    1. SSF55120 (Pseudouri...)
  3. Domain IPR011760
    Pseudouridine synthase, TruD, insertion domain
    1. PS50984 (TRUD)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.30.23...
  2. PIRSF037016 (Pseudo...)
  3. cd02576 (PseudoU_sy...)
  4. mobidb-lite (disord...)

Residue annotation

  1. probable active si...

Protein sequence

>MCA_00445_1
MKLNGYIAKLSAYINPIISKKSSSLYFLSNSKIMTSTTRPLDEDSSTQNNDPKRIKLETENESTSTEPSTSTSKVDDEAQ
EKKPVRENYSAHNQGISEQDVGITQYLHPELKGFKGILKQRYTDFLVNEIDKEGNVVYLTSYGFGDSTDPKTSEATEPAN
AEEEKKQTIDTSSSKTEEAAEEPLKEFKKKEFKLSDENREKLVALLGEATVDGMVALLTNGSKIVTETPIDDKDERTKIH
QTVREAFDSKLETRTTPESTFIITLANSNTRVNKNQPRRGKKNRRGKDSKNDAAATLGEKKDYLHFTLYKENKETMQVAN
IMSKYLRVPPKTVTYAGTKDRRGVTTQRACIYKMPAERVNGLNKALRGIKFGSFKYEDAQLKLGDLQGNEFYITIKNVDE
KNPENIVKSMECLRDDGFINYYGMQRFGTFSVSTHTIGSHILKSDWETAVNLILSPQELVIPESVEARKVYHETKDASKA
LELMPRRCVAESCILQVLAETPRSYFNAIMKIPRNLRIMYGHAYQSYIWNVVASERIKRYGKKIMEGDLVLIDDAEKKAN
LELLNSATAGEEGEEEIEEDVKQDIFIRARPVTKEEIETGAKTIFDVVLPTPGFDIRYPDNDLKQVYIDEMKKDGLDPDN
MRRNIREFSLAGSYRHLLSKPSAVQWWVKRYKNDTDSLVKTDVDLINEGATDFTNRIVEEFKDVVTPETDKEGDEEFNKI
AVLLKIQLGTSQYATMALRELMKLDTKRRGDGLDVRTQER

GO term prediction

Biological Process

GO:0001522 pseudouridine synthesis
GO:0009451 RNA modification

Molecular Function

GO:0003723 RNA binding
GO:0009982 pseudouridine synthase activity

Cellular Component

None predicted.