MCA_00438_1

Protein

Length

507 amino acids

Gene name: MCY1

Description: Putative cysteine synthetase

Browser: contigA:1381923-1383447+

RNA-seq: read pairs 311, FPKM 7.6, percentile rank 21.6% (100% = highest expression)

Protein function

Annotation:		MCY1	Putative cysteine synthetase
KEGG:	K01738	cysK	cysteine synthase A [EC:2.5.1.47]
EGGNOG:	0PIEF	FG00903.1	cysteine synthase
SGD closest match:	S000003244	YGR012W	Putative cysteine synthase
CGD closest match:	CAL0000195320	orf19.5574	Putative cysteine synthase

Protein alignments

	%id	Aln length	E-value
MIA_00531_1	65.02%	406	1e-179	MIA_00531_1
A0A0J9X3Z3_GEOCN	64.44%	405	1e-174	Similar to Saccharomyces cerevisiae YGR012W Putative cysteine synthase OS=Geotrichum candidum GN=BN980_GECA02s02870g PE=4 SV=1
A0A1E3PL92_9ASCO	59.90%	394	2e-162	PALP-domain-containing protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_82960 PE=4 SV=1
A0A161HFX7_9ASCO	58.64%	411	1e-162	Putative cysteine synthase OS=Sugiyamaella lignohabitans GN=AWJ20_2137 PE=4 SV=1
A0A060T7X3_BLAAD	60.63%	381	2e-158	ARAD1C31504p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C31504g PE=4 SV=1
Q6C6L3_YARLI	57.29%	384	2e-139	YALI0E08536p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E08536g PE=4 SV=1
A0A1E4TE82_9ASCO	58.24%	376	2e-138	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_1803 PE=4 SV=1
UniRef50_A3LRL7	51.37%	401	2e-134	Cysteine synthase (O-acetylserine sulfhydrylase) (O-acetylserine (Thiol)-lyase) (CSase) n=27 Tax=Fungi TaxID=4751 RepID=A3LRL7_PICST
A0A1D8PQ13_CANAL	52.51%	398	3e-136	Putative cysteine synthase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.5574 PE=4 SV=1
CYSKL_YEAST	51.91%	393	2e-128	Putative cysteine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YGR012W PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0229

Protein family membership

None predicted.

Domains and repeats

Domain
115 - 494
Tryptophan synthase beta subunit-like PLP-dependent enzyme (IPR001926)

1 50 100 150 200 250 300 350 400 450 507

Detailed signature matches

IPR001926

Tryptophan synthase beta subunit-like PLP-dependent enzyme
1. SSF53686 (Tryptopha...)
  
  290 - 494
  
  SUPERFAMILY
  SSF53686 (Tryptophan synthase beta subunit-like PLP-dependent enzymes)
  
  115 - 253
  
  SUPERFAMILY
  SSF53686 (Tryptophan synthase beta subunit-like PLP-dependent enzymes)
2. PF00291 (PALP)
  
  120 - 482
  
  Pfam
  PF00291 (PALP)
IPR001216

Cysteine synthase/cystathionine beta-synthase, pyridoxal-phosphate attachment site
1. PS00901 (CYS_SYNTHASE)
  
  144 - 162
  
  PROSITE patterns
  PS00901 (CYS_SYNTHASE)

no IPR

Unintegrated signatures

CYTOPLASMIC_D... (C...)

1 - 11

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)
Coil

242 - 262

COILS
Coil (Coil)

504 - 507

COILS
Coil (Coil)
G3DSA:3.40.50...

296 - 496

GENE3D
G3DSA:3.40.50.1100 (G3DSA:3.40.50.1100)

149 - 295

GENE3D
G3DSA:3.40.50.1100 (G3DSA:3.40.50.1100)
NON_CYTOPLASM... (N...)

36 - 507

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
TMhelix

15 - 37

TMHMM
TMhelix (TMhelix)
TRANSMEMBRANE (Tran...)

12 - 35

PHOBIUS
TRANSMEMBRANE (Transmembrane region)
cd01561 (CBS_like)

123 - 489

CDD
cd01561 (CBS_like)

Residue annotation

dimer interface cd...

127M

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

128I

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

129E

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

142L

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

148N

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

150P

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

195M

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

198R

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

214I

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

215E

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

218M

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

219L

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

222N

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

223L

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

316A

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

317Q

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

445V

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

446S

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

447N

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

449G

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

450V

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

451F

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

475T

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

485S

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)

488V

dimer interface
127M, 128I, 129E, 142L, 148N, 150P, 195M, 198R, 214I, 215E, 218M, 219L, 222N, 223L, 316A, 317Q, 445V, 446S, 447N, 449G, 450V, 451F, 475T, 485S, 488V
CDD
cd01561 (CBS_like)
catalytic residue ...

155K

catalytic residue
155K
CDD
cd01561 (CBS_like)
pyridoxal 5'-phosp...

155K

pyridoxal 5'-phosphate binding site
155K, 188S, 329A, 330G, 331T, 332G, 333G, 334T, 409G, 455S, 481C, 482D
CDD
cd01561 (CBS_like)

188S

pyridoxal 5'-phosphate binding site
155K, 188S, 329A, 330G, 331T, 332G, 333G, 334T, 409G, 455S, 481C, 482D
CDD
cd01561 (CBS_like)

329A

pyridoxal 5'-phosphate binding site
155K, 188S, 329A, 330G, 331T, 332G, 333G, 334T, 409G, 455S, 481C, 482D
CDD
cd01561 (CBS_like)

330G

pyridoxal 5'-phosphate binding site
155K, 188S, 329A, 330G, 331T, 332G, 333G, 334T, 409G, 455S, 481C, 482D
CDD
cd01561 (CBS_like)

331T

pyridoxal 5'-phosphate binding site
155K, 188S, 329A, 330G, 331T, 332G, 333G, 334T, 409G, 455S, 481C, 482D
CDD
cd01561 (CBS_like)

332G

pyridoxal 5'-phosphate binding site
155K, 188S, 329A, 330G, 331T, 332G, 333G, 334T, 409G, 455S, 481C, 482D
CDD
cd01561 (CBS_like)

333G

pyridoxal 5'-phosphate binding site
155K, 188S, 329A, 330G, 331T, 332G, 333G, 334T, 409G, 455S, 481C, 482D
CDD
cd01561 (CBS_like)

334T

pyridoxal 5'-phosphate binding site
155K, 188S, 329A, 330G, 331T, 332G, 333G, 334T, 409G, 455S, 481C, 482D
CDD
cd01561 (CBS_like)

409G

pyridoxal 5'-phosphate binding site
155K, 188S, 329A, 330G, 331T, 332G, 333G, 334T, 409G, 455S, 481C, 482D
CDD
cd01561 (CBS_like)

455S

pyridoxal 5'-phosphate binding site
155K, 188S, 329A, 330G, 331T, 332G, 333G, 334T, 409G, 455S, 481C, 482D
CDD
cd01561 (CBS_like)

481C

pyridoxal 5'-phosphate binding site
155K, 188S, 329A, 330G, 331T, 332G, 333G, 334T, 409G, 455S, 481C, 482D
CDD
cd01561 (CBS_like)

482D

pyridoxal 5'-phosphate binding site
155K, 188S, 329A, 330G, 331T, 332G, 333G, 334T, 409G, 455S, 481C, 482D
CDD
cd01561 (CBS_like)

Protein sequence

>MCA_00438_1
MPSSSSSPSSYFPIVMASTTTVAALMTAVYTYYYLLANEPANDEKKAKHHPEGITIFATLDSQADPISQCLDSGNPDDFD
RYFSKYTYTNDTGDTTDKQVLTIPKYQLGNLQPLRSGIDTLIGNTPMIELHSLSKATGCRVLAKMEMNNPGGSAKDRVAL
AIIEKAEREGLITPHKYGDIIFEGTSGSTGISLAMLCRAKGYNCHIALPDDTSIEKIMLLENLGATVYKVRPASIIDGNQ
YVNFARNKAEEINKYCKKVEKEYYEKKKQQQEQNFGDKNIEEEEFIRPPRALFSDQFENEANWQTHFENTGPEIYAQTQG
NLDVFVTGAGTGGTISGVARYLKQGNRHLKKITQQQQSSSSTKKSQLRVILADPQGSGFYNRIKFGAMFSPTEKEGTRKR
HQVDTIVEGIGLNRLTKNFLAGCDYIDDAVRVTDREALLMAKWLVSNDGVFIGSSSAVNAVAAYRCAKALGPGHTIVTLL
CDSGSRHVSKFWKEAESLTPLSSLDEL

GO term prediction

Biological Process

GO:0006535 cysteine biosynthetic process from serine

Molecular Function

None predicted.

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

MCA_00438_1

Protein function

Protein alignments

Mitochondrial localization by mitoprotII

Protein family membership

Domains and repeats

Detailed signature matches

Residue annotation

Protein sequence

GO term prediction

Biological Process

Molecular Function

Cellular Component