MCA_00291_1

Protein

Length

1,472 amino acids

Gene name: SLN1B

Description: Osmosensing histidine protein kinase Sln1; in S. cerevisiae transmembrane histidine phosphotransfer kinase and osmosensor that regulates MAP kinase cascade.

Browser: contigA:880907-885391-

RNA-seq: read pairs 649, FPKM 5.4, percentile rank 18.4% (100% = highest expression)

Protein function

Annotation:		SLN1B	Osmosensing histidine protein kinase Sln1; in S. cerevisiae transmembrane histidine phosphotransfer kinase and osmosensor that regulates MAP kinase cascade.
EGGNOG:	0PFPP	SLN1	Histidine kinase
SGD closest match:	S000001409	SLN1	Osmosensing histidine protein kinase SLN1
CGD closest match:	CAL0000175114	SLN1	Histidine protein kinase SLN1

Protein alignments

	%id	Aln length	E-value
MIA_01337_1	58.90%	438	6e-136	MIA_01337_1
A0A0J9XIR5_GEOCN	44.66%	459	4e-86	Similar to Saccharomyces cerevisiae YIL147C SLN1 Histidine kinase osmosensor that regulates a MAP kinase cascade OS=Geotrichum candidum GN=BN980_GECA19s02012g PE=4 SV=1
UniRef50_A0A0J9XIR5	44.66%	459	9e-83	Similar to Saccharomyces cerevisiae YIL147C SLN1 Histidine kinase osmosensor that regulates a MAP kinase cascade n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XIR5_GEOCN
A0A060T557_BLAAD	39.10%	491	5e-79	ARAD1C09284p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C09284g PE=4 SV=1
A0A167CAF7_9ASCO	36.40%	456	3e-59	Sln1p OS=Sugiyamaella lignohabitans GN=SLN1 PE=4 SV=1
Q6C2D5_YARLI	51.26%	199	1e-43	YALI0F08789p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F08789g PE=4 SV=1
SLN1_CANAL	43.35%	203	7e-41	Histidine protein kinase SLN1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=SLN1 PE=1 SV=1
A0A1E3PF83_9ASCO	51.61%	186	2e-39	Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_43709 PE=4 SV=1
A0A1E4TA96_9ASCO	46.31%	203	2e-38	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_127796 PE=4 SV=1
SLN1_YEAST	50.00%	122	3e-36	Osmosensing histidine protein kinase SLN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SLN1 PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.2934
Predicted cleavage: 86

Protein family membership

None predicted.

Domains and repeats

Domain
681 - 760
Signal transduction histidine kinase, dimerisation/phosphoacceptor domain (IPR003661)
999 - 1,013
Signal transduction histidine kinase-related protein, C-terminal (IPR004358)
1,017 - 1,027
Signal transduction histidine kinase-related protein, C-terminal (IPR004358)
1,034 - 1,052
Signal transduction histidine kinase-related protein, C-terminal (IPR004358)
1,058 - 1,071
Signal transduction histidine kinase-related protein, C-terminal (IPR004358)
1,314 - 1,438
CheY-like superfamily (IPR011006)
Signal transduction response regulator, receiver domain (IPR001789)
Domain
702 - 1,074
Histidine kinase domain (IPR005467)
Domain
749 - 1,081
Histidine kinase-like ATPase, C-terminal domain (IPR003594)

1 200 400 600 800 1000 1200 1472

Detailed signature matches

IPR003661

Signal transduction histidine kinase, dimerisation/phosphoacceptor domain
1. cd00082 (HisKA)
  
  693 - 756
  
  CDD
  cd00082 (HisKA)
2. SSF47384 (Homodimer...)
  
  681 - 760
  
  SUPERFAMILY
  SSF47384 (Homodimeric domain of signal transducing histidine kinase)
3. SM00388 (HisKA_10)
  
  695 - 760
  
  SMART
  SM00388 (HisKA_10)
4. PF00512 (HisKA)
  
  695 - 758
  
  Pfam
  PF00512 (HisKA)
IPR005467

Histidine kinase domain
1. PS50109 (HIS_KIN)
  
  702 - 1,074
  
  PROSITE profiles
  PS50109 (HIS_KIN)
IPR003594

Histidine kinase-like ATPase, C-terminal domain
1. G3DSA:3.30.56...
  
  764 - 1,081
  
  GENE3D
  G3DSA:3.30.565.10 (G3DSA:3.30.565.10)
2. PF02518 (HATPase_c)
  
  999 - 1,073
  
  Pfam
  PF02518 (HATPase_c)
3. SSF55874 (ATPase do...)
  
  749 - 842
  
  SUPERFAMILY
  SSF55874 (ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase)
  
  993 - 1,076
  
  SUPERFAMILY
  SSF55874 (ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase)
4. SM00387 (HKATPase_4)
  
  810 - 1,074
  
  SMART
  SM00387 (HKATPase_4)
IPR004358

Signal transduction histidine kinase-related protein, C-terminal
1. PR00344 (BCTRLSENSOR)
  
  1,017 - 1,027
  
  PRINTS
  PR00344 (BCTRLSENSOR)
  
  1,034 - 1,052
  
  PRINTS
  PR00344 (BCTRLSENSOR)
  
  999 - 1,013
  
  PRINTS
  PR00344 (BCTRLSENSOR)
  
  1,058 - 1,071
  
  PRINTS
  PR00344 (BCTRLSENSOR)
IPR011006

CheY-like superfamily
1. SSF52172 (CheY-like)
  
  1,314 - 1,437
  
  SUPERFAMILY
  SSF52172 (CheY-like)
IPR001789

Signal transduction response regulator, receiver domain
1. PS50110 (RESPONSE_R...)
  
  1,317 - 1,438
  
  PROSITE profiles
  PS50110 (RESPONSE_REGULATORY)
2. PF00072 (Response_reg)
  
  1,318 - 1,433
  
  Pfam
  PF00072 (Response_reg)
3. cd00156 (REC)
  
  1,319 - 1,437
  
  CDD
  cd00156 (REC)
4. SM00448 (REC_2)
  
  1,316 - 1,434
  
  SMART
  SM00448 (REC_2)

no IPR

Unintegrated signatures

CYTOPLASMIC_D... (C...)

1 - 11

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)

363 - 368

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)
Coil

1,471 - 1,472

COILS
Coil (Coil)
G3DSA:1.10.28...

686 - 756

GENE3D
G3DSA:1.10.287.130 (G3DSA:1.10.287.130)
G3DSA:3.40.50...

1,303 - 1,438

GENE3D
G3DSA:3.40.50.2300 (G3DSA:3.40.50.2300)
NON_CYTOPLASM... (N...)

35 - 343

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)

389 - 1,472

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
TMhelix

105 - 127

TMHMM
TMhelix (TMhelix)

12 - 34

TMHMM
TMhelix (TMhelix)

369 - 388

TMHMM
TMhelix (TMhelix)
TRANSMEMBRANE (Tran...)

344 - 362

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

369 - 388

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

12 - 34

PHOBIUS
TRANSMEMBRANE (Transmembrane region)
cd00075 (HATPase_c)

998 - 1,070

CDD
cd00075 (HATPase_c)
mobidb-lite (disord...)

1,448 - 1,472

MobiDB Lite
mobidb-lite (disorder_prediction)

237 - 278

MobiDB Lite
mobidb-lite (disorder_prediction)

478 - 639

MobiDB Lite
mobidb-lite (disorder_prediction)

410 - 437

MobiDB Lite
mobidb-lite (disorder_prediction)

1,120 - 1,151

MobiDB Lite
mobidb-lite (disorder_prediction)

951 - 989

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

dimer interface cd...

699F

dimer interface
699F, 703I, 707L, 710P, 714I, 717M, 733N, 737I, 740S, 744L, 747L, 751L
CDD
cd00082 (HisKA)

703I

dimer interface
699F, 703I, 707L, 710P, 714I, 717M, 733N, 737I, 740S, 744L, 747L, 751L
CDD
cd00082 (HisKA)

707L

dimer interface
699F, 703I, 707L, 710P, 714I, 717M, 733N, 737I, 740S, 744L, 747L, 751L
CDD
cd00082 (HisKA)

710P

dimer interface
699F, 703I, 707L, 710P, 714I, 717M, 733N, 737I, 740S, 744L, 747L, 751L
CDD
cd00082 (HisKA)

714I

dimer interface
699F, 703I, 707L, 710P, 714I, 717M, 733N, 737I, 740S, 744L, 747L, 751L
CDD
cd00082 (HisKA)

717M

dimer interface
699F, 703I, 707L, 710P, 714I, 717M, 733N, 737I, 740S, 744L, 747L, 751L
CDD
cd00082 (HisKA)

733N

dimer interface
699F, 703I, 707L, 710P, 714I, 717M, 733N, 737I, 740S, 744L, 747L, 751L
CDD
cd00082 (HisKA)

737I

dimer interface
699F, 703I, 707L, 710P, 714I, 717M, 733N, 737I, 740S, 744L, 747L, 751L
CDD
cd00082 (HisKA)

740S

dimer interface
699F, 703I, 707L, 710P, 714I, 717M, 733N, 737I, 740S, 744L, 747L, 751L
CDD
cd00082 (HisKA)

744L

dimer interface
699F, 703I, 707L, 710P, 714I, 717M, 733N, 737I, 740S, 744L, 747L, 751L
CDD
cd00082 (HisKA)

747L

dimer interface
699F, 703I, 707L, 710P, 714I, 717M, 733N, 737I, 740S, 744L, 747L, 751L
CDD
cd00082 (HisKA)

751L

dimer interface
699F, 703I, 707L, 710P, 714I, 717M, 733N, 737I, 740S, 744L, 747L, 751L
CDD
cd00082 (HisKA)
phosphorylation si...

705H

phosphorylation site
705H
CDD
cd00082 (HisKA)
ATP binding site c...

1,003V

ATP binding site
1,003V, 1,005D, 1,007G, 1,009G, 1,010I, 1,037G, 1,038L, 1,039G, 1,040L, 1,056L, 1,058N, 1,063G, 1,064L, 1,066V
CDD
cd00075 (HATPase_c)

1,005D

ATP binding site
1,003V, 1,005D, 1,007G, 1,009G, 1,010I, 1,037G, 1,038L, 1,039G, 1,040L, 1,056L, 1,058N, 1,063G, 1,064L, 1,066V
CDD
cd00075 (HATPase_c)

1,007G

ATP binding site
1,003V, 1,005D, 1,007G, 1,009G, 1,010I, 1,037G, 1,038L, 1,039G, 1,040L, 1,056L, 1,058N, 1,063G, 1,064L, 1,066V
CDD
cd00075 (HATPase_c)

1,009G

ATP binding site
1,003V, 1,005D, 1,007G, 1,009G, 1,010I, 1,037G, 1,038L, 1,039G, 1,040L, 1,056L, 1,058N, 1,063G, 1,064L, 1,066V
CDD
cd00075 (HATPase_c)

1,010I

ATP binding site
1,003V, 1,005D, 1,007G, 1,009G, 1,010I, 1,037G, 1,038L, 1,039G, 1,040L, 1,056L, 1,058N, 1,063G, 1,064L, 1,066V
CDD
cd00075 (HATPase_c)

1,037G

ATP binding site
1,003V, 1,005D, 1,007G, 1,009G, 1,010I, 1,037G, 1,038L, 1,039G, 1,040L, 1,056L, 1,058N, 1,063G, 1,064L, 1,066V
CDD
cd00075 (HATPase_c)

1,038L

ATP binding site
1,003V, 1,005D, 1,007G, 1,009G, 1,010I, 1,037G, 1,038L, 1,039G, 1,040L, 1,056L, 1,058N, 1,063G, 1,064L, 1,066V
CDD
cd00075 (HATPase_c)

1,039G

ATP binding site
1,003V, 1,005D, 1,007G, 1,009G, 1,010I, 1,037G, 1,038L, 1,039G, 1,040L, 1,056L, 1,058N, 1,063G, 1,064L, 1,066V
CDD
cd00075 (HATPase_c)

1,040L

ATP binding site
1,003V, 1,005D, 1,007G, 1,009G, 1,010I, 1,037G, 1,038L, 1,039G, 1,040L, 1,056L, 1,058N, 1,063G, 1,064L, 1,066V
CDD
cd00075 (HATPase_c)

1,056L

ATP binding site
1,003V, 1,005D, 1,007G, 1,009G, 1,010I, 1,037G, 1,038L, 1,039G, 1,040L, 1,056L, 1,058N, 1,063G, 1,064L, 1,066V
CDD
cd00075 (HATPase_c)

1,058N

ATP binding site
1,003V, 1,005D, 1,007G, 1,009G, 1,010I, 1,037G, 1,038L, 1,039G, 1,040L, 1,056L, 1,058N, 1,063G, 1,064L, 1,066V
CDD
cd00075 (HATPase_c)

1,063G

ATP binding site
1,003V, 1,005D, 1,007G, 1,009G, 1,010I, 1,037G, 1,038L, 1,039G, 1,040L, 1,056L, 1,058N, 1,063G, 1,064L, 1,066V
CDD
cd00075 (HATPase_c)

1,064L

ATP binding site
1,003V, 1,005D, 1,007G, 1,009G, 1,010I, 1,037G, 1,038L, 1,039G, 1,040L, 1,056L, 1,058N, 1,063G, 1,064L, 1,066V
CDD
cd00075 (HATPase_c)

1,066V

ATP binding site
1,003V, 1,005D, 1,007G, 1,009G, 1,010I, 1,037G, 1,038L, 1,039G, 1,040L, 1,056L, 1,058N, 1,063G, 1,064L, 1,066V
CDD
cd00075 (HATPase_c)
G-X-G motif cd00075

1,007G

G-X-G motif
1,007G, 1,009G, 1,037G, 1,039G
CDD
cd00075 (HATPase_c)

1,009G

G-X-G motif
1,007G, 1,009G, 1,037G, 1,039G
CDD
cd00075 (HATPase_c)

1,037G

G-X-G motif
1,007G, 1,009G, 1,037G, 1,039G
CDD
cd00075 (HATPase_c)

1,039G

G-X-G motif
1,007G, 1,009G, 1,037G, 1,039G
CDD
cd00075 (HATPase_c)
active site cd00156

1,322E

active site
1,322E, 1,323D, 1,372D, 1,380G, 1,401T, 1,420F, 1,423K, 1,424P
CDD
cd00156 (REC)

1,323D

active site
1,322E, 1,323D, 1,372D, 1,380G, 1,401T, 1,420F, 1,423K, 1,424P
CDD
cd00156 (REC)

1,372D

active site
1,322E, 1,323D, 1,372D, 1,380G, 1,401T, 1,420F, 1,423K, 1,424P
CDD
cd00156 (REC)

1,380G

active site
1,322E, 1,323D, 1,372D, 1,380G, 1,401T, 1,420F, 1,423K, 1,424P
CDD
cd00156 (REC)

1,401T

active site
1,322E, 1,323D, 1,372D, 1,380G, 1,401T, 1,420F, 1,423K, 1,424P
CDD
cd00156 (REC)

1,420F

active site
1,322E, 1,323D, 1,372D, 1,380G, 1,401T, 1,420F, 1,423K, 1,424P
CDD
cd00156 (REC)

1,423K

active site
1,322E, 1,323D, 1,372D, 1,380G, 1,401T, 1,420F, 1,423K, 1,424P
CDD
cd00156 (REC)

1,424P

active site
1,322E, 1,323D, 1,372D, 1,380G, 1,401T, 1,420F, 1,423K, 1,424P
CDD
cd00156 (REC)
phosphorylation si...

1,372D

phosphorylation site
1,372D
CDD
cd00156 (REC)
intermolecular rec...

1,375M

intermolecular recognition site
1,375M, 1,376P, 1,378M, 1,379D, 1,380G
CDD
cd00156 (REC)

1,376P

intermolecular recognition site
1,375M, 1,376P, 1,378M, 1,379D, 1,380G
CDD
cd00156 (REC)

1,378M

intermolecular recognition site
1,375M, 1,376P, 1,378M, 1,379D, 1,380G
CDD
cd00156 (REC)

1,379D

intermolecular recognition site
1,375M, 1,376P, 1,378M, 1,379D, 1,380G
CDD
cd00156 (REC)

1,380G

intermolecular recognition site
1,375M, 1,376P, 1,378M, 1,379D, 1,380G
CDD
cd00156 (REC)
dimerization inter...

1,423K

dimerization interface
1,423K, 1,424P, 1,425I
CDD
cd00156 (REC)

1,424P

dimerization interface
1,423K, 1,424P, 1,425I
CDD
cd00156 (REC)

1,425I

dimerization interface
1,423K, 1,424P, 1,425I
CDD
cd00156 (REC)

Protein sequence

>MCA_00291_1
MRSLKIGIRPQVILFVLFTVVLSLIILAVTTSLYCSNFILNLRTERLQVVSSLKSAQVLQAFSTIYYQTYLISTRDQLTQ
AMTRYRAGNNTESNWKEVASYFNNTFAASTIVSAAAIYDTNGYLLFFQSNDSSPYDSYNKDLFPISSDSPLVINQMYSAE
GYLTSPKVNNSQYLMTITFPIMGNSSFFVDSAKLYGYISLITLTTSYRSIIEDTTGLENGGLMYFLGFIDKDLNLQTAEP
PQNTTEPSHHRTQTIHKEEYEKASEETTEPTQQTGTANNNTYELLLPVTGQKYIEKSEQGPLDANSIPDLYDAIQQYDTG
SILKTTVFNQTNVAVGYSKVGNGLVTWVVIISEPLSSVYAPVAKLRDACLASGFGVLGVLFVIVVFLINKGVQPIYRLKQ
AAEQTTEYFHGISPDEGKGSTLISPSNSSKHDHQQPAINEKSLLQIDKIESNKVKRSHLLRLFFKSVFFSDKRKLKKGSK
SKKGLKTQNSSYEGKSTPKNSESSATSISSSDDNLKSNALKDSNYSHISSTNSPHSSATAASTSQSIIKKSRSANSMRKT
SESTINKNNHPTSTVPEISLDDKQSKLEIKERLENRDSQVEQNDEENDEDTDEPTEDEKKRNEQCKTDHSHPMAPPHVNI
KTPKYFTDELVSLQHSFNRMADELDKQYLHLEDMVRERTKELESARVQAEYANEAKSQFIATITHELRTPLNGILGMTAV
SLNEAEIPKVKKNLQVISKSGEVLLHLINDLLTFSKNQVGNVTLDEREFMLSEIILSLQKTYSKRSNSKKNLSLQYHFEP
SNLKNMVVLGDKGRITHVLTNIMLNSLKFAPNDTNISVSFRCIEFMGKIVNPTVPLEPRNGQINFIDTTCDDYLGGMSFT
NASQTSSYFTDTGTSGAAVSAIGGDASVTPSATTSFTSDAVPPIFHSPESPRSFTSNANNFSLAGNEQSEITPLHSDTGS
NISQFSSAISGNSNIGPENTHSKAHNPLTTYNQPQHCMFEFQVTDQGPGISESILDHVFEPFVQEDQALSRRYGGAGLGL
SICKQLVELMGGNISLKNSPQNGLSVTFQIPLEITRSFAYTPDPGFVFAEIPGIALLTRKRESVALLPSTTLTKGTTQLT
HSSSNGLVNNNVNDTRKDNALVPLSGSANVPTMTLSDPDGPDTSHVLNESHITAAATSLHGFKLPKQEGAKEAPQGSYFD
HKPVASGPSNHTPNLTGVDYFRNGSSSHIDMPSPFMINTTESGSIPVTPGSTATISTIRTTTGAHNFPTMVGTPGPNPTN
SYNGAFSNNGTPSTLSMTPTVNHSNTTTSMSTPSSIRILVAEDNLINQDIIRRMLRLEGIKDIDMAVNGEQAVLKIEDAI
RLGVYYNIMFLDVQMPRMDGLQAARIIRNQLGYPYPIVALTAYADEDNARQCYDAGMNAFLEKPIMRERLKEVLIQYSPP
GTFTHAYPTSSSIGTTTTPTGQETISATSTNE

GO term prediction

Biological Process

GO:0000160 phosphorelay signal transduction system
GO:0007165 signal transduction
GO:0016310 phosphorylation

Molecular Function

GO:0000155 phosphorelay sensor kinase activity
GO:0016772 transferase activity, transferring phosphorus-containing groups

Cellular Component