Protein

MCA_00230_1

Length
604 amino acids

Gene name: PUS1

Description: tRNA pseudouridine synthase 1

Browser: contigA:710347-712226+

RNA-seq: read pairs 1776, FPKM 36.3, percentile rank 58.1% (100% = highest expression)

Protein function

Annotation:PUS1tRNA pseudouridine synthase 1
KEGG:K06173truA tRNA pseudouridine38-40 synthase [EC:5.4.99.12]
EGGNOG:0PGFRPUS1tRNA pseudouridine synthase
SGD closest match:S000006133PUS1tRNA pseudouridine synthase 1
CGD closest match:CAL0000183350PUS1tRNA pseudouridine synthase 1

Protein alignments

%idAln lengthE-value
MIA_01278_172.12%2693e-129MIA_01278_1
A0A0J9XET1_GEOCN59.70%2686e-95Similar to Saccharomyces cerevisiae YPL212C PUS1 tRNA:pseudouridine synthase OS=Geotrichum candidum GN=BN980_GECA13s02089g PE=4 SV=1
UniRef50_A0A0J9XET159.70%2681e-91Similar to Saccharomyces cerevisiae YPL212C PUS1 tRNA:pseudouridine synthase n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XET1_GEOCN
A0A167DBI4_9ASCO64.45%2111e-91tRNA pseudouridine synthase OS=Sugiyamaella lignohabitans GN=PUS1 PE=3 SV=1
A0A060TGU0_BLAAD63.38%2138e-90tRNA pseudouridine synthase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D33506g PE=3 SV=1
A0A1E3PPH6_9ASCO63.13%1983e-86Pseudouridine synthase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_45384 PE=4 SV=1
PUS1_YARLI58.77%2284e-78tRNA pseudouridine synthase 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=PUS1 PE=3 SV=1
PUS1_CANAL62.01%1793e-72tRNA pseudouridine synthase 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PUS1 PE=3 SV=2
PUS1_YEAST60.21%1914e-72tRNA pseudouridine synthase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PUS1 PE=1 SV=1
A0A1E4TK33_9ASCO52.71%2033e-67tRNA pseudouridine synthase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_30361 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0084

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 604

Detailed signature matches

  1. Family IPR001406
    Pseudouridine synthase I, TruA
    1. TIGR00071
  2. Domain IPR020103
    Pseudouridine synthase, catalytic domain
    1. SSF55120 (Pseudouri...)
  3. Domain IPR020097
    Pseudouridine synthase I, TruA, alpha/beta domain
    1. PF01416 (PseudoU_sy...)
  4. Domain IPR020095
    Pseudouridine synthase I, TruA, C-terminal
    1. G3DSA:3.30.70...
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:3.30.70...
  3. cd02568 (PseudoU_sy...)
  4. mobidb-lite (disord...)

Residue annotation

  1. probable active si...

Protein sequence

>MCA_00230_1
MAENNSYRKNKWNKPRTNDRRTKKRRIDEHEERKERRDNSREWGPREDNGEPREPRRPKRKVAVMIGYCGTGYHGMQINP
PHKTIEEDLYKAFVEAGAISKDNASDLRKSSFMRAARTDKGVHAAGNVVSLKLIIEDPDVVEKINSHLPDQIRVWGISRT
NKAFECRKMCSSRIYEYLIPSYSFLPPKPESALAKSLAEADKQYPGVKRNDPEGIKFWEDVKQELLASGITEEDINRVQK
VSEDAEHKQLEQTRQKILESREKAKKELEERLKAKEETPEQPKEGDEEQKTEKAKETSESKEMESEETETKVAEESKEAE
SQEAEDPKEESENKEEDATDNSNKSELKFSEIVKKIKTVEREARRNYRISKERLDLIRQVLKQYEGVHNFHNFTIQKTFR
DPSAQRNMKTLSVSEPKIIENTEWLSIKIHGQSFMMHQIRKMIAMATLIVRCGTPITRIQDAFGNRSFNIPKAPGLGLLL
EQPVYDAYNSRLDALGYEPINFEKCKDEVEAFKFKHIYDKIYGEEDREHVFYKFFSFIDSFKGDATFEYLAPGPEDVGSS
QISTATKVAVGAAATAGAAAAVSAFVMADDEEDDELPVDGDTEG

GO term prediction

Biological Process

GO:0001522 pseudouridine synthesis
GO:0009451 RNA modification

Molecular Function

GO:0003723 RNA binding
GO:0009982 pseudouridine synthase activity

Cellular Component

None predicted.