Protein

MCA_00098_1

Length
1,752 amino acids


Gene name: RPO21

Description: DNA-directed RNA polymerase II subunit

Browser: contigA:262464-268026-

RNA-seq: read pairs 9819, FPKM 69.2, percentile rank 72.4% (100% = highest expression)

Protein function

Annotation:RPO21DNA-directed RNA polymerase II subunit
KEGG:K03006RPB1 DNA-directed RNA polymerase II subunit RPB1 [EC:2.7.7.6]
EGGNOG:0PGUDRPB1DNA-directed RNA Polymerase
SGD closest match:S000002299RPO21DNA-directed RNA polymerase II subunit RPB1
CGD closest match:CAL0000175853RPO21DNA-directed RNA polymerase subunit

Protein alignments

%idAln lengthE-value
MIA_04156_184.98%15780.0MIA_04156_1
A0A0J9X9V1_GEOCN80.29%15880.0DNA-directed RNA polymerase subunit OS=Geotrichum candidum GN=BN980_GECA06s03783g PE=3 SV=1
A0A1E3PRZ2_9ASCO71.77%15730.0DNA-directed RNA polymerase subunit OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_44819 PE=3 SV=1
A0A060T820_BLAAD72.06%15710.0DNA-directed RNA polymerase subunit OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C32846g PE=3 SV=1
Q6CBQ3_YARLI71.29%15640.0DNA-directed RNA polymerase subunit OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_C16566g PE=3 SV=2
A0A167FRX0_9ASCO71.91%15060.0DNA-directed RNA polymerase subunit OS=Sugiyamaella lignohabitans GN=RPO21 PE=3 SV=1
A0A1E4TC04_9ASCO69.93%15530.0DNA-directed RNA polymerase subunit OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_65867 PE=3 SV=1
A0A1D8PUA6_CANAL67.58%16040.0DNA-directed RNA polymerase subunit OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=RPO21 PE=3 SV=1
RPB1_YEAST63.86%15690.0DNA-directed RNA polymerase II subunit RPB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPO21 PE=1 SV=2
UniRef50_P0405063.86%15690.0DNA-directed RNA polymerase II subunit RPB1 n=761 Tax=Eukaryota TaxID=2759 RepID=RPB1_YEAST

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0692

Protein family membership

None predicted.

Domains and repeats

  1. Domain
  2. Domain
  3. Repeat
1 200 400 600 800 1000 1200 1400 1600 1752

Detailed signature matches

    1. PF04997 (RNA_pol_Rp...)
    1. SM00663 (rpolaneu7)
    1. PF00623 (RNA_pol_Rp...)
    1. PF04983 (RNA_pol_Rp...)
    1. PF05000 (RNA_pol_Rp...)
    1. PF04998 (RNA_pol_Rp...)
    1. PF04992 (RNA_pol_Rp...)
    1. PF04990 (RNA_pol_Rp...)
    1. PF05001 (RNA_pol_Rp...)
    2. PS00115 (RNA_POL_II...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. SSF64484 (beta and ...)
  2. cd02584 (RNAP_II_Rp...)
  3. cd02733 (RNAP_II_RP...)
  4. mobidb-lite (disord...)

Residue annotation

  1. clamp cd02733
  2. RPB1-TFIIB interfa...
  3. Zn-binding cd02733
  4. RPB1 - RPB2 interf...
  5. active site region...
  6. catalytic site cd0...
  7. Rpb1 - Rpb6 intera...
  8. cleft cd02584
  9. Rpb1 - Rpb2 intera...
  10. DNA binding site c...
  11. Rpb1 - Rpb5 intera...
  12. clamp cd02584

Protein sequence

>MCA_00098_1
MSGGRFPYSSAPLETIKEAYSIWSFVSRRNSISVVKIEFPETMDETRQRPRIQGLNDPRLGTIDRNFRCQTCGESMAECP
GHFGHIELAKPVFHIGFINKIKKVCESVCLNCGKLLLDESNPLFAQAISIRDPKRRFNAVWALCKQKMICEADPPEEDYN
ALNSHKGERHSHGGCGNSQPTIRRDGLKLVGTWKREKGEDGEVIQDKRTLTPSEILNIFKHISSEDIYRLGLNEDYARPE
WMIITVLPVPPPPVRPSIAINESARGEDDLTYKLADIIKANSNVARYEQEGAPAHVINEFEALLQYHVATYMDNDIAGQP
QALQKSGRPVKSIRARLKGKEGRLRGNLMGKRVDFSARTVITGDPNIDLDQVGVPRSIARTLTYPEVVTPYNIQRLTEFV
RNGPNEHPGAKYIIRDTGERIDLRYHKRAGDIALQYGWKVERHLLDNDPVLFNRQPSLHKMSMMAHRVKVMPYSTFRLNL
SVTSPYNADFDGDEMNLHVPQSEETRAELSQICMVPLQIITPQSNKPIMGIVQDTLCGIRKLTKRDTFCDFDQVMNILFW
IPNWNGIVPQPTIIKPKPLWTGKQMISMCIPDGIHIRRLDKNPLSPEDDGMLIVKGEIMYGVVNKKTIGASAGGLIDVIF
REKGAYACRDFFGNIQKVVNFWLLHNGFSIGIGDTIADTDTMNTITETITSARDEVQQVILDAERNKLEREQGMTLRETF
EQTVSRILNQARDNAGKYAEKSLKELNNVKQMVISGSKGSFINISQMSACVGQQIVEGKRIPFGFADRTLPHFTKDDYSP
ESKGFVENSYLRGLSPQEFFFHAMAGREGLIDTAVKTAETGYIQRRLVKALEDVMVHYDGTVRNSLGDILQFVYGEDGLD
SCKVESQSIDTAPGNNEAFNRRYKIDLMDSSKSLKPSDIESGNDILGDIETQRLLDEEFDLLLKDREFLRHKDFPGDNGL
HLPVNLKRVILNAQQIFNVDPNKATNLTVPEIIDGVKKLTERLIVIRGDDHLTKEAQDNATLLFKAMVRSVLAVKRVVSE
FHLTRDAFEWVLGDIESQFLRSMVNAGEMVGVVAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTP
SMSIFLEREIAIDIERAKDIIRFVQYTNLAQIVKATGIYYDPDPQSTVIEEDKEMVDMFFQVPDENLLSQLDQHSPWVLR
LELDRRKLVDVRLDMNQVAEKISQSFDGDMALIWSDDNAEQLIIRCRIVRDPKSLDEDVAAAEDQKLKMLMFHMLENIVL
CGTPGIQRVYLTKQDIKENDENGDVQIIKAWNLETDGSNLADAMAIEGVDFTRAYCNSFVEVLSVLGIEASRGALFKEIS
NVLSFDGSYVNYRHIALLVDAMTSRGHIMAITRHGINRADTGALMRSSFEETVEILLDAASSAELDDCRGVSENIMLGQI
APLGTGSFEVVLDEEKLISAAPDYTAVGNEARYAGAATGKYMEGAATPYDGRSPMYDNTPQLSGEMAPFSPSVASGAGDD
RGGFTDYEGIQSPFGGSTPGLAPTSPFSYGATSPSYGGATPYSPSAGQYGATSPGYSPTSPSYSPTSPSYSPTSPSYSPT
SPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSFSASGAAPTSPEYSPTSPSFN
AGSSYSPTSPSYTPTSPSFMPNSPMQPGQSYNSGGGMSYSPTSPQYSPSSPRYGGNNNGNNNNNRGNNGNRN

GO term prediction

Biological Process

GO:0006351 transcription, DNA-templated
GO:0006366 transcription from RNA polymerase II promoter

Molecular Function

GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity

Cellular Component

GO:0005665 DNA-directed RNA polymerase II, core complex