Protein

MCA_00081_1

Length
1,489 amino acids

Gene name: SPT6

Description: Transcription elongation factor SPT6

Browser: contigA:209291-213908+

RNA-seq: read pairs 4981, FPKM 41.3, percentile rank 61.0% (100% = highest expression)

Protein function

Annotation:SPT6Transcription elongation factor SPT6
KEGG:K11292SUPT6H transcription elongation factor SPT6
EGGNOG:0PFNWSPT6Plays a role in maintenance of chromatin structure during RNA polymerase II transcription elongation thereby repressing transcription initiation from cryptic promoters. Mediates the reassembly of nucleosomes onto the promoters of at least a selected set of genes during repression
SGD closest match:S000003348SPT6Transcription elongation factor SPT6
CGD closest match:CAL0000194756SPT6Transcription elongation factor SPT6

Protein alignments

%idAln lengthE-value
MIA_04139_164.48%13290.0MIA_04139_1
A0A0J9XH61_GEOCN59.73%13210.0Similar to Saccharomyces cerevisiae YGR116W SPT6 Nucleosome remodeling protein, functions in various aspects of transcription, chromatin maintenance, and RNA processing OS=Geotrichum candidum GN=BN980_GECA17s01561g PE=4 SV=1
A0A170QYV0_9ASCO53.39%13130.0Chromatin-remodeling histone chaperone SPT6 OS=Sugiyamaella lignohabitans GN=SPT6 PE=4 SV=1
UniRef50_A0A170QYV053.39%13130.0Chromatin-remodeling histone chaperone SPT6 n=3 Tax=Saccharomycetales TaxID=4892 RepID=A0A170QYV0_9ASCO
A0A060TA59_BLAAD52.45%13460.0ARAD1D24530p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D24530g PE=4 SV=1
W0TYP5_YARLI44.54%13290.0YALI0B01224p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_B01224g PE=4 SV=1
A0A1E4TBX0_9ASCO42.75%13240.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_28947 PE=4 SV=1
A0A1E3PFS8_9ASCO52.07%9910.0Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_83784 PE=4 SV=1
SPT6_CANAL37.00%13380.0Transcription elongation factor SPT6 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=SPT6 PE=3 SV=1
SPT6_YEAST35.78%12520.0Transcription elongation factor SPT6 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SPT6 PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0023

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
  3. Domain
1 200 400 600 800 1000 1200 1489

Detailed signature matches

  1. Family IPR017072
    Transcription elongation factor Spt6
    1. PIRSF036947 (Spt6)
  2. Domain IPR028083
    Spt6 acidic, N-terminal domain
    1. PF14632 (SPT6_acidic)
  3. Domain IPR028088
    Helix-turn-helix DNA-binding domain of Spt6
    1. PF14641 (HTH_44)
  4. Domain IPR023319
    Tex-like protein, HTH domain
    1. G3DSA:1.10.10...
  5. Domain IPR023323
    Tex-like domain
    1. G3DSA:1.10.35...
  6. Domain IPR012337
    Ribonuclease H-like domain
    1. SSF53098 (Ribonucle...)
  7. Domain IPR006641
    YqgF/RNase H-like domain
    1. G3DSA:3.30.42...
  8. Domain IPR028231
    Transcription elongation factor Spt6, YqgF domain
    1. PF14639 (YqgF)
  9. Domain IPR032706
    Transcription elongation factor Spt6, helix-hairpin-helix motif
    1. PF14635 (HHH_7)
  10. Domain IPR010994
    RuvA domain 2-like
    1. SSF47781 (RuvA doma...)
  11. Domain IPR027999
    Death-like domain of Spt6
    1. PF14878 (DLD)
  12. Domain IPR035420
    Spt6, SH2 domain
    1. PF14633 (SH2_2)
  13. Domain IPR000980
    SH2 domain
    1. SM00252 (SH2_5)
    2. PS50001 (SH2)
    3. SSF55550 (SH2 domain)
    4. G3DSA:3.30.50...
  14. Domain IPR035019
    Spt6, SH2 domain, N terminus
    1. cd09918 (SH2_Nterm_...)
  15. Domain IPR035018
    Spt6, SH2 domain, C terminus
    1. cd09928 (SH2_Cterm_...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:1.10.15...
  3. G3DSA:2.40.50...
  4. G3DSA:3.40.63...
  5. SSF158832 (Tex N-te...)
  6. mobidb-lite (disord...)

Residue annotation

  1. phosphotyrosine bi...
  2. hydrophobic bindin...
  3. phosphotyrosine bi...
  4. hydrophobic bindin...

Protein sequence

>MCA_00081_1
MSEEYDEDSYAEVRDDENDDEENPKSDIDRDSSEEEEDDDEEELQKIKDGFIVDDEDEEEEEDEDDVSRHRKRRKKKRRH
RTHSDDEDNEHSRNHDDDLDEDDLALLMENTGRQIEKPSSSRQFKRLKRANQDNDSGRGGLNDMFSDEAEEDDLGYEAQS
PQQSQPSRRTFASEFDDFIEDDEFSDDQNMEESQPSRRSEKLTRGPEALALQYKGLDEDKLQELYEVFGDGEEYEWALEG
EDDLEQMGYSSEDGEKAGPDLKDVFEPGELKNKLLTDEDNAIRAKDVPERYQLLRANFKDDYDLTDEEFEQEVQWITQKL
SQEKSKLFAAKPHLRAPFKKAVQSVVEFICKENLEVPFIWLHRKDFLVYVRPNAEGQSSSSGPVTDLLLTIDNLWRIVHL
DIEFHGILLKSKACEKLYNSLGIYDDTYLEMFRNASSFSDYQDLFDYLHFNYSSKIKENINSTAEKSRGTYKRPSHFSRF
DRIRNGPIYPFLKSYGITAQNFGANVSSAARIYFTEDHPTAPLELAEQYVGTTPEGKPSAYKSAQHVLDDAQAMFAEEIY
YEPRVRKFLRDAFWKNAKVDIIVTEKGRKQIDEESPYADIKYAINRSFDDLRLRPELYLRMLNAENEGLVIVRISYPNYK
NTLFETLFSKYLASDNVSDIAKAWNESRRAAFKATSQKIIPHICGNIKEDLRTECIRSLYYEIREQFAKRLDQAPFKPYG
YAAGTTPRVLTLSWGSGQSGKDAVLATVLNEEGKLIEVAKLEDPRNPAFKTNFVALVRKRVPDVVGVAGFTVSSKQLFQE
IADIIKAEDLTSGSDVESTVPLDLIWVQDEVAHLFKNTQRAIEEFPDQVTLSRYCIALARYVQSPLLEYASLEDMTSIRV
HPYQRYLPVETFNEAIETVFVDYVNMVGVDINEAIRNPYIANVVKYVAGLGPRKASSILHGIQSHGGSLTNRAELVTNNI
TGKSIFMNCSSFFIIPYNERTISNEATDILDATRIHPQDYYIARKMATDALELDEEDVEAYDSQGGVVAQLINEDVEKLN
ELILEEYANELEREYNKKKRETLEMIKSEIQRHFGEKRSPFHILTEMEVFTMLTGETKDTLHNGVIVPFNIKRVNNQFLS
GVLSCGVEGTVSLADMFDPGAEAPHPSTAFSILQTVQTVILDIDYSKFVAKLSTAESRIKEAREQRKKTQRKDPNYWNIA
AEEAEKMRLAQKKEEERRATRVIKHPLFKPFNSRQAEDYLATMSRGDCVIRPSSRGDDHIAITWKVADGIFQHVDVLELD
KPNQFSIGNVLQVGNLRFSDLDELIITYIQAIARKIQQMVNHEKFFQKPKSEVEEWLKAYTRSNPKRSVYAFCFDHKRAG
YISLMFQTGQGSPIQTWSVKVIPGGYSLLKHEYPDVISLINGFKQIMKQLSTQNQQQQHRAPPPSHNNNNYYRNNGGPPP
PMYSNDGRGGGRGYPPSGPPGGYRDRERDRYPGGRDPGRDPRREVYYRR

GO term prediction

Biological Process

GO:0006139 nucleobase-containing compound metabolic process
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0032784 regulation of DNA-templated transcription, elongation

Molecular Function

GO:0000991 transcription factor activity, core RNA polymerase II binding
GO:0003676 nucleic acid binding
GO:0003677 DNA binding

Cellular Component

None predicted.