Protein

MIA_03644_1

Length
474 amino acids

Browser: contig04:1782775-1784362+

Protein function

EGGNOG:0PJIRTEF1This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis
SGD closest match:S000006284TEF1Elongation factor 1-alpha
CGD closest match:CAL0000196876TEF2Elongation factor 1-alpha 2

Protein alignments

%idAln lengthE-value
MCA_04441_286.456%4430.0MCA_04441_2
EF1A2_CANAL86.907%4430.0Elongation factor 1-alpha 2 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=TEF2 PE=3 SV=2
UniRef50_P0CY3586.907%4430.0Elongation factor 1-alpha 1 n=2464 Tax=cellular organisms TaxID=131567 RepID=EF1A1_CANAL
EF1A_YEAST85.779%4430.0Elongation factor 1-alpha OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TEF1 PE=1 SV=1
A0A060T453_BLAAD85.553%4430.0Elongation factor 1-alpha OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C01892g PE=3 SV=1
A0A1E4TA38_9ASCO85.520%4420.0Elongation factor 1-alpha OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_52543 PE=3 SV=1
EF1A_YARLI86.486%4440.0Elongation factor 1-alpha OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=TEF PE=2 SV=2
A0A167DIZ3_9ASCO81.525%3410.0Translation elongation factor EF-1 alpha OS=Sugiyamaella lignohabitans GN=TEF1 PE=4 SV=1
I3Y136_GEOCN82.119%3027.92e-179Translation elongation factor 1-alpha (Fragment) OS=Geotrichum candidum PE=4 SV=1
A0A1E3PL22_9ASCO84.861%2518.95e-150Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_41536 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0724

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 50 100 150 200 250 300 350 400 474

Detailed signature matches

  1. Family IPR004539
    Translation elongation factor EF1A, eukaryotic/archaeal
    1. TIGR00483
    2. MF_00118_A (EF_Tu_A)
  2. Domain IPR027417
    P-loop containing nucleoside triphosphate hydrolase
    1. SSF52540 (P-loop co...)
  3. Domain IPR000795
    Transcription factor, GTP-binding domain
    1. PS51722 (G_TR_2)
    2. PF00009 (GTP_EFTU)
    3. PR00315 (ELONGATNFCT)
  4. Domain IPR009000
    Translation protein, beta-barrel domain
    1. SSF50447 (Translati...)
  5. Domain IPR004161
    Translation elongation factor EFTu-like, domain 2
    1. PF03144 (GTP_EFTU_D2)
  6. Domain IPR009001
    Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal
    1. SSF50465 (EF-Tu/eEF...)
  7. Domain IPR004160
    Translation elongation factor EFTu/EF1A, C-terminal
    1. PF03143 (GTP_EFTU_D3)
  8. SITE IPR031157
    Tr-type G domain, conserved site
    1. PS00301 (G_TR_1)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:2.40.30.10
  2. G3DSA:3.40.50...
  3. cd01883 (EF1_alpha)
  4. cd03693 (EF1_alpha_II)
  5. cd03705 (EF1_alpha_III)

Residue annotation

  1. G1 box cd01883
  2. putative GEF inter...
  3. GTP/Mg2+ binding s...
  4. EF1Balpha binding ...
  5. Switch I region cd...
  6. G2 box cd01883
  7. G3 box cd01883
  8. Switch II region c...
  9. G4 box cd01883
  10. G5 box cd01883
  11. EF1 beta interface...
  12. pelota interface c...
  13. 18S rRNA binding s...
  14. pelota interface c...
  15. heterodimer interf...

Protein sequence

>MIA_03644_1
MVTQLGLWHLSEYLLKMGKEKTHVNIVVIGHVDSGKSTTTGHLIFKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLK
AERERGITIDIALWKFETPKYYVTVIDAPGHRDFIKNMITGTSQADCAILIIAAGVGEFEAGISKDGQTKEHVLLAFTLG
VRQLIVAVNKMDSIKWDQNRFKDIVAEASKFTKKAGYKPDEIPFIPISGWNGDNMIEPTTNAPWYKGWTKKTKTGEITGK
TLLEAIDAIVPPVRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPANVTTEVKSVEMHHEQLAEGLPGD
NVGFNVKNVSVKDIRRGNVCGDSKNDPPKACASFNAQVIVLNHPGQIAKGYAPVLDCHTAHIACKFDELIDKIDKRTGKS
TEKEPKFIKSGDAAIVKLVPSKPMCVETYTEYPPLGRFAVRDMRQTVAVGVIKSVEKVDAAGKVTKSAAKAAKK

GO term prediction

Biological Process

GO:0006414 translational elongation

Molecular Function

GO:0003746 translation elongation factor activity
GO:0003924 GTPase activity
GO:0005525 GTP binding

Cellular Component

GO:0005737 cytoplasm