Protein

MIA_03518_1

Length
1,190 amino acids

Browser: contig04:1423222-1426846+

Protein function

EGGNOG:0PFUXPIM1ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial DNA in a site-specific manner (By similarity)
SGD closest match:S000000118PIM1Lon protease homolog, mitochondrial
CGD closest match:CAL0000197537PIM1Lon protease homolog, mitochondrial

Protein alignments

%idAln lengthE-value
MCA_02745_176.994%9780.0MCA_02745_1
A0A0J9X4P2_GEOCN69.619%9710.0Lon protease homolog, mitochondrial OS=Geotrichum candidum GN=PIM1 PE=3 SV=1
UniRef50_A0A0J9XEH258.624%11770.0Lon protease homolog, mitochondrial n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XEH2_GEOCN
LONM_YEAST55.714%9800.0Lon protease homolog, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PIM1 PE=1 SV=2
A0A1E4TA13_9ASCO54.592%9690.0Lon protease homolog, mitochondrial OS=Tortispora caseinolytica NRRL Y-17796 GN=PIM1 PE=3 SV=1
LONM_YARLI46.964%9880.0Lon protease homolog, mitochondrial OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=PIM1 PE=3 SV=1
A0A1E3PFX5_9ASCO50.946%7930.0Lon protease homolog OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_47538 PE=3 SV=1
LONM_CANAL40.273%9510.0Lon protease homolog, mitochondrial OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PIM1 PE=3 SV=1
A0A060T362_BLAAD50.000%6540.0Lon protease homolog OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C29788g PE=3 SV=1
A0A167EV24_9ASCO46.484%5831.52e-167Lon protease homolog OS=Sugiyamaella lignohabitans GN=PIM1 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9814
Predicted cleavage: 30

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 200 400 600 800 1000 1190

Detailed signature matches

  1. Family IPR004815
    Lon protease, bacterial/eukaryotic-type
    1. TIGR00763
  2. Family IPR027503
    Lon protease homologue, chloroplastic/mitochondrial
    1. MF_03120 (lonm_euk)
  3. Domain IPR015947
    PUA-like domain
    1. SSF88697 (PUA domai...)
  4. Domain IPR003111
    Lon, substrate-binding domain
    1. PF02190 (LON_substr...)
    2. SM00464 (lon_5)
    3. PS51787 (LON_N)
  5. Domain IPR027417
    P-loop containing nucleoside triphosphate hydrolase
    1. SSF52540 (P-loop co...)
  6. Domain IPR003593
    AAA+ ATPase domain
    1. SM00382 (AAA_5)
  7. Domain IPR003959
    ATPase, AAA-type, core
    1. PF00004 (AAA)
  8. Domain IPR020568
    Ribosomal protein S5 domain 2-type fold
    1. SSF54211 (Ribosomal...)
  9. Domain IPR014721
    Ribosomal protein S5 domain 2-type fold, subgroup
    1. G3DSA:3.30.23...
  10. Domain IPR008269
    Peptidase S16, Lon proteolytic domain
    1. PF05362 (Lon_C)
    2. PS51786 (LON_PROTEO...)
  11. SITE IPR008268
    Peptidase S16, active site
    1. PS01046 (LON_SER)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:1.10.40...
  2. G3DSA:1.10.8.60
  3. G3DSA:2.30.13...
  4. G3DSA:3.40.50...
  5. PR00830 (ENDOLAPTASE)
  6. cd00009 (AAA)
  7. mobidb-lite (disord...)

Residue annotation

  1. Walker A motif cd0...
  2. ATP binding site c...
  3. Walker B motif cd0...
  4. arginine finger cd...

Protein sequence

>MIA_03518_1
MLSSSQVLKRSGYQQTLRVVSCVRTQLRSASHSSKADASIARILAEKKLNNRAQDVPLHLLPNSESKTPSSLSSLVNTPF
FSSLNNQNDLKILANSDNNDNSPPKGPSTDTPTKPKRERKKNDDNSSEKSKDEKTPAADGKEPSNDTVDVGHSSSATKNS
KSSESSKKKDSNKSSSNNKPDNAAPVPPASSEPTHHRRPINATDPTRTNTEALFSVASAAEKKQVLILPINRRPLIPGFH
KAVDVKDKNVIKALKKILKEDLDDKCIGVFLLKEEKADTDVINSLDEVYPVGVYARIGGSFPGKSPDGGETFILYPTHRI
QIGDLVDAKTGNGETAQVSTASVSELPSEGELEHESEVEAEPVSSTPDSDVTSFEKDTISEDDKAHHELYNIPASWSKNM
VDLEDLPYDHHSDMIKALTGEILKILKELSVLNRPLKDQVVNLVLAGNKFSSSNVYEEPHILADFAASVSAGRASEIQDV
LACLDIEERLQKALVLIKRELINIQLQEKISADVDNKIQKRQREYFLMEQLKGIKRELGMDDGRERIIQTFRERAEKLIM
PESVSKVFEDEINKLMTLEPNAAEYNVTRQYLDWITQVPWGKYSKDSYNIKNAMKILDEDHYGLKDVKDRILEFIAVGKL
LGTVDGKIICFVGPPGVGKTSVAKSIAKSLNRKFYRFSIGGVSDVSELKGHRRTYVGALPGRIVQALKKTQTQNPLILID
EIDKIGRAGYHGDPSAALLELLDPEQNSSFLDHYMDVPIDMSKTLFVCTANSLDTIPGPLLDRMEVIEISGYVAEEKIAI
AERYLAPQAKKLAGLEDAHVTLTHEAIQGIISKYARESGVRNLKKQIEKIYRKAALNIIKDVGEDNFVEENVEEQPEVES
ASTSIKTESTGEATSESEPVVTPESETKEQTTAETNSDKVIVDEEPQKISQHKPLKVPDSISIEIAPSDLKDYIGPPLYT
EDRMYEKTPIGVVMGLGVTSMGGSTLYVESVLEQPLTIDSHAGMSQTGQLGSTMKESSSIAYSFSRMFIGKNFPENRFFE
RAKIHLHCPEGAIPKDGPSAGITMATSLLSLALNEALPPTIAMTGELTLTGKVLRIGGLREKSVAAKRSGATTIFFPKDN
LADWEAMPDNIKEGLEPVPVAWYSEVFERAFSNLDHKAANQLWKTQFEKIDIAKHDISSPSSSATVSATA

GO term prediction

Biological Process

GO:0006508 proteolysis
GO:0006515 misfolded or incompletely synthesized protein catabolic process

Molecular Function

GO:0004176 ATP-dependent peptidase activity
GO:0004252 serine-type endopeptidase activity
GO:0005524 ATP binding

Cellular Component

None predicted.