Protein

MIA_03406_1

Length
562 amino acids

Browser: contig04:1122620-1124309+

Protein function

EGGNOG:0PFWQPET112Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) (By similarity)
SGD closest match:S000000176PET112Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
CGD closest match:CAL0000189636PET112Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial

Protein alignments

%idAln lengthE-value
MCA_04526_164.954%5450.0MCA_04526_1
A0A0J9XHV6_GEOCN58.364%5380.0Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial OS=Geotrichum candidum GN=PET112 PE=3 SV=1
A0A060T4I7_BLAAD52.135%5625.94e-174Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial OS=Blastobotrys adeninivorans GN=PET112 PE=3 SV=1
UniRef50_A0A060T4I752.135%5621.47e-170Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial n=1 Tax=Blastobotrys adeninivorans TaxID=409370 RepID=A0A060T4I7_BLAAD
A0A1E3PFY8_9ASCO47.091%5508.43e-155Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial OS=Nadsonia fulvescens var. elongata DSM 6958 GN=PET112 PE=3 SV=1
GATB_YARLI42.549%5573.00e-123Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=PET112 PE=3 SV=1
A0A1E4TIC0_9ASCO42.430%5351.58e-120Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_14594 PE=3 SV=1
GATB_CANAL37.570%5355.45e-119Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PET112 PE=3 SV=2
GATB_YEAST37.276%5584.55e-114Glutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PET112 PE=1 SV=3
A0A167EKY9_9ASCO44.940%3364.59e-81Glutamyl-tRNA(Gln) amidotransferase subunit PET112 OS=Sugiyamaella lignohabitans GN=PET112 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.6113
Predicted cleavage: 18

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 562

Detailed signature matches

  1. Family IPR004413
    Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit
    1. MF_00121 (GatB)
    2. TIGR00133
  2. Domain IPR006075
    Aspartyl/Glutamyl-tRNA(Gln) amidotransferase, subunit B/E, catalytic
    1. PF02934 (GatB_N)
  3. Domain IPR003789
    Aspartyl/glutamyl-tRNA amidotransferase subunit B-related
    1. SSF89095 (GatB/YqeY...)
  4. Domain IPR018027
    Asn/Gln amidotransferase
    1. SM00845 (gatb_yqey_2)
    2. PF02637 (GatB_Yqey)
  5. Domain IPR023168
    Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B, C-terminal
    1. G3DSA:1.10.10...
Unintegrated signatures no IPR
Unintegrated signatures
  1. SSF55931 (Glutamine...)

Protein sequence

>MIA_03406_1
MLRYQQLRQFASAASKSPKIDPSKYKLTCGLEIHAQLMTPQKLFSAQNTGSLHAPPNSQVSFYDAALPGTQPLLNPNNVF
LALRAAVALGCSVAPRFSFDRKHYFYGDQPAGYQITQHFEPYATDGLLTLYARDLSDGKKRAAAGAIKDVNVRIKQIQIE
QDTGKSMYIDDTANIDLNRTNHCLIEMVTEPDIPTPEAAGIFVRKLQQLLAHLGVCTGELESGAMRVDVNVSVSRRTVED
TWELGERCEIKNLSTTSAVVHAIRAEFSRQVADVEAGREIERETRGWDGRRTWKLRSKEDGVDYRYMPDPEVLPVELEPD
VVERVTAGLPELPDAILQRLLAAPYNVPLRDARTLMAGGEEKEDTSGEVPQRVLGQAGTLPVVEYYEAVFARLAASGAET
RLAANWIVHTLLGELKTLGRTAFVEGQTVPSAAQFGDLLAAVSSKELTAASGKLVLKHLLQNPQATATRSIEEIVEEFEL
ARAPMGGSAPGGGAGSEDDVAAALREVCSEIMAKSPKIVEQITSGKKPKSIQFLIGQVMRETQGRVDPQAIEVVFKELLG
LK

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0016874 ligase activity
GO:0016884 carbon-nitrogen ligase activity, with glutamine as amido-N-donor

Cellular Component

None predicted.