Protein

MIA_03314_1

Length
562 amino acids

Browser: contig04:843957-845714-

Protein function

EGGNOG:0PG1IROK1ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA
SGD closest match:S000003139ROK1ATP-dependent RNA helicase ROK1
CGD closest match:CAL0000178978CHR1ATP-dependent RNA helicase CHR1

Protein alignments

%idAln lengthE-value
A0A0J9X726_GEOCN65.406%5290.0Similar to Saccharomyces cerevisiae YGL171W ROK1 ATP-dependent RNA helicase of the DEAD box family OS=Geotrichum candidum GN=BN980_GECA04s02078g PE=3 SV=1
MCA_06331_167.619%5250.0MCA_06331_1
A0A161HKX2_9ASCO59.404%5370.0RNA-dependent ATPase ROK1 OS=Sugiyamaella lignohabitans GN=ROK1 PE=3 SV=1
A0A1E3PK54_9ASCO56.848%5330.0ATP-dependent RNA helicase ROK1 OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_50858 PE=3 SV=1
A0A060T367_BLAAD57.037%5400.0ARAD1A07282p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A07282g PE=3 SV=1
ROK1_YEAST51.692%5323.79e-175ATP-dependent RNA helicase ROK1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ROK1 PE=1 SV=1
UniRef50_P4581851.692%5329.08e-172ATP-dependent RNA helicase ROK1 n=95 Tax=Saccharomycetales TaxID=4892 RepID=ROK1_YEAST
ROK1_CANAL48.333%5401.58e-167ATP-dependent RNA helicase CHR1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=CHR1 PE=3 SV=2
ROK1_YARLI49.251%5343.59e-157ATP-dependent RNA helicase ROK1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=ROK1 PE=3 SV=1
A0A1E4TDH7_9ASCO58.633%2783.55e-105Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_32953 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.2035
Predicted cleavage: 11

Protein family membership

None predicted.

Domains and repeats

  1. Domain
  2. Domain
  3. Domain
1 100 200 300 400 500 562

Detailed signature matches

  1. Domain IPR014014
    RNA helicase, DEAD-box type, Q motif
    1. PS51195 (Q_MOTIF)
  2. Domain IPR014001
    Helicase superfamily 1/2, ATP-binding domain
    1. PS51192 (HELICASE_A...)
    2. SM00487 (ultradead3)
  3. Domain IPR011545
    DEAD/DEAH box helicase domain
    1. PF00270 (DEAD)
  4. Domain IPR027417
    P-loop containing nucleoside triphosphate hydrolase
    1. SSF52540 (P-loop co...)
  5. Domain IPR001650
    Helicase, C-terminal
    1. PS51194 (HELICASE_CTER)
    2. SM00490 (helicmild6)
    3. PF00271 (Helicase_C)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:3.40.50...
  3. cd00079 (HELICc)
  4. mobidb-lite (disord...)

Residue annotation

  1. nucleotide binding...
  2. ATP-binding site c...

Protein sequence

>MIA_03314_1
MDMFNLLSRGASINRRGKDAKTFDVLMGKSATSNVAEEKKKRADLARTLDFFNETPAQEVQDKKSDLDENEDKNNSDDSD
SEEKNAPLVISTKQQAEEQRQRLDTKVKGDDLPLPIGSFQDLRTRFDLHKHLLRNLERQNFQRPTPIQAEAMPILLHGRD
LVACAPTGSGKTLAFAVPIVQALKQHEGGGIRCLVVTPTKELATQIYGQFVKLSQGRDLHICVLNKAQAAKFANETNKRK
FDIMISTPLRLLALTNQGKLDLSRVKHVVLDEVDKLFEQGFIQQTDSILEQCTAPDVQRAMFSATVPSGVEEIASNIMVS
PVRVIVGKKEGANTQIEQRIVYTGSEHGKLIEIRRMLQEGELEPPVIVFLQSVARAKALYHELIYDGVNVDVIHGERTQR
QRDRVIERFKNGDIWVLICTDVLARGIDFRGVSLVINYDVPESAQAYVHRIGRTGRAGRTGKAITFYTKEDAVGIKAVVN
VMKQSGSEVPEWMLGMRKATNDEKKKLRFKPVERDEISTVAKRKRENNNKNQKKSGSKGGKSEGTEEPKKKKKVDEEEED
EK

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003676 nucleic acid binding
GO:0005524 ATP binding

Cellular Component

None predicted.