MIA_03254_1

Protein

Length

1,049 amino acids

Protein function

EGGNOG:	0PGGX	PIK1	phosphatidylinositol 4-kinase
SGD closest match:	S000005211	PIK1	Phosphatidylinositol 4-kinase PIK1
CGD closest match:	CAL0000190377	PIKA	Phosphatidylinositol 4-kinase PIK1a

Protein alignments

	%id	Aln length	E-value
MCA_03881_1	64.862%	1016	0.0	MCA_03881_1
A0A0J9X3H8_GEOCN	52.913%	1030	0.0	Similar to Saccharomyces cerevisiae YNL267W PIK1 Phosphatidylinositol 4-kinase OS=Geotrichum candidum GN=BN980_GECA01s03530g PE=3 SV=1
UniRef50_A0A0J9X3H8	52.913%	1030	0.0	Similar to Saccharomyces cerevisiae YNL267W PIK1 Phosphatidylinositol 4-kinase n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9X3H8_GEOCN
Q6CFY9_YARLI	44.669%	741	0.0	YALI0B02376p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_B02376g PE=3 SV=1
A0A060T8R9_BLAAD	59.479%	422	4.08e-165	ARAD1D09504p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D09504g PE=3 SV=1
A0A167DDQ9_9ASCO	57.957%	421	2.60e-158	1-phosphatidylinositol 4-kinase OS=Sugiyamaella lignohabitans GN=PIK1 PE=3 SV=1
A0A1E3PIH6_9ASCO	55.869%	426	1.52e-150	Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_83264 PE=3 SV=1
A0A1E4TL39_9ASCO	51.300%	423	9.26e-141	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_87083 PE=3 SV=1
PIK1_YEAST	50.591%	423	4.48e-129	Phosphatidylinositol 4-kinase PIK1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PIK1 PE=1 SV=1
PIK1A_CANAL	46.189%	433	8.27e-112	Phosphatidylinositol 4-kinase PIK1a OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PIKA PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0387

Protein family membership

Phosphatidylinositol 4-kinase, Pik1, fungi (IPR021601)

Domains and repeats

Domain
1 - 147
Armadillo-type fold (IPR016024)
Phosphoinositide 3-kinase, accessory (PIK) domain (IPR001263)
330 - 446
Protein kinase-like domain (IPR011009)
768 - 1,046
Protein kinase-like domain (IPR011009)
Phosphatidylinositol 3-/4-kinase, catalytic domain (IPR000403)

1 100 200 300 400 500 600 700 800 900 1049

Detailed signature matches

IPR021601

Phosphatidylinositol 4-kinase, Pik1, fungi
1. PF11522 (Pik1)
  
  136 - 181
  
  Pfam
  PF11522 (Pik1)
IPR016024

Armadillo-type fold
1. SSF48371 (ARM repeat)
  
  45 - 127
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
IPR001263

Phosphoinositide 3-kinase, accessory (PIK) domain
1. PS51545 (PIK_HELICAL)
  
  1 - 147
  
  PROSITE profiles
  PS51545 (PIK_HELICAL)
IPR011009

Protein kinase-like domain
1. SSF56112 (Protein k...)
  
  768 - 1,041
  
  SUPERFAMILY
  SSF56112 (Protein kinase-like (PK-like))
  
  330 - 446
  
  SUPERFAMILY
  SSF56112 (Protein kinase-like (PK-like))
IPR000403

Phosphatidylinositol 3-/4-kinase, catalytic domain
1. PS50290 (PI3_4_KINA...)
  
  776 - 1,024
  
  PROSITE profiles
  PS50290 (PI3_4_KINASE_3)
2. SM00146 (pi3k_hr1_6)
  
  777 - 1,046
  
  SMART
  SM00146 (pi3k_hr1_6)
3. PF00454 (PI3_PI4_ki...)
  
  777 - 987
  
  Pfam
  PF00454 (PI3_PI4_kinase)
4. G3DSA:1.10.10...
  
  832 - 1,032
  
  GENE3D
  G3DSA:1.10.1070.11 (G3DSA:1.10.1070.11)
IPR018936

Phosphatidylinositol 3/4-kinase, conserved site
1. PS00916 (PI3_4_KINA...)
  
  886 - 906
  
  PROSITE patterns
  PS00916 (PI3_4_KINASE_2)

no IPR

Unintegrated signatures

Coil

631 - 651

COILS
Coil (Coil)
G3DSA:1.25.40.70

24 - 153

GENE3D
G3DSA:1.25.40.70 (G3DSA:1.25.40.70)
G3DSA:3.30.10...

326 - 831

GENE3D
G3DSA:3.30.1010.10 (G3DSA:3.30.1010.10)
cd05168 (PI4Kc_III_...)

750 - 1,048

CDD
cd05168 (PI4Kc_III_beta)
mobidb-lite (disord...)

506 - 533

MobiDB Lite
mobidb-lite (disorder_prediction)

1 - 22

MobiDB Lite
mobidb-lite (disorder_prediction)

218 - 269

MobiDB Lite
mobidb-lite (disorder_prediction)

706 - 751

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

ATP binding site c...

759E

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

761I

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

762R

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

764A

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

766P

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

779I

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

781K

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

784S

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

815M

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

827V

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

828E

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

829T

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

830I

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

835S

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

838S

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

906N

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

908L

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

918I

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)

919D

ATP binding site
759E, 761I, 762R, 764A, 766P, 779I, 781K, 784S, 815M, 827V, 828E, 829T, 830I, 835S, 838S, 906N, 908L, 918I, 919D
CDD
cd05168 (PI4Kc_III_beta)
catalytic loop cd0...

898Q

catalytic loop
898Q, 899I, 900K, 901D, 902R, 903H, 904N, 905G, 906N
CDD
cd05168 (PI4Kc_III_beta)

899I

catalytic loop
898Q, 899I, 900K, 901D, 902R, 903H, 904N, 905G, 906N
CDD
cd05168 (PI4Kc_III_beta)

900K

catalytic loop
898Q, 899I, 900K, 901D, 902R, 903H, 904N, 905G, 906N
CDD
cd05168 (PI4Kc_III_beta)

901D

catalytic loop
898Q, 899I, 900K, 901D, 902R, 903H, 904N, 905G, 906N
CDD
cd05168 (PI4Kc_III_beta)

902R

catalytic loop
898Q, 899I, 900K, 901D, 902R, 903H, 904N, 905G, 906N
CDD
cd05168 (PI4Kc_III_beta)

903H

catalytic loop
898Q, 899I, 900K, 901D, 902R, 903H, 904N, 905G, 906N
CDD
cd05168 (PI4Kc_III_beta)

904N

catalytic loop
898Q, 899I, 900K, 901D, 902R, 903H, 904N, 905G, 906N
CDD
cd05168 (PI4Kc_III_beta)

905G

catalytic loop
898Q, 899I, 900K, 901D, 902R, 903H, 904N, 905G, 906N
CDD
cd05168 (PI4Kc_III_beta)

906N

catalytic loop
898Q, 899I, 900K, 901D, 902R, 903H, 904N, 905G, 906N
CDD
cd05168 (PI4Kc_III_beta)
activation loop (A...

919D

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

920F

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

921G

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

922F

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

923L

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

924L

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

925S

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

926N

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

927S

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

928P

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

929G

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

930S

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

931V

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

932G

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

933F

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

934E

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

936S

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

937P

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

938F

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

939K

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

940L

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

941T

activation loop (A-loop)
919D, 920F, 921G, 922F, 923L, 924L, 925S, 926N, 927S, 928P, 929G, 930S, 931V, 932G, 933F, 934E, 936S, 937P, 938F, 939K, 940L, 941T
CDD
cd05168 (PI4Kc_III_beta)

Protein sequence

>MIA_03254_1
MSLQEPSSNPQPPTAPPSTRLTHSPIAVSTAGGYSFLLRFIDSPQFTLFHCISYLERYADEIGIHFYLAQKLRNYPDEEI
EFFVPQIVQLHVTIKTDSLALEDLIIELCSRSTHSAVLIFWQVQAHLTELSDEPESHGFIVCQRLMAKLQHVLFNVGLPP
DDKINENTLPALVLAGSLAAGLGLPGAVNYIKPLVVSQGRKKRSLIFQLAHKAGSAVFSPTSSSFRRSASASSPSSPSKS
SPPPNLTRHTSLSTSQLNPGSKPYSAHYDVNSTSPDLSLPAYLNYRHDIYHNKIPTVQSTLEGSFAESEISILSPSYQVQ
LRNVNVLKSNYFYCETQFMDALHKISNKLCQVPKQARLSALRVEIAMLNKDLPAEVDIPLLLPNAVSSTASARSLTSSSS
SATFPHDFHLGDLPKQNRLIRIVPSEAVLLNSAERVPYLLLIEYIRNDVDFNPGSDKNRAILNQEVNRRHIFDTLSVPVL
GDFQHSFSAPSSTRNSEDVNMFSTVNPQSRLSRQRTPSTWSLPSSSSKTSRNNRLALSEIIPKEESDMGDISVIDLFDDN
KRTMSQLSASLSSLASLGSSYFSYGKNTNGSNASSTNNALNSGFTSSPRSSDLSFSPAYMTPLQTSQTDEIDELAARMRT
AAVMINQLESAGGAKLSKDEVERIKNRIILSMQSMEEHTVYQSNNVLGAAGERKLENDLIIAGIDDDSSDRAGSSGSTSS
SNNGGARLKRHSRNLSTASMSTSSKTPLSLGEDWKAKKERIRRASPYGHFPGWDLFSCIVKTGSDLRQEAFACQLIQAMQ
MCWERNNTGVWVKRMRILITHNSAGLVETITNALSIHSIKKAMSTTLENSSSGGKSVIPSLKHYFETTFGAATPQYEQAV
DNFVRSLAAYSVICYLLQIKDRHNGNILLDNVGHIVHIDFGFLLSNSPGSVGFESSPFKLTHEYVDLMGGADSETFKLFK
SLVRQAFKDIRKEAESIIILVEMMQRDSTLPCFASGAATANQLRQRFQLQMSDSEVDSYVDHMLIQKSFGSFSTRLYDQY
QLLTQGIYS

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0005488 binding
GO:0016301 kinase activity
GO:0016773 phosphotransferase activity, alcohol group as acceptor

Cellular Component