MIA_03024_1

Protein

Length

308 amino acids

Protein function

EGGNOG:	0PKGR	FG03667.1	Catechol dioxygenase
CGD closest match:	CAL0000188195	HQD2	Catechol 1,2-dioxygenase

Protein alignments

	%id	Aln length	E-value
MCA_00701_1	82.68%	306	0.0	MCA_00701_1
A0A167DF12_9ASCO	69.58%	309	6e-156	Uncharacterized protein OS=Sugiyamaella lignohabitans GN=AWJ20_1116 PE=4 SV=1
HQD2_CANAL	55.52%	299	3e-123	Catechol 1,2-dioxygenase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=HQD2 PE=1 SV=1
UniRef50_P86029	55.52%	299	7e-120	Catechol 1,2-dioxygenase n=25 Tax=saccharomyceta TaxID=716545 RepID=HQD2_CANAL
A0A060T9I8_BLAAD	38.74%	253	7e-59	ARAD1D18458p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D18458g PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0655

Protein family membership

None predicted.

Domains and repeats

Domain
1 - 296
Intradiol ring-cleavage dioxygenase, core (IPR015889)
Domain
19 - 89
Catechol dioxygenase, N-terminal (IPR007535)
97 - 272
Intradiol ring-cleavage dioxygenase, C-terminal (IPR000627)

1 50 100 150 200 250 308

Detailed signature matches

IPR015889

Intradiol ring-cleavage dioxygenase, core
1. SSF49482 (Aromatic ...)
  
  2 - 261
  
  SUPERFAMILY
  SSF49482 (Aromatic compound dioxygenase)
2. G3DSA:2.60.13...
  
  1 - 296
  
  GENE3D
  G3DSA:2.60.130.10 (G3DSA:2.60.130.10)
IPR007535

Catechol dioxygenase, N-terminal
1. PF04444 (Dioxygenase_N)
  
  19 - 89
  
  Pfam
  PF04444 (Dioxygenase_N)
IPR000627

Intradiol ring-cleavage dioxygenase, C-terminal
1. PS00083 (INTRADIOL_...)
  
  127 - 155
  
  PROSITE patterns
  PS00083 (INTRADIOL_DIOXYGENAS)
2. PF00775 (Dioxygenase_C)
  
  97 - 272
  
  Pfam
  PF00775 (Dioxygenase_C)

no IPR

Unintegrated signatures

Coil

284 - 304

COILS
Coil (Coil)
cd03461 (1,2-HQD)

3 - 277

CDD
cd03461 (1,2-HQD)

Residue annotation

dimer interface cd...

20K

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

22R

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

24I

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

26G

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

27S

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

33H

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

37R

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

38E

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

39N

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

45E

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

46W

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

71S

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

72D

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

79L

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

84T

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)

206R

dimer interface
20K, 22R, 24I, 26G, 27S, 33H, 37R, 38E, 39N, 45E, 46W, 71S, 72D, 79L, 84T, 206R
CDD
cd03461 (1,2-HQD)
active site cd03461

72D

active site
72D, 100P, 101F, 155Y, 189Y, 210R, 213H, 215H
CDD
cd03461 (1,2-HQD)

100P

active site
72D, 100P, 101F, 155Y, 189Y, 210R, 213H, 215H
CDD
cd03461 (1,2-HQD)

101F

active site
72D, 100P, 101F, 155Y, 189Y, 210R, 213H, 215H
CDD
cd03461 (1,2-HQD)

155Y

active site
72D, 100P, 101F, 155Y, 189Y, 210R, 213H, 215H
CDD
cd03461 (1,2-HQD)

189Y

active site
72D, 100P, 101F, 155Y, 189Y, 210R, 213H, 215H
CDD
cd03461 (1,2-HQD)

210R

active site
72D, 100P, 101F, 155Y, 189Y, 210R, 213H, 215H
CDD
cd03461 (1,2-HQD)

213H

active site
72D, 100P, 101F, 155Y, 189Y, 210R, 213H, 215H
CDD
cd03461 (1,2-HQD)

215H

active site
72D, 100P, 101F, 155Y, 189Y, 210R, 213H, 215H
CDD
cd03461 (1,2-HQD)

Protein sequence

>MIA_03024_1
MDQQFTEMVIAAMGPKTTPKMRRILGSLIQHIHDFSRENQITVEEWMAGVEFINRIGQMSDERRNEGILVSDVFGLESLV
DSITYHLEAHDHTSTAIIGPFYRPNSPKYAYGQSIIQKELGGQKTWVHGRVTDTAGNPLEGAELEVWHTAPNGLYEQQDP
EQPDYNLRGTFTADENGDYAYIALRPTNYPIPYDGPAGDLLQLMDRHPYRPSHIHWRVTKPGFRSLITQIYDSDCEYVKN
DSVFAVKPELVVHFKPASAEIKSKYGVEFDLEYNIALPTEAQAHAQVEKRLAEQKALEEELHAKAQLK

GO term prediction

Biological Process

GO:0006725 cellular aromatic compound metabolic process
GO:0009712 catechol-containing compound metabolic process
GO:0055114 oxidation-reduction process

Molecular Function

GO:0003824 catalytic activity
GO:0005506 iron ion binding
GO:0008199 ferric iron binding
GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
GO:0018576 catechol 1,2-dioxygenase activity

Cellular Component