MIA_02479_1

Protein

Length

2,266 amino acids

Protein function

EGGNOG:	0PJIX		guanyl-nucleotide exchange factor
SGD closest match:	S000006299	SYT1	Arf guanine nucleotide exchange factor SYT1
CGD closest match:	CAL0000201823	SEC7	Arf family guanine nucleotide exchange factor

Protein alignments

	%id	Aln length	E-value
MCA_03003_1	46.758%	1018	0.0	MCA_03003_1
A0A0J9X5U6_GEOCN	43.343%	706	9.00e-161	Similar to Saccharomyces cerevisiae YPR095C SYT1 Guanine nucleotide exchange factor (GEF) for Arf proteins OS=Geotrichum candidum GN=BN980_GECA03s02969g PE=4 SV=1
UniRef50_A0A0J9X5U6	43.343%	706	1.84e-157	Similar to Saccharomyces cerevisiae YPR095C SYT1 Guanine nucleotide exchange factor (GEF) for Arf proteins n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9X5U6_GEOCN
A0A060SX51_BLAAD	35.162%	711	1.00e-114	ARAD1A10670p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A10670g PE=4 SV=1
Q6CCN3_YARLI	37.060%	483	3.25e-84	YALI0C07975p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_C07975g PE=4 SV=1
A0A167DSG8_9ASCO	30.785%	484	2.03e-58	Uncharacterized protein OS=Sugiyamaella lignohabitans GN=AWJ20_1520 PE=4 SV=1
A0A1E4TK46_9ASCO	29.901%	505	6.48e-45	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_712 PE=4 SV=1
SYT1_YEAST	39.881%	168	4.38e-30	Arf guanine nucleotide exchange factor SYT1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SYT1 PE=1 SV=1
Q5ANF9_CANAL	35.345%	116	1.27e-13	Arf family guanine nucleotide exchange factor OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=SEC7 PE=4 SV=1
A0A1E3PDW1_9ASCO	36.264%	91	1.31e-13	Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_47984 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.8601
Predicted cleavage: 40

Protein family membership

None predicted.

Domains and repeats

Domain
3 - 1,123
Sec7 domain (IPR000904)
1,225 - 1,562
PH domain-like (IPR011993)
Pleckstrin homology domain (IPR001849)
Domain
1,010 - 1,126
Sec7 domain, alpha orthogonal bundle (IPR023394)

1 500 1000 1500 2000 2266

Detailed signature matches

IPR000904

Sec7 domain
1. SM00222 (sec7_5)
  
  3 - 1,118
  
  SMART
  SM00222 (sec7_5)
2. SSF48425 (Sec7 domain)
  
  986 - 1,123
  
  SUPERFAMILY
  SSF48425 (Sec7 domain)
3. PS50190 (SEC7)
  
  961 - 1,116
  
  PROSITE profiles
  PS50190 (SEC7)
4. PF01369 (Sec7)
  
  996 - 1,117
  
  Pfam
  PF01369 (Sec7)
IPR023394

Sec7 domain, alpha orthogonal bundle
1. G3DSA:1.10.10...
  
  1,010 - 1,126
  
  GENE3D
  G3DSA:1.10.1000.11 (G3DSA:1.10.1000.11)
IPR011993

PH domain-like
1. G3DSA:2.30.29.30
  
  1,225 - 1,562
  
  GENE3D
  G3DSA:2.30.29.30 (G3DSA:2.30.29.30)
2. SSF50729 (PH domain...)
  
  1,234 - 1,269
  
  SUPERFAMILY
  SSF50729 (PH domain-like)
  
  1,351 - 1,412
  
  SUPERFAMILY
  SSF50729 (PH domain-like)
IPR001849

Pleckstrin homology domain
1. SM00233 (PH_update)
  
  1,236 - 1,415
  
  SMART
  SM00233 (PH_update)
2. PS50003 (PH_DOMAIN)
  
  1,235 - 1,413
  
  PROSITE profiles
  PS50003 (PH_DOMAIN)

no IPR

Unintegrated signatures

CYTOPLASMIC_D... (C...)

1,078 - 1,409

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)
Coil

1,509 - 1,557

COILS
Coil (Coil)

2,256 - 2,266

COILS
Coil (Coil)
NON_CYTOPLASM... (N...)

1,431 - 2,266

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)

27 - 1,060

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
SIGNAL_PEPTIDE (Sig...)

1 - 26

PHOBIUS
SIGNAL_PEPTIDE (Signal Peptide)
SIGNAL_PEPTID... (S...)

22 - 26

PHOBIUS
SIGNAL_PEPTIDE_C_REGION (Signal peptide C-region)
SIGNAL_PEPTID... (S...)

14 - 21

PHOBIUS
SIGNAL_PEPTIDE_H_REGION (Signal peptide H-region)
SIGNAL_PEPTID... (S...)

1 - 13

PHOBIUS
SIGNAL_PEPTIDE_N_REGION (Signal peptide N-region)
TRANSMEMBRANE (Tran...)

1,061 - 1,077

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

1,410 - 1,430

PHOBIUS
TRANSMEMBRANE (Transmembrane region)
cd00171 (Sec7)

994 - 1,118

CDD
cd00171 (Sec7)
mobidb-lite (disord...)

1,624 - 1,651

MobiDB Lite
mobidb-lite (disorder_prediction)

799 - 969

MobiDB Lite
mobidb-lite (disorder_prediction)

1,876 - 1,895

MobiDB Lite
mobidb-lite (disorder_prediction)

444 - 473

MobiDB Lite
mobidb-lite (disorder_prediction)

671 - 737

MobiDB Lite
mobidb-lite (disorder_prediction)

1 - 410

MobiDB Lite
mobidb-lite (disorder_prediction)

751 - 787

MobiDB Lite
mobidb-lite (disorder_prediction)

1,838 - 1,867

MobiDB Lite
mobidb-lite (disorder_prediction)

2,246 - 2,266

MobiDB Lite
mobidb-lite (disorder_prediction)

1,284 - 1,328

MobiDB Lite
mobidb-lite (disorder_prediction)

2,071 - 2,196

MobiDB Lite
mobidb-lite (disorder_prediction)

526 - 545

MobiDB Lite
mobidb-lite (disorder_prediction)

558 - 656

MobiDB Lite
mobidb-lite (disorder_prediction)

1,437 - 1,477

MobiDB Lite
mobidb-lite (disorder_prediction)

1,916 - 1,976

MobiDB Lite
mobidb-lite (disorder_prediction)

495 - 514

MobiDB Lite
mobidb-lite (disorder_prediction)

1,678 - 1,811

MobiDB Lite
mobidb-lite (disorder_prediction)

1,990 - 2,046

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

active site/putati...

1,027V

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,028K

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,029L

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,030P

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,031R

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,032E

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,033A

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,034Q

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,063F

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,064F

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,065V

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,066F

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,067S

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,068L

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,069V

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,070L

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,071L

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,072Q

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,073T

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,074D

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,075Y

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

1,076F

active site/putative ARF binding site
1,027V, 1,028K, 1,029L, 1,030P, 1,031R, 1,032E, 1,033A, 1,034Q, 1,063F, 1,064F, 1,065V, 1,066F, 1,067S, 1,068L, 1,069V, 1,070L, 1,071L, 1,072Q, 1,073T, 1,074D, 1,075Y, 1,076F
CDD
cd00171 (Sec7)

Protein sequence

>MIA_02479_1
MPLLLPRRKKSHQAPSTALAASAATSSISPPIKSRFKRFPPSSAAAKHPAPPSSSSPITTTTSSTSSLQDSSVPSQRPSF
SASALDPPPDTPVCAFQTPPLLASQPPATSSPSSSPFPLPPDSSNSPSNHFLATGSATAHNILRTASSSSSLSPTSSAAS
SSPSSIMDPQEQDPQHQPHLQLHPPPATIDPIKLKTAKKKIKPRFFSGSRKLSASAHEHAAPSSLPQPAINLIPPTQNTP
SSSSTSSESPPTSQKQQPANNPSPGISAFASISESIVNTFTFGSSHDSPANPLDQPDDAHITNPSSVRPSTDSKLDLSID
AQAASSTHPSSTTPTSRFPSVFNRRSSTAQTALPPSKTSKWKFKPKRRSSVMDLQSAAAASSSTPSATATSTTIPENTIS
NQQTNATPLPLHNNASSISLNTHMRSYPTPLSQPFTHQNRLSVEELHSPSTSPHPRRSSVHLLSTSKPASMRSMNADRAS
IISTDNEAEDLNAASQNAASLQPQPPPSSTASSKLFDNIFRGDFKNPFHRSHSAQSATNQPSSTLTSSFKFKAPFMDDLH
HSPAASSPKKPIDGSSPLPPPPQFPISSSSSFTAPAAATPASIPEITLSISSPEDQHSTTQPPSIDTQTLPPLHQTMDPP
SPQQNPPPQANIAKSSHSLFALAANFISDHTSSHSQDALALPATSPIPSNSTANPPPDNDRSIPKESENPVLPFPAAPPS
SSSSTQNSIASTLKAPLSRIRRKTVSMLFSSESSFPLHNTPELPRELPPITPITPSIDIGLTTPQSNADSDLQHVQQLHA
SSLSQQPSPIKSDRNTDGTPSTPSLQNVKGVLSLHRTQSSQSPTRHPSQRSENSPTSSVFNLSSRPRSRTLSSLEQRRHS
THSGFFSSKYSESNLSSMATSSNNHNDPTSTNRSSPSPTSSPALPAVPTIPDALGGNSPRHSQSVVGPPSVPNNDPFSVP
LPPTEPNDTPATYLSKIRAIGFGSNITGLLACVDDDFHRQVLKLFLNGFHFENDPLDMALRKFLISVKLPREAQQIDRVL
EVFAFRYHECNPEIYDRPEDVYFFVFSLVLLQTDYFNPHNKSKMTKPDYLKTKNEAGYQNLPEEILGYFYDNITYTPFIH
TDEDILDPGQVPRVTWARRTTAFGRGSREPLDPYSLLLEGRLDSIRPRISLNPDEDPYDFIPVEDKAEMLKLRQEFETGP
SLQFISQRSRPDAFRSVNSGADDPSVSGPGVVGIKIVKAGYLYKCDAKRKNWRKWGVMLTASKLYIFKDVNWFQSNIMKD
MESAADSISGNVPTESKFLRRPRRTQTEPLDQTDSSSQAPSSAGSVASDRASTSILPPIEFHPAPVIRPLVDGFSPTSVI
VTSDMAALFYSNEEVKRNYSFLLIGRGGSREWYAGVDEADAHDWMKKINFAASFSTFFVAGMLGLGLIPVPKMPTNRMHS
LRRNSESSVSTGTSNNPIVPGQTPLGPNAPGDAENATSLDASLANGSHEAGSNDHDQEGSLEKWGKKNEEEANAALAAIE
ANKDRHLERKSAVERRLEDITRQLNNKEKQVQEQVRTARHIQILAPILAKTRDSILQYAGSLSAKLDWNLMERKKLLCYQ
NFFTIDLEVETDLCRQLGIPLTPTASDVSGQSPTKSQHASSSSSPPFPTDLVASTDLAATNVMSPATLSPVGSMSYASNV
GRSNSSPASSRMPSPQRRNVSLPVASQRASSQEVREDKKLEEEGFTGEANETTSNSEGMTSEESASFQSSVTDVSEEPKR
AGNTTPIVRPEKGATCGPEEKLETSLEATPNSNVPESPTRSIDELSTNTLKEGPRVLSSELTSANTIVAGLPQVVEVPSN
TTPHTCEPSEHTQIVEEEEEEPKPLPEAASGLLVTTTTSTASATEGPVSRYTTAEQPSIAALDTASLHTTSTVASSVSTA
VNPRPSAGAVPATPAHGHNRRTSSLYSVGSVISASSKKTPNKTRSSSSSSSSLDNLHGKSIRDHFWSFASSSSPADAMPS
GSEKPHSPGSPTKRSIRRSFSLSLRGSSRRNSINMSVGTSASSSPSNAAAAASAASGSPTASSKRMLKTLRGDLSGGSST
SSPNSVEGLGKTMSTRSLRKHSLDKSSSSPVVTNVGAASSPPNTAASQSASALAAVLSSGRDGQAGETRGSFDYGPNGDR
DPRRRHDRGHGAHTGDESGADGTHNGGSGPGRSHSLVRKQGENITLLGKQFHVVEVNPEFSGPFNQHRRAVSQNFSAASL
AFSGTCASPVTSNGTRVEAEKKEKSL

GO term prediction

Biological Process

GO:0032012 regulation of ARF protein signal transduction

Molecular Function

GO:0005086 ARF guanyl-nucleotide exchange factor activity

Cellular Component