Protein

MIA_02272_1

Length
1,116 amino acids

Browser: contig03:329377-332793+

Protein function

EGGNOG:0QEBKPYC1Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second (By similarity)
SGD closest match:S000000422PYC2Pyruvate carboxylase 2
CGD closest match:CAL0000188525PYC2Pyruvate carboxylase

Protein alignments

%idAln lengthE-value
A0A0J9X9G6_GEOCN86.355%11140.0Pyruvate carboxylase OS=Geotrichum candidum GN=BN980_GECA06s04762g PE=4 SV=1
Q6CAV2_YARLI81.418%11140.0Pyruvate carboxylase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_C24101g PE=4 SV=1
A0A060TC34_BLAAD80.341%11140.0Pyruvate carboxylase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D32912g PE=4 SV=1
A0A1E3PFE4_9ASCO78.886%11130.0Pyruvate carboxylase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_83995 PE=4 SV=1
A0A1E4TIT0_9ASCO77.957%11160.0Pyruvate carboxylase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_30005 PE=4 SV=1
A0A167FK68_9ASCO81.378%10740.0Pyruvate carboxylase 2 OS=Sugiyamaella lignohabitans GN=PYC2 PE=4 SV=1
A0A1D8PLY4_CANAL76.750%11140.0Pyruvate carboxylase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PYC2 PE=4 SV=1
PYC2_YEAST76.079%11120.0Pyruvate carboxylase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PYC2 PE=1 SV=2
UniRef50_C5PE0473.184%11150.0Pyruvate carboxylase n=40 Tax=Fungi TaxID=4751 RepID=C5PE04_COCP7

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0232

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
  3. Domain
1 200 400 600 800 1000 1116

Detailed signature matches

  1. Family IPR005930
    Pyruvate carboxylase
    1. TIGR01235
    2. PIRSF001594 (PCB)
  2. Domain IPR016185
    Pre-ATP-grasp domain
    1. SSF52440 (PreATP-gr...)
  3. Domain IPR011764
    Biotin carboxylation domain
    1. PS50979 (BC)
  4. Domain IPR005481
    Biotin carboxylase-like, N-terminal domain
    1. PF00289 (Biotin_carb_N)
  5. Domain IPR011761
    ATP-grasp fold
    1. PS50975 (ATP_GRASP)
  6. Domain IPR005479
    Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain
    1. PS00867 (CPSASE_2)
    2. PF02786 (CPSase_L_D2)
    3. PS00866 (CPSASE_1)
  7. Domain IPR013815
    ATP-grasp fold, subdomain 1
    1. G3DSA:3.30.14...
  8. Domain IPR011054
    Rudiment single hybrid motif
    1. SSF51246 (Rudiment ...)
  9. Domain IPR005482
    Biotin carboxylase, C-terminal
    1. SM00878 (Biotin_car...)
    2. PF02785 (Biotin_carb_C)
  10. Domain IPR013785
    Aldolase-type TIM barrel
    1. G3DSA:3.20.20.70
  11. Domain IPR000891
    Pyruvate carboxyltransferase
    1. PF00682 (HMGL-like)
    2. PS50991 (PYR_CT)
  12. Domain IPR003379
    Carboxylase, conserved domain
    1. PF02436 (PYC_OADA)
  13. Domain IPR011053
    Single hybrid motif
    1. SSF51230 (Single hy...)
  14. Domain IPR000089
    Biotin/lipoyl attachment
    1. PS50968 (BIOTINYL_L...)
    2. PF00364 (Biotin_lipoyl)
  15. SITE IPR001882
    Biotin-binding site
    1. PS00188 (BIOTIN)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:1.10.10.60
  2. G3DSA:2.40.50...
  3. G3DSA:3.10.60...
  4. G3DSA:3.30.47...
  5. G3DSA:3.40.50.20
  6. SSF51569 (Aldolase)
  7. SSF56059 (Glutathio...)
  8. SSF89000 (post-HMGL...)
  9. cd06850 (biotinyl_d...)
  10. cd07937 (DRE_TIM_PC...)

Residue annotation

  1. catalytic residues...
  2. active site cd07937
  3. metal binding site...
  4. homodimer binding ...
  5. carboxyltransferas...
  6. biotinylation site...

Protein sequence

>MIA_02272_1
MHRLKADEAYAIGPRGKYTPVQAYLQIDEIISIAKKHGVNMIHPGYGFLSENSEFARKVEESGMIWIGPPHNVIDSVGDK
VSARNLALKCNVPVVPGTDGPIEDIKDAQDFVDKYGYPVIIKAAFGGGGRGMRVVREGDDIGDAFLRATSEAKTAFGNGT
VFIERFLDKPKHIEVQLLADNHGNVIHLYERDCSVQRRHQKVVEVAPALTLPEDVRNAILTDAVKLAKTANYRNAGTAEF
LVDNQNRHYFIEINPRIQVEHTITEEITGIDIVAAQIQIAAGATLEKLGLTQNKISYRGFAIQCRITTEDPAKNFQPDTG
KIEVYRSSGGNGVRLDGGNGFAGAIISPHYDSMLVKCTCSGSTYEIARRKMLRALIEFRIRGVKTNIPFLLALLTNETFI
KGDCWTTFIDDTPKLFEMLTSQNRAQKLLAFLAELTVNGSQVAGQIGVPKLVSEPTVPIIYESPDGPAIDVENVLPPKGW
RDVLLEKGPEGFAKAIRDFKGTLIMDTTWRDAHQSLLATRVRTYDLLKIAPTTSHALAGAFALECWGGATFDVAMRFLHE
DPWARLRKLRAIVPNIPFQMLLRGANGVAYSSLPDNAIDQFVFEAKKNGVDIFRVFDALNDLEQLKVGVDAVKKAGGVVE
ATVCYSGDMLNPKKKYNLAYYLDFVDKIVAMGTHILGIKDMAGTLKPAAATKLVGSIRAKYPDLPIHVHTHDSAGTGVAS
MTAAALAGADAVDAASNSMSGMTSQPSISAILASLEGRVETNLDANKVKDIDAYWGQMRLLYSCFEADLKGPDPEVYVHE
IPGGQLTNLIFQAQQVGLGEQWKETKKAYEEANRLLGDIVKVTPTSKVVGDFAQFMVSNKLSYDDVLAKASTLDVPASVL
DFFEGLMGQPYGGFPEPLRTEILRGQRPKLTERPGKTLKPLNLAGIRADLEKRFGGSINGTDVASYTMYPKVYEDYRKYT
EKYGDLSVVPTQYFLAPPEIGKEFSVDIEKGKTLILKLLAIGELSTQTGTREVYFELNGEMRKVTVDDKFAAVETVTRPK
ADAHNPNEVGAPMAGVVVEVRVKEGAEVKKGDPVVVLSAMKMEMVVSANSSGKVAEVLVKDGDSVDGSDLLVKIKA

GO term prediction

Biological Process

GO:0006090 pyruvate metabolic process
GO:0006094 gluconeogenesis

Molecular Function

GO:0003824 catalytic activity
GO:0004075 biotin carboxylase activity
GO:0004736 pyruvate carboxylase activity
GO:0005524 ATP binding
GO:0009374 biotin binding
GO:0046872 metal ion binding

Cellular Component

None predicted.