MIA_01489_1

Protein

Length

286 amino acids

Protein function

EGGNOG:	0PJ85	FG05551.1	peroxisomal D3,D2-enoyl-CoA isomerase
SGD closest match:	S000004274	ECI1	3,2-trans-enoyl-CoA isomerase
CGD closest match:	CAL0000181317	ECI1	Dodecenoyl-CoA isomerase

Protein alignments

	%id	Aln length	E-value
MCA_02486_1	80.070%	286	3.58e-154	MCA_02486_1
A0A0J9XB23_GEOCN	52.962%	287	1.96e-96	Similar to Saccharomyces cerevisiae YLR284C ECI1 Peroxisomal delta3,delta2-enoyl-CoA isomerase OS=Geotrichum candidum GN=BN980_GECA07s03772g PE=4 SV=1
A0A167EN94_9ASCO	52.817%	284	3.05e-96	Dodecenoyl-CoA isomerase OS=Sugiyamaella lignohabitans GN=ECI1 PE=4 SV=1
A0A060T2D8_BLAAD	49.474%	285	1.30e-84	ARAD1C24530p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C24530g PE=4 SV=1
UniRef50_A0A060T2D8	49.474%	285	3.20e-81	ARAD1C24530p n=3 Tax=Saccharomycetales TaxID=4892 RepID=A0A060T2D8_BLAAD
Q5A318_CANAL	39.161%	286	9.44e-64	Dodecenoyl-CoA isomerase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=ECI1 PE=4 SV=1
A0A1E4TFH8_9ASCO	36.396%	283	1.03e-49	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_106749 PE=4 SV=1
ECI1_YEAST	32.632%	285	6.19e-46	3,2-trans-enoyl-CoA isomerase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ECI1 PE=1 SV=1
A0A1E3PDQ1_9ASCO	29.787%	94	9.37e-08	ClpP/crotonase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_84168 PE=3 SV=1
Q6CF43_YARLI	25.000%	212	2.12e-07	YALI0B10406p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_B10406g PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0212

Protein family membership

Crotonase superfamily (IPR001753)

Domains and repeats

Domain
4 - 276
ClpP/crotonase-like domain (IPR029045)

1 50 100 150 200 250 286

Detailed signature matches

IPR001753

Crotonase superfamily
1. PF00378 (ECH_1)
  
  111 - 269
  
  Pfam
  PF00378 (ECH_1)
IPR029045

ClpP/crotonase-like domain
1. SSF52096 (ClpP/crot...)
  
  4 - 276
  
  SUPERFAMILY
  SSF52096 (ClpP/crotonase)

no IPR

Unintegrated signatures

G3DSA:3.90.22...

1 - 285

GENE3D
G3DSA:3.90.226.10 (G3DSA:3.90.226.10)
cd06558 (crotonase-...)

4 - 225

CDD
cd06558 (crotonase-like)

Residue annotation

substrate binding ...

23Y

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)

25A

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)

57K

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)

61A

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)

62G

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)

63A

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)

64N

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)

65L

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)

129P

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)

131I

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)

132G

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)

133L

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)

155P

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)

156F

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)

159I

substrate binding site
23Y, 25A, 57K, 61A, 62G, 63A, 64N, 65L, 129P, 131I, 132G, 133L, 155P, 156F, 159I
CDD
cd06558 (crotonase-like)
oxyanion hole (OAH...

63A

oxyanion hole (OAH) forming residues
63A, 133L
CDD
cd06558 (crotonase-like)

133L

oxyanion hole (OAH) forming residues
63A, 133L
CDD
cd06558 (crotonase-like)
trimer interface c...

113E

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

121V

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

142D

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

143F

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

144I

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

145Y

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

157S

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

158N

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

159I

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

160G

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

166A

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

168S

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

169F

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

170S

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

172A

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

173Y

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

178S

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

179K

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

181S

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

182E

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

184L

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

185L

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

188S

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

199G

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

202N

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

222L

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

225E

trimer interface
113E, 121V, 142D, 143F, 144I, 145Y, 157S, 158N, 159I, 160G, 166A, 168S, 169F, 170S, 172A, 173Y, 178S, 179K, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 222L, 225E
CDD
cd06558 (crotonase-like)

Protein sequence

>MIA_01489_1
MVEITYEVKGKTAIITLNIPQLYNALSGAEYRRLEQLVLLAAQNPDTIVTLIQGTGKFFSAGANLGSGSGSKKEDTIELK
QIDDPSDPVEYKIQKVRTAYAYSFGTRNLTITETFYNHPKVLVIALNGPVIGLTSSLVAMADFIYARDTAWLLTPFSNIG
LAPEGAASFSLAYRLGHSKASELLLSSSPIDASELYRLGFVNKLYSSKEYTTESFNEAIQKLIQEKFYHLHPESLIITKE
LMRKSTARNYAEANALEVVAGTDQFTKGVPQKRFAALAKRELKHKL

GO term prediction

Biological Process

GO:0008152 metabolic process

Molecular Function

GO:0003824 catalytic activity

Cellular Component

None predicted.

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Entry type

Status

Per-residue features