MIA_00939_1

Protein

Length

423 amino acids

Protein function

EGGNOG:	0PGSK	FG09661.1	acyl-CoA dehydrogenase
SGD closest match:	S000003173	POX1	Acyl-coenzyme A oxidase
CGD closest match:	CAL0000175310	CAALFM_CR08920WA	Uncharacterized protein

Protein alignments

	%id	Aln length	E-value
MCA_00595_1	89.974%	389	0.0	MCA_00595_1
A0A0J9X374_GEOCN	80.890%	382	0.0	Uncharacterized protein OS=Geotrichum candidum GN=BN980_GECA01s07996g PE=3 SV=1
A0A060TAB8_BLAAD	77.720%	386	0.0	ARAD1D17446p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D17446g PE=3 SV=1
A0A167CWX1_9ASCO	75.853%	381	0.0	Acyl-CoA dehydrogenase, mitochondrial OS=Sugiyamaella lignohabitans GN=AWJ20_448 PE=3 SV=1
Q6C8Y9_YARLI	74.742%	388	0.0	YALI0D15708p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_D15708g PE=3 SV=1
A0A1E3PQ09_9ASCO	71.958%	378	0.0	Acyl-CoA dehydrogenase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_45192 PE=3 SV=1
UniRef50_A0A084G281	67.526%	388	0.0	Short-chain acyl-CoA dehydrogenase n=386 Tax=Eukaryota TaxID=2759 RepID=A0A084G281_9PEZI
A0A1E4TLQ9_9ASCO	72.404%	366	0.0	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_1295 PE=3 SV=1
A0A1D8PTU4_CANAL	25.510%	294	6.88e-17	Uncharacterized protein OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=CAALFM_CR08920WA PE=3 SV=1
ACOX_YEAST	26.590%	173	1.74e-06	Acyl-coenzyme A oxidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=POX1 PE=3 SV=2

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9989
Predicted cleavage: 32

Protein family membership

None predicted.

Domains and repeats

Domain
12 - 154
Acyl-CoA dehydrogenase/oxidase, N-terminal (IPR013786)
159 - 255
Acyl-CoA oxidase/dehydrogenase, central domain (IPR006091)
265 - 419
Acyl-CoA dehydrogenase/oxidase C-terminal (IPR009075)
Domain
41 - 280
Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain (IPR009100)

1 50 100 150 200 250 300 350 423

Detailed signature matches

IPR013786

Acyl-CoA dehydrogenase/oxidase, N-terminal
1. G3DSA:1.10.54...
  
  12 - 154
  
  GENE3D
  G3DSA:1.10.540.10 (G3DSA:1.10.540.10)
2. PF02771 (Acyl-CoA_dh_N)
  
  44 - 152
  
  Pfam
  PF02771 (Acyl-CoA_dh_N)
IPR009100

Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain
1. SSF56645 (Acyl-CoA ...)
  
  41 - 280
  
  SUPERFAMILY
  SSF56645 (Acyl-CoA dehydrogenase NM domain-like)
IPR006091

Acyl-CoA oxidase/dehydrogenase, central domain
1. PF02770 (Acyl-CoA_dh_M)
  
  159 - 255
  
  Pfam
  PF02770 (Acyl-CoA_dh_M)
IPR009075

Acyl-CoA dehydrogenase/oxidase C-terminal
1. PF00441 (Acyl-CoA_dh_1)
  
  267 - 415
  
  Pfam
  PF00441 (Acyl-CoA_dh_1)
2. SSF47203 (Acyl-CoA ...)
  
  265 - 419
  
  SUPERFAMILY
  SSF47203 (Acyl-CoA dehydrogenase C-terminal domain-like)
IPR006089

Acyl-CoA dehydrogenase, conserved site
1. PS00073 (ACYL_COA_DH_2)
  
  375 - 394
  
  PROSITE patterns
  PS00073 (ACYL_COA_DH_2)
2. PS00072 (ACYL_COA_DH_1)
  
  161 - 173
  
  PROSITE patterns
  PS00072 (ACYL_COA_DH_1)

no IPR

Unintegrated signatures

G3DSA:1.20.14...

255 - 423

GENE3D
G3DSA:1.20.140.10 (G3DSA:1.20.140.10)
G3DSA:2.40.11...

158 - 254

GENE3D
G3DSA:2.40.110.10 (G3DSA:2.40.110.10)
NON_CYTOPLASM... (N...)

19 - 423

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
PIRSF016578 (PIGM)

167 - 422

PIRSF
PIRSF016578 (PIGM)

36 - 134

PIRSF
PIRSF016578 (PIGM)
SIGNAL_PEPTIDE (Sig...)

1 - 18

PHOBIUS
SIGNAL_PEPTIDE (Signal Peptide)
SIGNAL_PEPTID... (S...)

11 - 18

PHOBIUS
SIGNAL_PEPTIDE_C_REGION (Signal peptide C-region)
SIGNAL_PEPTID... (S...)

3 - 10

PHOBIUS
SIGNAL_PEPTIDE_H_REGION (Signal peptide H-region)
SIGNAL_PEPTID... (S...)

1 - 2

PHOBIUS
SIGNAL_PEPTIDE_N_REGION (Signal peptide N-region)
cd01158 (SCAD_SBCAD)

45 - 418

CDD
cd01158 (SCAD_SBCAD)

Residue annotation

FAD binding site c...

160F

FAD binding site
160F, 163S, 169S, 194W, 196T, 375E, 379G, 402E
CDD
cd01158 (SCAD_SBCAD)

163S

FAD binding site
160F, 163S, 169S, 194W, 196T, 375E, 379G, 402E
CDD
cd01158 (SCAD_SBCAD)

169S

FAD binding site
160F, 163S, 169S, 194W, 196T, 375E, 379G, 402E
CDD
cd01158 (SCAD_SBCAD)

194W

FAD binding site
160F, 163S, 169S, 194W, 196T, 375E, 379G, 402E
CDD
cd01158 (SCAD_SBCAD)

196T

FAD binding site
160F, 163S, 169S, 194W, 196T, 375E, 379G, 402E
CDD
cd01158 (SCAD_SBCAD)

375E

FAD binding site
160F, 163S, 169S, 194W, 196T, 375E, 379G, 402E
CDD
cd01158 (SCAD_SBCAD)

379G

FAD binding site
160F, 163S, 169S, 194W, 196T, 375E, 379G, 402E
CDD
cd01158 (SCAD_SBCAD)

402E

FAD binding site
160F, 163S, 169S, 194W, 196T, 375E, 379G, 402E
CDD
cd01158 (SCAD_SBCAD)
homotetramer inter...

165P

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

170D

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

194W

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

237E

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

238K

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

239K

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

240L

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

241G

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

307R

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

310F

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

319G

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

323Q

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

324M

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

326Q

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

329V

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

337L

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

360L

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

371S

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

375E

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

380V

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

382F

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

384R

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

385E

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

389E

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

392Y

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

393R

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

396K

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

397I

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

399A

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

406N

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

409L

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)

412I

homotetramer interface
165P, 170D, 194W, 237E, 238K, 239K, 240L, 241G, 307R, 310F, 319G, 323Q, 324M, 326Q, 329V, 337L, 360L, 371S, 375E, 380V, 382F, 384R, 385E, 389E, 392Y, 393R, 396K, 397I, 399A, 406N, 409L, 412I
CDD
cd01158 (SCAD_SBCAD)
substrate binding ...

169S

substrate binding pocket
169S, 170D, 274I, 277L, 278N, 281R, 310F, 402E
CDD
cd01158 (SCAD_SBCAD)

170D

substrate binding pocket
169S, 170D, 274I, 277L, 278N, 281R, 310F, 402E
CDD
cd01158 (SCAD_SBCAD)

274I

substrate binding pocket
169S, 170D, 274I, 277L, 278N, 281R, 310F, 402E
CDD
cd01158 (SCAD_SBCAD)

277L

substrate binding pocket
169S, 170D, 274I, 277L, 278N, 281R, 310F, 402E
CDD
cd01158 (SCAD_SBCAD)

278N

substrate binding pocket
169S, 170D, 274I, 277L, 278N, 281R, 310F, 402E
CDD
cd01158 (SCAD_SBCAD)

281R

substrate binding pocket
169S, 170D, 274I, 277L, 278N, 281R, 310F, 402E
CDD
cd01158 (SCAD_SBCAD)

310F

substrate binding pocket
169S, 170D, 274I, 277L, 278N, 281R, 310F, 402E
CDD
cd01158 (SCAD_SBCAD)

402E

substrate binding pocket
169S, 170D, 274I, 277L, 278N, 281R, 310F, 402E
CDD
cd01158 (SCAD_SBCAD)
catalytic base cd0...

402E

catalytic base
402E
CDD
cd01158 (SCAD_SBCAD)

Protein sequence

>MIA_00939_1
MSSVLARSALRAAPRASASRASTRSFHAARAVANASQTSIFQFSEDELMFKDTVAKFAAEVVGPRVREMDENEDMDPAVI
QGMFDNGLMGVETPEEYGGAGASFTSAILVVEELARVDPSVSVCNDVHNTLVSTCVRTWGSKELKDKYMPILNSERVGSF
CLSEPVSGSDAFALKTRATKNADGDYVINGSKMWITNAKEAGIYLVFANVAPEQGYKGITCFVVEKEFPGFSIAKKEKKL
GIRASSTCVLNFDDVVVPKENILGELGKGYKIAIECLNEGRIGIAAQMTGLAMGAFERATSYAFNDRKQFGQYIGDFQGM
QFQMAQAAVEIEAARLLLYNASRMKENGLPFVKEAAMAKLKASQVAELVASRAVEWMGGVGFTREETLEKFYRDAKIGAI
YEGTSNIQLQTIAKFLQKEAQGK

GO term prediction

Biological Process

GO:0008152 metabolic process
GO:0055114 oxidation-reduction process

Molecular Function

GO:0003995 acyl-CoA dehydrogenase activity
GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors
GO:0050660 flavin adenine dinucleotide binding

Cellular Component