Protein
MIA_00547_1
Length
332 amino acids
Browser: contig01:1579325-1580363+
Protein function
| EGGNOG: | 0PF94 | THI4 | Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance (By similarity) |
|---|---|---|---|
| SGD closest match: | S000003376 | THI4 | Thiamine thiazole synthase |
| CGD closest match: | CAL0000199344 | THI4 | Thiamine thiazole synthase |
Protein alignments
| %id | Aln length | E-value | ||
|---|---|---|---|---|
| A0A0J9X667_GEOCN | 75.079% | 317 | 1.76e-161 | Thiamine thiazole synthase OS=Geotrichum candidum GN=THI4 PE=3 SV=1 |
| A0A167C9Z5_9ASCO | 71.338% | 314 | 3.32e-152 | Thiamine thiazole synthase OS=Sugiyamaella lignohabitans GN=THI4 PE=3 SV=1 |
| THI4_YARLI | 78.292% | 281 | 4.74e-151 | Thiamine thiazole synthase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=THI4 PE=3 SV=1 |
| A0A1E3PE62_9ASCO | 76.325% | 283 | 5.69e-151 | Thiamine thiazole synthase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=THI4 PE=3 SV=1 |
| UniRef50_A0A1E4SC62 | 69.536% | 302 | 8.87e-136 | Thiamine thiazole synthase n=1 Tax=Candida tanzawaensis NRRL Y-17324 TaxID=984487 RepID=A0A1E4SC62_9ASCO |
| A0A060TCZ6_BLAAD | 73.477% | 279 | 1.04e-139 | Thiamine thiazole synthase OS=Blastobotrys adeninivorans GN=THI4 PE=3 SV=1 |
| THI4_CANAL | 67.097% | 310 | 1.85e-137 | Thiamine thiazole synthase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=THI4 PE=3 SV=1 |
| THI4_YEAST | 64.746% | 295 | 1.71e-114 | Thiamine thiazole synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=THI4 PE=1 SV=1 |
Mitochondrial localization by mitoprotII
Probability of mitochondrial location: 0.3773
Protein family membership
- Thiazole biosynthetic enzyme Thi4 family (IPR002922)
- Thiamine thiazole synthase (IPR027495)
Domains and repeats
-
Domain
1
50
100
150
200
250
300
332
Detailed signature matches
no IPR
Unintegrated signatures
-
PF01946 (Thi4)
Protein sequence
>MIA_00547_1 MHSTTIFLIRFMKKEMAPATASLTPYPITIFDKAAKSSNDVAAAPKPEKWDNFKFNPIRESQVSRAMTSRYFKDLDTYAE SDVVIIGAGSAGLSAAYVLAKSRPDLKIAIIESNVSPGGGCWVSGALMSAQVIRKPAEKFLDEVGIPYEDEGDYVVVKHA ALFISTLLSKVLLFPNVKLFNATAVEDLITRKDPVTGEVRVAGVVTNWTLVTMHHDDQSCMDPNTINAHIILSATGHDGP FGAFTVKRLASMGLIKLGGMQGLDMNSAEDAIVKKSLEVYPGVVVAGMETSEVYGCNRMGPTFGAMLLSGVRAAESVLEV YETRKKQNEASY
GO term prediction
Biological Process
GO:0006950 response to stress
GO:0009228 thiamine biosynthetic process
GO:0055114 oxidation-reduction process
Molecular Function
GO:0016491 oxidoreductase activity
Cellular Component
None predicted.