Protein

MCA_06016_1

Length
577 amino acids

Gene name: KRS1

Description: Cytoplasmic lysyl-tRNA synthetase; protein with OB-fold nucleic acid-binding domain

Browser: contigD:3037781-3039515-

RNA-seq: read pairs 19045, FPKM 407.1, percentile rank 93.6% (100% = highest expression)

Protein function

Annotation:KRS1Cytoplasmic lysyl-tRNA synthetase; protein with OB-fold nucleic acid-binding domain
KEGG:K04567KARS lysyl-tRNA synthetase, class II [EC:6.1.1.6]
EGGNOG:0PFUCKRS1lysyl-tRNA synthetase
SGD closest match:S000002444KRS1Lysine--tRNA ligase, cytoplasmic
CGD closest match:CAL0000185145KRS1Lysine--tRNA ligase

Protein alignments

%idAln lengthE-value
MIA_01252_187.00%5770.0MIA_01252_1
A0A0J9X9B4_GEOCN82.32%5770.0Lysine--tRNA ligase OS=Geotrichum candidum GN=BN980_GECA05s06918g PE=3 SV=1
A0A167C0H8_9ASCO79.38%5820.0Lysine--tRNA ligase OS=Sugiyamaella lignohabitans GN=KRS1 PE=3 SV=1
A0A1E3PL88_9ASCO76.82%5780.0Lysine--tRNA ligase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_45743 PE=3 SV=1
A0A060TDT8_BLAAD75.52%5720.0Lysine--tRNA ligase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D46728g PE=3 SV=1
Q6C1H2_YARLI73.83%5770.0Lysine--tRNA ligase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F16291g PE=3 SV=1
Q5ADU2_CANAL72.84%5780.0Lysine--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=KRS1 PE=3 SV=1
A0A1E4THC7_9ASCO71.06%5840.0Lysine--tRNA ligase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_116458 PE=3 SV=1
SYKC_YEAST72.00%5750.0Lysine--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=KRS1 PE=1 SV=2
UniRef50_A0A1E1KJD662.29%5860.0Lysine--tRNA ligase n=11 Tax=Eukaryota TaxID=2759 RepID=A0A1E1KJD6_9HELO

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1388

Protein family membership

Domains and repeats

  1. Domain
1 100 200 300 400 500 577

Detailed signature matches

  1. Family IPR002313
    Lysine-tRNA ligase, class II
    1. MF_00252 (Lys_tRNA_...)
    2. TIGR00499
  2. Family IPR034762
    Bacterial/eukaryotic lysine-tRNA ligase, class II
    1. PIRSF039101 (LysRS2)
  3. Domain IPR012340
    Nucleic acid-binding, OB-fold
    1. SSF50249 (Nucleic a...)
  4. Domain IPR004365
    OB-fold nucleic acid binding domain, AA-tRNA synthetase-type
    1. PF01336 (tRNA_anti-...)
  5. Domain IPR006195
    Aminoacyl-tRNA synthetase, class II
    1. PS50862 (AA_TRNA_LI...)
  6. Domain IPR004364
    Aminoacyl-tRNA synthetase, class II (D/K/N)
    1. PF00152 (tRNA-synt_2)
  7. Domain IPR018149
    Lysyl-tRNA synthetase, class II, C-terminal
    1. PR00982 (TRNASYNTHLYS)
    2. cd00775 (LysRS_core)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:2.40.50...
  3. G3DSA:3.30.93...
  4. SSF55681 (Class II ...)
  5. cd04322 (LysRS_N)
  6. mobidb-lite (disord...)

Residue annotation

  1. dimer interface cd...
  2. putative anticodon...
  3. motif 1 cd00775
  4. active site cd00775
  5. motif 2 cd00775
  6. motif 3 cd00775

Protein sequence

>MCA_06016_1
MSDKAVEQATEQLQKTYLDEETGEQVSKSELKKRIKAREVAKKKAEKAAAKPAQPTASKKAKEEELTPNQYFELRSRQIT
ELKKSKAPYPYPHKFHVSTTIPEFVEKFNHLKRGDKLEDEKVSIAGRIFTKRESGAQLKFYVIHGGGSSVQAVAQAFDDE
QLDAIHEILKRGDIVGINGYPFRTSPKKGGEGELSIFIESVTLLTPSLHMLPTEHFGFKDLESRFRMRYLDLIMNKSTRD
RFIVRSKIISYIRKFFDSRGFIEVETPMMNVIAGGATAKPFVTHHNDLNMDLFMRVAPELYLKELVVGGLERVYEIGRQF
RNEGIDMTHNPEFTTCEFYQAYADVYDLMDTTELLFSEMVKEITGSYKITYHPEGPEGKTMEIDFSRPWKRLDMIPTLEE
RLNVKFPPANELHTAETGEFLKGVLKKAGLECPPPLTNARMLDKLVGDYIEDTCINPTFVFGHPEMMSPLAKYHRDIPGL
TERFEVFVGTKEICNAYTELNDPFDQRERFEEQARQKEQGDDEAQLVDEVFLNALEYGLPPTGGWGCGIDRIAMFLTDSN
TIKEVLLFPTMKPEANN

GO term prediction

Biological Process

GO:0006418 tRNA aminoacylation for protein translation
GO:0006430 lysyl-tRNA aminoacylation

Molecular Function

GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004824 lysine-tRNA ligase activity
GO:0005524 ATP binding

Cellular Component

GO:0005737 cytoplasm