Protein
MCA_05959_1
Length
942 amino acids
Gene name: MIS1B
Description: C-1-tetrahydrofolate synthase
Browser: contigD:2868700-2871596+
RNA-seq: read pairs 14607, FPKM 191.4, percentile rank 87.6% (100% = highest expression)
Protein function
| Annotation: | MIS1B | C-1-tetrahydrofolate synthase | |
|---|---|---|---|
| KEGG: | K00288 | MTHFD | methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase / formyltetrahydrofolate synthetase [EC:1.5.1.5 3.5.4.9 6.3.4.3] |
| EGGNOG: | 0PFGE | FG10267.1 | C-1-tetrahydrofolate synthase |
| SGD closest match: | S000000288 | MIS1 | C-1-tetrahydrofolate synthase, mitochondrial |
| CGD closest match: | CAL0000181460 | MIS11 | Trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase |
Protein alignments
| %id | Aln length | E-value | ||
|---|---|---|---|---|
| MIA_05794_1 | 86.20% | 942 | 0.0 | MIA_05794_1 |
| A0A0J9XBT0_GEOCN | 80.89% | 942 | 0.0 | Similar to Saccharomyces cerevisiae YBR084W MIS1 Mitochondrial C1-tetrahydrofolate synthase OS=Geotrichum candidum GN=BN980_GECA09s02265g PE=3 SV=1 |
| F2Z650_YARLI | 78.30% | 940 | 0.0 | YALI0E01056p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E01056g PE=3 SV=1 |
| A0A060T8K2_BLAAD | 75.56% | 941 | 0.0 | ARAD1C37708p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C37708g PE=3 SV=1 |
| A0A1E4TJX2_9ASCO | 74.28% | 941 | 0.0 | Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_603 PE=3 SV=1 |
| Q59SM8_CANAL | 73.63% | 948 | 0.0 | Trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=MIS11 PE=3 SV=1 |
| A0A1E3PM07_9ASCO | 77.64% | 890 | 0.0 | C1-THFS protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_45921 PE=3 SV=1 |
| A0A167DXR6_9ASCO | 71.44% | 942 | 0.0 | Trifunctional formate-tetrahydrofolate ligase OS=Sugiyamaella lignohabitans GN=MIS1 PE=3 SV=1 |
| UniRef50_A0A094E621 | 72.56% | 944 | 0.0 | Eukaryotic translation initiation factor 3 subunit I n=8 Tax=leotiomyceta TaxID=716546 RepID=A0A094E621_9PEZI |
| C1TM_YEAST | 65.50% | 945 | 0.0 | C-1-tetrahydrofolate synthase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MIS1 PE=1 SV=1 |
Mitochondrial localization by mitoprotII
Probability of mitochondrial location: 0.0396
Protein family membership
- Tetrahydrofolate dehydrogenase/cyclohydrolase (IPR000672)
- Formate-tetrahydrofolate ligase, FTHFS (IPR000559)
Domains and repeats
-
Domain
1
100
200
300
400
500
600
700
800
942
Detailed signature matches
no IPR
Unintegrated signatures
Residue annotation
-
homodimer interfac...
-
NADP binding site ...
-
substrate binding ...
-
Cesium cation bind...
-
Potassium binding ...
Protein sequence
>MCA_05959_1 MGAQLIDGTSIAKDIRSGLNAEIKAIQQEKPYFKPSLIIVQVGDRPDSNTYVRMKLKAADEANISCQLVKLDEKVTQAEL LEKITNFNEDPTVHGILVQLPLPSHISEVTITDAVSAKKDVDGFGTLNIGELAKRGGSPLFVPCTPKGIIVLLEKSSVQV SGKKVVVLGRSDIVGSPVSYLLKNLDATVVVLHSKTPQSVFEQELKSADIVVAAIGKPQYVKGEWLKEGAVVIDVGTNYI DDSTKKSGKRLVGDVDFESASQVASLLTPVPGGVGPMTVAMLLDNVVASAKRFYEEEETRKITPLPLDLKVPVPSDFEIS RAQKPKNIAKIAEEVGILPHELELYGAYKAKVQLDLLDRLKERKNGKYILVTGITPTPLGEGKSTTTVGLVQALGAHLGK VAFANVRQPSMGPTFGVKGGAAGGGYSQVIPMDEFNLHLTGDIHAISAATNLLAAAIDTRMFHENTQKDGPLYKRLVPAK KGVRKFSPCMFSRLKKLGIDKTNPDDLTPEEITKFARLDIDPETITWKRVVDCNDRFLRGITVGQAPTEKGFTRSTGFDI TVASECMAILALSNDLKDMRERLGKMVIASSKSGEPVTCDDIGCGGALTALMKDAIKPNMMQTLEGTPVFVHAGPFANIS IGANSVLADKMALKLAGTREGEPEEKAGFVVTEAGFDFTMGGERFFNIKCRSSGLIPDVVVIVATVRALKVHGGGPDVKP GVPLPDVYTKEDLDLLRKGCANLGKHISNALAYGRPVVVAINKFETDTEAEHQVIREEAIKYGAEDAIVANHWAEGGKGA VDLAKGVIKASQKKDTTFDFLYKTEGNNSVEDKIRTICQKMYGAADVEFSELAQKKIDTYTRQGFGELPICIAKTQYSLS HDPNLKGVPTGFTVPIRDVRASVGAGYLYALAAEIMTIPGLPTHCGFMDVEVNDDGEIEGMF
GO term prediction
Biological Process
GO:0055114 oxidation-reduction process
Molecular Function
GO:0003824 catalytic activity
GO:0004329 formate-tetrahydrofolate ligase activity
GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity
GO:0005524 ATP binding
Cellular Component
None predicted.