Protein

MCA_05697_1

Length
465 amino acids

Gene name: MSF1

Description: Phenylalanine--tRNA ligase, mitochondrial

Browser: contigD:2068178-2069576-

RNA-seq: read pairs 2961, FPKM 78.5, percentile rank 74.9% (100% = highest expression)

Protein function

Annotation:MSF1Phenylalanine--tRNA ligase, mitochondrial
KEGG:K01889FARSA phenylalanyl-tRNA synthetase alpha chain [EC:6.1.1.20]
EGGNOG:0PFEDFG01449.1Phenylalanyl-tRNA synthetase
SGD closest match:S000006251MSF1Phenylalanine--tRNA ligase, mitochondrial
CGD closest match:CAL0000183890MSF1Phenylalanine--tRNA ligase

Protein alignments

%idAln lengthE-value
MIA_00364_183.52%4550.0MIA_00364_1
A0A0J9X713_GEOCN75.83%4510.0Similar to Saccharomyces cerevisiae YPR047W MSF1 Mitochondrial phenylalanyl-tRNA synthetase OS=Geotrichum candidum GN=BN980_GECA04s01902g PE=4 SV=1
A0A1E3PDT8_9ASCO64.67%4670.0Phenylalanyl-tRNA synthetase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_53230 PE=4 SV=1
UniRef50_A0A1E3PDT864.67%4670.0Phenylalanyl-tRNA synthetase n=1 Tax=Nadsonia fulvescens var. elongata DSM 6958 TaxID=857566 RepID=A0A1E3PDT8_9ASCO
A0A167F188_9ASCO65.44%4630.0Phenylalanine--tRNA ligase OS=Sugiyamaella lignohabitans GN=MSF1 PE=4 SV=1
A0A060T1Z4_BLAAD64.62%4550.0ARAD1C25080p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C25080g PE=4 SV=1
Q6CBI1_YARLI60.75%4280.0YALI0C18535p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_C18535g PE=4 SV=1
Q5AD28_CANAL56.14%4560.0Phenylalanine--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=MSF1 PE=4 SV=1
SYFM_YEAST54.80%4583e-172Phenylalanine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MSF1 PE=1 SV=3
A0A1E4TEH6_9ASCO48.94%4258e-144Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_101103 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9687

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 50 100 150 200 250 300 350 400 465

Detailed signature matches

  1. Family IPR004530
    Phenylalanyl-tRNA synthetase, class IIc, mitochondrial
    1. TIGR00469
  2. Domain IPR002319
    Phenylalanyl-tRNA synthetase
    1. PF01409 (tRNA-synt_2d)
  3. Domain IPR006195
    Aminoacyl-tRNA synthetase, class II
    1. PS50862 (AA_TRNA_LI...)
  4. Domain IPR005121
    Ferrodoxin-fold anticodon-binding domain
    1. PS51447 (FDX_ACB)
    2. PF03147 (FDX-ACB)
    3. SSF54991 (Anticodon...)
    4. SM00896 (FDX_ACB_2)
    5. G3DSA:3.30.70...
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.30.93...
  2. SSF55681 (Class II ...)
  3. cd00496 (PheRS_alph...)

Residue annotation

  1. dimer interface cd...
  2. motif 1 cd00496
  3. active site cd00496
  4. motif 2 cd00496
  5. motif 3 cd00496

Protein sequence

>MCA_05697_1
MNSTKLIARWSNSLIQLGSRVALVSTKTNRCHYSSSTPSTVKVLGKEHSTDEWTNITPSIIELTTRKLHLNPSHPIGILR
KIFEDQLSKIGGYTFYNSFEPVVSKYENFDVLGFPEDHPGRSKTDTYYINKNTLLRTHTSAHEAECYQKCPTPGYLISAD
VYRRDTVDKTHYPAFHQMEGAYTWSRSEHPGESILEAIQKDLDSLPKPDIIVEDPNPPFHPERNPKEMSMSDKEVELVSA
HLKRTLELLLASVFNKAREAAFAAGIEDPDLMAPLKARWIEAYFPWTAPSYEIEIWWKGDWLEMCGCGVERQHVLTNVGI
EDKISWAFGVGLERMAMLLFGIPDIRLFWTKDPRFLSQFTEGTVTNFKPYSKYPGTLRDVAFWVPKDSEGIHENDIMSLV
RDIAGDLVENVSLQDEFVHPKTGRKSQCYRINYQSMDRSLTNDEINDLQDKFREQLAATYDIELR

GO term prediction

Biological Process

GO:0006432 phenylalanyl-tRNA aminoacylation
GO:0043039 tRNA aminoacylation

Molecular Function

GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004826 phenylalanine-tRNA ligase activity
GO:0005524 ATP binding

Cellular Component

GO:0005737 cytoplasm