Protein

MCA_05535_1

Length
478 amino acids

Gene name: DUS4

Description: tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]

Browser: contigD:1574960-1576397+

RNA-seq: read pairs 375, FPKM 9.7, percentile rank 25.4% (100% = highest expression)

Protein function

Annotation:DUS4tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]
KEGG:K05545DUS4 tRNA-dihydrouridine synthase 4 [EC:1.3.1.90]
EGGNOG:0PFQ9FG01258.1tRNA-dihydrouridine synthase 4
SGD closest match:S000004397DUS4tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]
CGD closest match:CAL0000191650DUS4tRNA-dihydrouridine synthase

Protein alignments

%idAln lengthE-value
MIA_00636_158.52%4462e-176MIA_00636_1
A0A0J9XJM3_GEOCN72.18%2488e-128tRNA-dihydrouridine synthase OS=Geotrichum candidum GN=BN980_GECA22s01605g PE=3 SV=1
A0A060T2F9_BLAAD70.90%2445e-114tRNA-dihydrouridine synthase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A01474g PE=3 SV=1
UniRef50_A0A1A0HAY863.35%2513e-104Dihydrouridine synthase n=1 Tax=Metschnikowia bicuspidata var. bicuspidata NRRL YB-4993 TaxID=869754 RepID=A0A1A0HAY8_9ASCO
A0A1E3PIM8_9ASCO64.23%2461e-105tRNA-dihydrouridine synthase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_51865 PE=3 SV=1
Q5A4Y0_CANAL59.18%2451e-100tRNA-dihydrouridine synthase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=DUS4 PE=3 SV=1
Q6C4K2_YARLI62.44%2213e-97tRNA-dihydrouridine synthase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E26059g PE=3 SV=1
A0A1E4TC28_9ASCO59.13%2308e-92tRNA-dihydrouridine synthase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_27333 PE=3 SV=1
A0A161HM33_9ASCO69.95%1932e-91tRNA-dihydrouridine synthase OS=Sugiyamaella lignohabitans GN=DUS4 PE=3 SV=1
DUS4_YEAST55.19%2413e-90tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DUS4 PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.7203
Predicted cleavage: 65

Protein family membership

Domains and repeats

  1. Domain
1 50 100 150 200 250 300 350 400 450 478

Detailed signature matches

  1. Family IPR001269
    tRNA-dihydrouridine synthase
    1. PF01207 (Dus)
  2. Domain IPR013785
    Aldolase-type TIM barrel
    1. G3DSA:3.20.20.70
  3. Domain IPR035587
    DUS-like, FMN-binding domain
    1. cd02801 (DUS_like_FMN)
  4. SITE IPR018517
    tRNA-dihydrouridine synthase, conserved site
    1. PS01136 (UPF0034)
Unintegrated signatures no IPR
Unintegrated signatures
  1. SSF51395 (FMN-linke...)

Residue annotation

  1. FMN binding site c...
  2. active site cd02801
  3. catalytic residues...
  4. substrate binding ...

Protein sequence

>MCA_05535_1
MSTNKYPLSPPVHLNIPPRDIKNHPVTVIKRINEKCHRPAFISGPMVRYSKLPFRELVRHHDTDIVYTPMILAREFVRNG
VARASDFSTNNKDAPVVAQFGANNEIDLVRAVSMILPYVDGIGLNCGCPIKDQVREGIGAALMTNLPNVVNMVKAVKSKF
GEQVCMEVKIRIHKDLNETVEFAKSVEKAGADYITVHGRLKPQRSSEACNFEAIKLIKSSVSIPVVANGDAFSVEDALRI
AHYTGVDGVMAARGILQNPAMFDIMAYRERRNYIKQLGEPVPGKFRNTLRINDEYTDFVDKIMEPVPILRQCEIESQTPE
LPVSQPTVDGKPAHILKAHLYNTTPWSSIERFWDIVTAYGLPYRVTQHHFSEFLDTTLSKTDKKSMNDTKNLVELLQWFD
ERFDLKRPNQPGAFEDREYPWKEEYLKAQELLNQEEASGQVKESKSTTDQLIAESNQPAYDQINIDIKNLQISPKIAS

GO term prediction

Biological Process

GO:0008033 tRNA processing
GO:0055114 oxidation-reduction process

Molecular Function

GO:0003824 catalytic activity
GO:0017150 tRNA dihydrouridine synthase activity
GO:0050660 flavin adenine dinucleotide binding

Cellular Component

None predicted.