Protein

MCA_05316_1

Length
548 amino acids

Gene name: MST1

Description: Threonine--tRNA ligase, mitochondrial

Browser: contigD:991214-992861-

RNA-seq: read pairs 1743, FPKM 39.2, percentile rank 59.9% (100% = highest expression)

Protein function

Annotation:MST1Threonine--tRNA ligase, mitochondrial
KEGG:K01868TARS threonyl-tRNA synthetase [EC:6.1.1.3]
EGGNOG:0PG6EFG00749.1threonyl-trna synthetase
SGD closest match:S000001677MST1Threonine--tRNA ligase, mitochondrial
CGD closest match:CAL0000183181MST1Threonine--tRNA ligase

Protein alignments

%idAln lengthE-value
MIA_05900_168.18%4840.0MIA_05900_1
A0A0J9XE33_GEOCN64.38%4800.0Similar to Saccharomyces cerevisiae YKL194C MST1 Mitochondrial threonyl-tRNA synthetase OS=Geotrichum candidum GN=BN980_GECA10s00670g PE=4 SV=1
A0A167FF71_9ASCO53.65%4790.0Threonine--tRNA ligase MST1 OS=Sugiyamaella lignohabitans GN=MST1 PE=4 SV=1
A0A060SYQ3_BLAAD53.72%4710.0ARAD1A18766p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A18766g PE=4 SV=1
A0A1E3PNP8_9ASCO50.10%4835e-168Mitochondrial threonyl-tRNA synthetase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_69720 PE=4 SV=1
UniRef50_A5DVX051.04%4802e-156Threonyl-tRNA synthetase, mitochondrial n=9 Tax=Saccharomycetales TaxID=4892 RepID=A5DVX0_LODEL
A0A1D8PCV0_CANAL48.33%4804e-157Threonine--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=MST1 PE=4 SV=1
Q6CHF9_YARLI45.68%4865e-153YALI0A09427p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_A09427g PE=4 SV=1
SYTM_YEAST45.64%4932e-149Threonine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MST1 PE=1 SV=2
A0A1E4TEK5_9ASCO43.76%4732e-139Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_24071 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9592
Predicted cleavage: 34

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 50 100 150 200 250 300 350 400 450 500 548

Detailed signature matches

  1. Family IPR002320
    Threonine-tRNA ligase, class IIa
    1. TIGR00418
    2. PR01047 (TRNASYNTHTHR)
  2. Domain IPR006195
    Aminoacyl-tRNA synthetase, class II
    1. PS50862 (AA_TRNA_LI...)
  3. Domain IPR002314
    Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)
    1. PF00587 (tRNA-synt_2b)
  4. Domain IPR033728
    Threonine-tRNA ligase catalytic core domain
    1. cd00771 (ThrRS_core)
  5. Domain IPR004154
    Anticodon-binding
    1. SSF52954 (Class II ...)
    2. G3DSA:3.40.50...
    3. PF03129 (HGTP_antic...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.30.93...
  2. SSF55681 (Class II ...)
  3. cd00860 (ThrRS_anti...)
  4. mobidb-lite (disord...)

Residue annotation

  1. active site cd00771
  2. dimer interface cd...
  3. motif 1 cd00771
  4. motif 2 cd00771
  5. motif 3 cd00771
  6. anticodon binding ...

Protein sequence

>MCA_05316_1
MLQANRSLRSSSRLYALSRQHLSRPIQSSLIKSHIQTKPFSQSVTTRKKQQDPSSSASPSSSVSSIATESKTIANKQKLY
VIDQLSPGSIFFLPNGTRIFNRLIEFMRAQQRCYGFEEVITPLIYKDDLWKTSGHWQHYKDDMFQVSANHHTSETPVEQQ
QQQSSSQITESNNNAQQKSPEEIDQELTQIYGLKPMNCPGHCVMFGKHDHSFRDLPIRFSDFSPLHRNETSGALSGLTRV
RKFHQDDGHIFCQPSQIKSEIKSCLNMIESSYSVFGLKQFRLALSTRPDSYAGELSVWDHAEQNLKEALEDSGKDWFINP
GDGAFYGPKIDILVKDNNGKEHQTATIQLDFQLPKNFNLKYVSGTPTEQQKQKQQQQEEEGKTEELLTPVLIHRAVFGSL
ERFMAILLDHYQGKWPFWLNPRQAIIIPVADRHKEYAFKVYSQLSGKYNFKYTTELNDSTERAQTSTSVLPALLHSRYFN
VDLSATDETVGAKTRRAISDGYNFIVVIGDKEVENNQIAVRSKDSRQTQLLTVEQLKDMFSTLEDTYQ

GO term prediction

Biological Process

GO:0006418 tRNA aminoacylation for protein translation
GO:0006435 threonyl-tRNA aminoacylation

Molecular Function

GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004829 threonine-tRNA ligase activity
GO:0005524 ATP binding

Cellular Component

GO:0005737 cytoplasm