Protein

MCA_05082_1

Length
390 amino acids

Browser: contigD:326353-327526+

RNA-seq: read pairs 4255, FPKM 134.5, percentile rank 83.5% (100% = highest expression)

Protein function

KEGG:K11517HAO (S)-2-hydroxy-acid oxidase [EC:1.1.3.15]
EGGNOG:0PKHE(S)-2-hydroxy-acid oxidase
SGD closest match:S000004518CYB2Cytochrome b2, mitochondrial
CGD closest match:CAL0000184748CYB2Cyb2p

Protein alignments

%idAln lengthE-value
MIA_03819_170.26%3900.0MIA_03819_1
A0A0J9X7A4_GEOCN60.82%3885e-173Similar to Saccharomyces cerevisiae YML054C CYB2 Cytochrome b2 (L-lactate cytochrome-c oxidoreductase) OS=Geotrichum candidum GN=BN980_GECA04s07677g PE=3 SV=1
A0A167EFD9_9ASCO58.97%3684e-159Cyb2p OS=Sugiyamaella lignohabitans GN=CYB2 PE=3 SV=1
UniRef50_A0A167EFD958.97%3681e-155Cyb2p n=3 Tax=Saccharomycetales TaxID=4892 RepID=A0A167EFD9_9ASCO
A0A060T411_BLAAD56.07%3876e-156ARAD1C44770p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C44770g PE=3 SV=1
Q6C9A7_YARLI54.97%3822e-149YALI0D12661p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_D12661g PE=3 SV=1
A0A1E3PKA0_9ASCO53.95%3806e-144FMN-dependent alpha-hydroxy acid dehydrogenase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_51131 PE=3 SV=1
A0A1E4T9K5_9ASCO53.03%3792e-142Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_146120 PE=3 SV=1
CYB2_YEAST33.51%3796e-49Cytochrome b2, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CYB2 PE=1 SV=1
Q5AKX8_CANAL31.18%3722e-47Cyb2p OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=CYB2 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0075

Protein family membership

Domains and repeats

  1. Domain
1 50 100 150 200 250 300 350 390

Detailed signature matches

  1. Family IPR012133
    Alpha-hydroxy acid dehydrogenase, FMN-dependent
    1. PS51349 (FMN_HYDROX...)
    2. cd02809 (alpha_hydr...)
    3. PIRSF000138 (Al-hdr...)
  2. Domain IPR013785
    Aldolase-type TIM barrel
    1. G3DSA:3.20.20.70
  3. Domain IPR000262
    FMN-dependent dehydrogenase
    1. PF01070 (FMN_dh)
  4. SITE IPR008259
    FMN-dependent alpha-hydroxy acid dehydrogenase, active site
    1. PS00557 (FMN_HYDROX...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. SSF51395 (FMN-linke...)

Residue annotation

  1. substrate binding ...
  2. active site cd02809
  3. FMN binding site c...
  4. putative catalytic...

Protein sequence

>MCA_05082_1
MEPYTSGPKHIYNLTDLENAALPKATKMVREFWQGGAGDMKTLKENMSVFDDYRIRTRVMVDVSEIDMRPTTKIFGKDYA
VPIGFAPAAFHQFADDDGEVATARAAKAKNWLMGLSSYSTKSLEDVKQAGGENVNVLQLYVFQNRETTKKLVQRAEKAGY
KAIALTVDTPVVAVRPADVYNEFKLPGHIKMGNFDSFGHGVIDNPVSPNSLARQAVTVGGEKSEQAKKSNALDPTLTWDE
TIKWLRSITKLEIWVKGVTSAEDAEAAVNAGVDGIWVSNHGGRQLDSALATLDSLPEVVAAVNKRVPVHVDGGFRRGADV
FKALALGADFVHVGRPVLYGLLYDGQKGVELMAKILEDELKYCMMFAGTRNIAEIQRHKLVRKAHHLERL

GO term prediction

Biological Process

GO:0055114 oxidation-reduction process

Molecular Function

GO:0003824 catalytic activity
GO:0010181 FMN binding
GO:0016491 oxidoreductase activity

Cellular Component

None predicted.