Protein

MCA_05045_1

Length
775 amino acids

Description: Cysteine--tRNA ligase

Browser: contigD:213140-215468+

RNA-seq: read pairs 2647, FPKM 42.1, percentile rank 61.5% (100% = highest expression)

Protein function

Annotation:Cysteine--tRNA ligase
KEGG:K01883CARS cysteinyl-tRNA synthetase [EC:6.1.1.16]
EGGNOG:0PGE0FG05963.1Cysteinyl-tRNA synthetase
SGD closest match:S000005191YNL247WCysteine--tRNA ligase
CGD closest match:CAL0000188986orf19.4931Cysteine--tRNA ligase

Protein alignments

%idAln lengthE-value
MIA_03806_169.38%7610.0MIA_03806_1
A0A0J9YHG0_GEOCN63.73%7500.0Similar to Saccharomyces cerevisiae YNL247W Cysteinyl-tRNA synthetase OS=Geotrichum candidum GN=BN980_GECA01s03937g PE=3 SV=1
A0A060TJJ8_BLAAD59.23%7580.0ARAD1D47366p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D47366g PE=3 SV=1
A0A1E3PRL7_9ASCO57.56%7610.0Cysteinyl-tRNA synthetase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_48587 PE=3 SV=1
A0A161HKG7_9ASCO55.94%7580.0Cysteine--tRNA ligase OS=Sugiyamaella lignohabitans GN=AWJ20_1780 PE=3 SV=1
SYC_YEAST54.02%7830.0Cysteine--tRNA ligase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YNL247W PE=1 SV=1
UniRef50_P5385254.02%7830.0Cysteine--tRNA ligase n=84 Tax=Saccharomycetales TaxID=4892 RepID=SYC_YEAST
Q6C909_YARLI55.89%7550.0YALI0D15268p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_D15268g PE=3 SV=1
Q5AL46_CANAL52.90%7580.0Cysteine--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.4931 PE=3 SV=1
A0A1E4TA91_9ASCO49.51%7150.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_57811 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9589
Predicted cleavage: 20

Protein family membership

Domains and repeats

  1. Domain
1 100 200 300 400 500 600 700 775

Detailed signature matches

  1. Family IPR024909
    Cysteinyl-tRNA synthetase/mycothiol ligase
    1. PR00983 (TRNASYNTHCYS)
  2. Family IPR015803
    Cysteine-tRNA ligase
    1. MF_00041 (Cys_tRNA_...)
    2. TIGR00435
  3. Domain IPR014729
    Rossmann-like alpha/beta/alpha sandwich fold
    1. G3DSA:3.40.50...
  4. Domain IPR032678
    tRNA synthetases class I, catalytic domain
    1. PF01406 (tRNA-synt_1e)
    2. cd00672 (CysRS_core)
  5. Domain IPR009080
    Aminoacyl-tRNA synthetase, class Ia, anticodon-binding
    1. SSF47323 (Anticodon...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:1.20.12...
  3. SSF52374 (Nucleotid...)

Residue annotation

  1. active site cd00672
  2. HIGH motif cd00672
  3. KMSKS motif cd00672

Protein sequence

>MCA_05045_1
MSFLINHLKRPFTKLNPTIIKCTMSTAQPKWYAPEPIKASPKRKPLKLYNSLTRSAEPFYPISDDKVTWYSCGPTVYDSS
HLGHARNYVSIDINRRIIQDYFGYDVLFVQNVTDIDDKIIIKARQVYLFDQYKNKWLASSPNTPSDELVADIKLAWAKYV
SSNLAKFNVPSILEDFADWKQKSTEKFATYATEDPKFNMHLQAVSESADALNSSSKTEELLNASKSILSPYLDAKDGSSV
TDPKIFRDLAAYWEVKFDEDMRRLNVLPPNVTTRVSEYIPENIAFIEKIVSNGFGYKTDDGSVYFDTTAFEKSGRHVYAK
LQPWNRGNKDLIDEGEGSLSSKTGGKKSNADFALWKASKPGEPSWDSPWGKGRPGWHIECSVMASEILGPEIDIHTGGID
LAFPHHDNELAQSEACHDNHQWVNYFLHTGHLHIEGQKMSKSLKNFITIKEALQMFTPRQLRLAFAMQQWNTPMDLKINL
SEVKSFESTVSKYFGRVKALLRENEDAIKNGVNVPKKLTNKEKQLWQDLTDTKVAVHNAFADNLSVPVALSAIDKLIQKV
NIYLGEVENNFLEEVLYSTTTWITSIFRILGFKVNSDGLGWSSEEQGSSASSEDVILPYLKVLSTFRDTVRTKAIEKAPY
SEFLQATDKIRNEDLIDLGVSLDDRTDGPALIKFLDAGEKQAIIKQREEKEAREAAKKAKKAELAKAEELKRQKEKELAS
IKPEDLFKNLEDVAEWDESGIPSVLKDGNAVSKSQKKKYVKQMEQHKKLYLKYNS

GO term prediction

Biological Process

GO:0006418 tRNA aminoacylation for protein translation
GO:0006423 cysteinyl-tRNA aminoacylation

Molecular Function

GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004817 cysteine-tRNA ligase activity
GO:0005524 ATP binding

Cellular Component

None predicted.