Protein

MCA_05040_1

Length
489 amino acids

Gene name: FUM1

Description: Fumarate hydratase, mitochondrial

Browser: contigD:202312-203938-

RNA-seq: read pairs 22413, FPKM 565.2, percentile rank 94.7% (100% = highest expression)

Protein function

Annotation:FUM1Fumarate hydratase, mitochondrial
KEGG:K01679E4.2.1.2B fumarate hydratase, class II [EC:4.2.1.2]
EGGNOG:0PIADFUM1Fumarate hydratase
SGD closest match:S000006183FUM1Fumarate hydratase, mitochondrial
CGD closest match:CAL0000174516FUM12Fum12p

Protein alignments

%idAln lengthE-value
A0A0J9XBD4_GEOCN86.50%4890.0Similar to Saccharomyces cerevisiae YPL262W FUM1 Fumarase converts fumaric acid to L-malic acid in the TCA cycle OS=Geotrichum candidum GN=BN980_GECA08s00263g PE=3 SV=1
MIA_04836_187.35%4900.0MIA_04836_1
A0A167FFF8_9ASCO88.96%4710.0Fumarase FUM1 OS=Sugiyamaella lignohabitans GN=FUM1 PE=3 SV=1
A0A060T1D3_BLAAD86.86%4720.0ARAD1C23848p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C23848g PE=3 SV=1
A0A1E3PJF8_9ASCO84.08%4710.0Fumarate hydratase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_82587 PE=3 SV=1
Q6CCT2_YARLI82.38%4710.0YALI0C06776p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_C06776g PE=3 SV=1
A0A1E4TD34_9ASCO80.51%4720.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_141980 PE=3 SV=1
A0A1D8PKV4_CANAL76.10%4560.0Fum12p OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=FUM12 PE=3 SV=1
FUMH_YEAST75.05%4610.0Fumarate hydratase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=FUM1 PE=1 SV=2
UniRef50_V5GAF372.09%4730.0Fumarate hydratase class II n=4 Tax=Eurotiales TaxID=5042 RepID=V5GAF3_BYSSN

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9807
Predicted cleavage: 32

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 50 100 150 200 250 300 350 400 450 489

Detailed signature matches

  1. Family IPR000362
    Fumarate lyase family
    1. PR00149 (FUMRATELYASE)
  2. Family IPR005677
    Fumarate hydratase, class II
    1. cd01362 (Fumarase_c...)
    2. TIGR00979
    3. MF_00743 (FumaraseC)
  3. Domain IPR024083
    Fumarase/histidase, N-terminal
    1. G3DSA:1.10.27...
  4. Domain IPR008948
    L-Aspartase-like
    1. SSF48557 (L-asparta...)
  5. Domain IPR022761
    Fumarate lyase, N-terminal
    1. PF00206 (Lyase_1)
  6. Domain IPR018951
    Fumarase C, C-terminal
    1. PF10415 (FumaraseC_C)
  7. SITE IPR020557
    Fumarate lyase, conserved site
    1. PS00163 (FUMARATE_L...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:1.10.40.30
  2. G3DSA:1.20.20...

Residue annotation

  1. active site cd01362
  2. tetramer interface...

Protein sequence

>MCA_05040_1
MIRTFASSKSVAAASVRRNFSVSAYLLGKRIETDAFGEIEVEDSRYWGAQTQRSLQNFEIGGNREKLPEPLIKAFGILKK
AAATVNMTYGLDAKVGEAIQQAAQEVIDGKLTDHFPLVVFQTGSGTQSNMNSNEVISNRAIELLGGKLGTKTPVHPNDHV
NMSQSSNDTFPTVMHIAAVLQITQHLLPNLEALHKAVDDKAKEFSHIIKIGRTHLQDATPLTLGQEFSGYAAQIQNGIDR
IKDTLPRLRYLAQGGTAVGTGLNTRKGFDKAIADTVTKLTGEQFYTAPNKFEALAAHDAFVEASGALNTVAASLFKIAND
IRYLGSGPRCGYGELHLPENEPGSSIMPGKVNPTQNEALTMVCTQVFGNHACITYAGASGQFELNVFKPVMASNLLSSIR
LLGDAARSFRIHCVEGIVANEKRIEQLMRESLMLVTALNPKIGYDKASKVAKNAHKKGITLKESALELGVLTSEEFDQWV
RPEEMIGPK

GO term prediction

Biological Process

GO:0006099 tricarboxylic acid cycle
GO:0006106 fumarate metabolic process

Molecular Function

GO:0003824 catalytic activity
GO:0004333 fumarate hydratase activity
GO:0016829 lyase activity

Cellular Component

GO:0045239 tricarboxylic acid cycle enzyme complex