MCA_04387_1

Protein

Length

1,741 amino acids

Gene name: DNF3

Description: Trans-golgi network aminophospholipid translocase (flippase)

Browser: contigC:2851041-2856397-

RNA-seq: read pairs 5823, FPKM 41.3, percentile rank 61.0% (100% = highest expression)

Protein function

Annotation:		DNF3	Trans-golgi network aminophospholipid translocase (flippase)
KEGG:	K01530	E3.6.3.1	phospholipid-translocating ATPase [EC:3.6.3.1]
EGGNOG:	0QE8D	PGUG_02494	P-type ATPase
SGD closest match:	S000004772	DNF3	Probable phospholipid-transporting ATPase DNF3
CGD closest match:	CAL0000200114	orf19.2680	Phospholipid-transporting ATPase

Protein alignments

	%id	Aln length
MIA_02044_1	67.95%	1694	MIA_02044_1
A0A0J9XAS8_GEOCN	62.14%	1664	Phospholipid-transporting ATPase OS=Geotrichum candidum GN=BN980_GECA06s02023g PE=3 SV=1
UniRef50_A0A0J9XAS8	62.14%	1664	Phospholipid-transporting ATPase n=2 Tax=Saccharomycetales TaxID=4892 RepID=A0A0J9XAS8_GEOCN
A0A060T785_BLAAD	58.55%	1438	Phospholipid-transporting ATPase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D01364g PE=3 SV=1
A0A1E4THZ1_9ASCO	49.69%	1435	Phospholipid-transporting ATPase (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_15651 PE=3 SV=1
A0A167CC88_9ASCO	59.08%	958	Phospholipid-transporting ATPase OS=Sugiyamaella lignohabitans GN=DNF3 PE=3 SV=1
A0A1E3PH37_9ASCO	54.35%	955	Phospholipid-transporting ATPase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_83556 PE=3 SV=1
B5FVH1_YARLI	51.07%	979	Phospholipid-transporting ATPase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E04169g PE=3 SV=1
A0A1D8PLQ7_CANAL	50.65%	843	Phospholipid-transporting ATPase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.2680 PE=3 SV=1
ATC8_YEAST	44.95%	950	Probable phospholipid-transporting ATPase DNF3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DNF3 PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0034

Protein family membership

P-type ATPase (IPR001757)
- P-type ATPase, subfamily IV (IPR006539)

Domains and repeats

Domain
270 - 327
P-type ATPase, N-terminal (IPR032631)
400 - 460
P-type ATPase, A domain (IPR008250)
496 - 534
P-type ATPase, A domain (IPR008250)
535 - 1,374
HAD-like domain (IPR023214)
P-type ATPase, cytoplasmic domain N (IPR023299)
Domain
274 - 361
P-type ATPase, transmembrane domain (IPR023298)
539 - 656
P-type ATPase, transmembrane domain (IPR023298)
1,367 - 1,594
P-type ATPase, transmembrane domain (IPR023298)
Domain
1,346 - 1,597
P-type ATPase, C-terminal (IPR032630)

1 200 400 600 800 1000 1200 1400 1600 1741

Detailed signature matches

IPR001757

P-type ATPase
1. TIGR01494
  
  1,289 - 1,402
  
  TIGRFAMs
  TIGR01494 (TIGR01494)
  
  1,172 - 1,222
  
  TIGRFAMs
  TIGR01494 (TIGR01494)
IPR006539

P-type ATPase, subfamily IV
1. TIGR01652
  
  1,104 - 1,604
  
  TIGRFAMs
  TIGR01652 (TIGR01652)
IPR032631

P-type ATPase, N-terminal
1. PF16209 (PhoLip_ATP...)
  
  270 - 327
  
  Pfam
  PF16209 (PhoLip_ATPase_N)
IPR023298

P-type ATPase, transmembrane domain
1. SSF81665 (Calcium A...)
  
  539 - 656
  
  SUPERFAMILY
  SSF81665 (Calcium ATPase, transmembrane domain M)
  
  274 - 361
  
  SUPERFAMILY
  SSF81665 (Calcium ATPase, transmembrane domain M)
  
  1,367 - 1,594
  
  SUPERFAMILY
  SSF81665 (Calcium ATPase, transmembrane domain M)
IPR008250

P-type ATPase, A domain
1. SSF81653 (Calcium A...)
  
  400 - 460
  
  SUPERFAMILY
  SSF81653 (Calcium ATPase, transduction domain A)
  
  496 - 534
  
  SUPERFAMILY
  SSF81653 (Calcium ATPase, transduction domain A)
IPR023214

HAD-like domain
1. SSF56784 (HAD-like)
  
  1,289 - 1,374
  
  SUPERFAMILY
  SSF56784 (HAD-like)
  
  1,115 - 1,237
  
  SUPERFAMILY
  SSF56784 (HAD-like)
  
  657 - 673
  
  SUPERFAMILY
  SSF56784 (HAD-like)
2. G3DSA:3.40.50...
  
  1,176 - 1,359
  
  GENE3D
  G3DSA:3.40.50.1000 (G3DSA:3.40.50.1000)
IPR023299

P-type ATPase, cytoplasmic domain N
1. SSF81660 (Metal cat...)
  
  866 - 960
  
  SUPERFAMILY
  SSF81660 (Metal cation-transporting ATPase, ATP-binding domain N)
2. G3DSA:3.40.11...
  
  535 - 1,175
  
  GENE3D
  G3DSA:3.40.1110.10 (G3DSA:3.40.1110.10)
IPR032630

P-type ATPase, C-terminal
1. PF16212 (PhoLip_ATP...)
  
  1,346 - 1,597
  
  Pfam
  PF16212 (PhoLip_ATPase_C)
IPR018303

P-type ATPase, phosphorylation site
1. PS00154 (ATPASE_E1_E2)
  
  664 - 670
  
  PROSITE patterns
  PS00154 (ATPASE_E1_E2)

no IPR

Unintegrated signatures

CYTOPLASMIC_D... (C...)

1 - 300

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)

1,510 - 1,520

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)

344 - 554

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)

1,588 - 1,741

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)

619 - 1,456

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)
Coil

1,021 - 1,041

COILS
Coil (Coil)

1,725 - 1,741

COILS
Coil (Coil)
G3DSA:2.70.15...

309 - 534

GENE3D
G3DSA:2.70.150.10 (G3DSA:2.70.150.10)
NON_CYTOPLASM... (N...)

1,481 - 1,491

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)

1,546 - 1,566

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)

579 - 597

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)

319 - 323

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
PF00122 (E1-E2_ATPase)

400 - 595

Pfam
PF00122 (E1-E2_ATPase)
PF00702 (Hydrolase)

1,118 - 1,263

Pfam
PF00702 (Hydrolase)
PF13246 (Cation_ATPase)

870 - 955

Pfam
PF13246 (Cation_ATPase)
PR00119 (CATATPASE)

491 - 505

PRINTS
PR00119 (CATATPASE)

1,197 - 1,207

PRINTS
PR00119 (CATATPASE)

1,318 - 1,337

PRINTS
PR00119 (CATATPASE)
SFLDF00027 (p-type_...)

645 - 1,371

SFLD
SFLDF00027 (p-type_atpase)
SFLDG00002 (C1.7:_P...)

645 - 1,371

SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)
TMhelix

1,457 - 1,479

TMHMM
TMhelix (TMhelix)

1,492 - 1,514

TMHMM
TMhelix (TMhelix)

1,524 - 1,546

TMHMM
TMhelix (TMhelix)

595 - 617

TMHMM
TMhelix (TMhelix)

1,559 - 1,581

TMHMM
TMhelix (TMhelix)

555 - 574

TMHMM
TMhelix (TMhelix)
TRANSMEMBRANE (Tran...)

598 - 618

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

1,492 - 1,509

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

301 - 318

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

324 - 343

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

555 - 578

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

1,457 - 1,480

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

1,521 - 1,545

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

1,567 - 1,587

PHOBIUS
TRANSMEMBRANE (Transmembrane region)
cd02073 (P-type_ATP...)

276 - 1,478

CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)
mobidb-lite (disord...)

1 - 97

MobiDB Lite
mobidb-lite (disorder_prediction)

173 - 212

MobiDB Lite
mobidb-lite (disorder_prediction)

1,655 - 1,741

MobiDB Lite
mobidb-lite (disorder_prediction)

137 - 158

MobiDB Lite
mobidb-lite (disorder_prediction)

720 - 806

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

phosphorylation si...

664D

phosphorylation site
664D
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)
P-type ATPase sign...

664D

P-type ATPase signature motif
664D, 665K, 666T, 667G, 668T, 669L, 670T
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

665K

P-type ATPase signature motif
664D, 665K, 666T, 667G, 668T, 669L, 670T
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

666T

P-type ATPase signature motif
664D, 665K, 666T, 667G, 668T, 669L, 670T
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

667G

P-type ATPase signature motif
664D, 665K, 666T, 667G, 668T, 669L, 670T
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

668T

P-type ATPase signature motif
664D, 665K, 666T, 667G, 668T, 669L, 670T
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

669L

P-type ATPase signature motif
664D, 665K, 666T, 667G, 668T, 669L, 670T
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

670T

P-type ATPase signature motif
664D, 665K, 666T, 667G, 668T, 669L, 670T
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)
putative ATP bindi...

664D

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

665K

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

666T

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

923F

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

946K

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

947G

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

948A

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,158K

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,205T

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,206G

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,207D

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,293R

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,296P

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,299K

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,320D

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,323N

putative ATP binding site
664D, 665K, 666T, 923F, 946K, 947G, 948A, 1,158K, 1,205T, 1,206G, 1,207D, 1,293R, 1,296P, 1,299K, 1,320D, 1,323N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)
SFLDF00027

664D

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDF00027 (p-type_atpase)

665K

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDF00027 (p-type_atpase)

666T

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDF00027 (p-type_atpase)

667G

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDF00027 (p-type_atpase)

668T

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDF00027 (p-type_atpase)

1,205T

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDF00027 (p-type_atpase)

1,299K

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDF00027 (p-type_atpase)

1,320D

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDF00027 (p-type_atpase)

1,324D

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDF00027 (p-type_atpase)
SFLDG00002

664D

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

665K

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

666T

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

667G

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

668T

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

1,205T

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

1,299K

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

1,320D

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

1,324D

SFLD_Res01
664D, 665K, 666T, 667G, 668T, 1,205T, 1,299K, 1,320D, 1,324D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

Protein sequence

>MCA_04387_1
MGNKKDKNPGIPMSSSSSVVSLNTLPETSSQAPSNANALSQATNSAFESEDNQNQMPSYPGIPYDQSNVSNPSLSSNPNV
EGSHPDTPMYSRSTHRRIPSVTTENITPQIHNRSNSLRSTMFNRNAKLSTSEPKYENIEGANHNDQDSSTPGTYFGPNSM
DIRQEHIFPYDSASTNQSVNTGQGQHNPHHKETSQMPDRSPTFYNDPSTKNPTNFKQKLQDVGVRGRFKTAISKTRDFIL
RVKRPPPSKGGRVIPVGIENDRPLLIDNRTNKPYCDNLITSSIYTYYNFLPRQLVAQFSKLANVYFLAVSIMQMIPSWST
TGTYTTIIPLLVFISISMGREGFDDWRRHRQDKEENNRTVKVARLNSSRISPVFSTSTTNLRTVATSSSSVDSATEPLVY
EEIEWKKLRVGDIIKLEQNDWVPADILILNSKGLGNIAYIETMALDGETNLKTREPLPEFVKKCSTPEDLSSFKCTVTTE
DPNIDLYNFEGTASIDGETFPLSSSHIVYRGSILRNTPSIHGLVVFSGEETKIRMNAIQNPRTKAPRLQQLVNKIVVFMV
GVVLFLAVFCTVAAKIMYNNEGKKYWYLRGIEVGVVPNLMGFIIMYNTLIPLSLYVSMEIVKVVQMLMLQYDVDMYHEPT
DTPMEAHTATINEELGQVSYIFSDKTGTLTENEMVFRKLSVGGYSWIHDLDIQMENQEDGEKLYHRVTQENKLALEQRKI
STQNAGAASRPSNLSRLSRPSTDQILQQVVSLPRKSTSRPSNSRPSNPRKSNATMRPSGLTAGRPSTNSLARTSSVRSVW
RSTAAPTKEQNIRSTLELLQYLLVHPNSVYARKAKFFILSIALCHTCVPDIDVSRNESGETLIENLEYQAASPDELALID
AARDMGFIMVDRQHKTMTIRTYPNGFDQDPVDELYEILDVVEFSSARKRMSILVRFPDGRVCVFCKGADNIILERLRLSD
IAAEKVVEINRQTAIRKTAEAEMVVARNSVSNTGGIQGPRRSITGDRRSIAIDPRDVLGSVDDYLQQHANVEEEIEEIAE
VSRRSMSLAARKRYGDESAISRTSAGNPSQSAATRLSLEIEAAVAPQKDDEFEEFSPDDRLVLNDSYVIEQTLEHIEEFS
TEGLRTLLYSYRFMGEEEYTTWAKDYSEAKTSLTDRQKKIEDVAERIEVDLDLCGATAIEDKLQDGVPEAIDKLRRAGIK
LWMLTGDKRETAINIGYSCRLIKDYSTVIVLRSDEDDVAAKIAVAMQELERGNVAHCVVVVDGATLAGIEQDMTLMTLFI
ELGVKANSVICCRASPSQKAAMVTAVRKKVKSSITLAIGDGANDIAMIQSADVGIGITGREGLQAARSSDYAIAQFRFLL
KLLLVHGRWNYVRTCKYILGTFYKEIFFYLTQAVYQRNVMFTGSSLYETWSLSMFNTLFTSLPVICIGIMEKDLKASTLI
AVPELYKKGQNNEGFDFWIFMGWIIIGASQSVLVSFITYYVYGYNLLKENSVFPFGVMLFTTVVVMCTVKLQLLEMHSKT
FVNFAVIAICVGGWFIWNMFLSYIYGNSPSKIYNVHDAIFNYWGHDLAWWMILLCCIVVSVVLDMMLQSFRVVVRPTDTD
TFQELEQYPPVHRRLQKEAFSELQQGWRTGKFSNNPDAFAYVSGEMEYDYENNRYRPASSMTNRDSLTSSNSSHGYSNRT
SQHGASTDFNLEELDIMKPKRKRDKIKQKLRFAPQPTTLDDERAIQEILRRRQQETDEAER

GO term prediction

Biological Process

GO:0015914 phospholipid transport

Molecular Function

GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0004012 phospholipid-translocating ATPase activity
GO:0005524 ATP binding

Cellular Component

GO:0016021 integral component of membrane

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Entry type

Status

Per-residue features