Protein

MCA_03740_1

Length
716 amino acids

Gene name: THS1

Description: Threonine--tRNA ligase, cytoplasmic

Browser: contigC:943945-946197-

RNA-seq: read pairs 10848, FPKM 186.9, percentile rank 87.3% (100% = highest expression)

Protein function

Annotation:THS1Threonine--tRNA ligase, cytoplasmic
KEGG:K01868TARS threonyl-tRNA synthetase [EC:6.1.1.3]
EGGNOG:0PFEMTHS1threonyl-trna synthetase
SGD closest match:S000001340THS1Threonine--tRNA ligase, cytoplasmic
CGD closest match:CAL0000177380THS1Threonine--tRNA ligase

Protein alignments

%idAln lengthE-value
MIA_01502_187.01%7160.0MIA_01502_1
A0A0J9X941_GEOCN81.50%6920.0Similar to Saccharomyces cerevisiae YIL078W THS1 Threonyl-tRNA synthetase OS=Geotrichum candidum GN=BN980_GECA05s05686g PE=3 SV=1
A0A167EEC9_9ASCO77.76%6610.0Threonine--tRNA ligase THS1 OS=Sugiyamaella lignohabitans GN=THS1 PE=3 SV=1
A0A060T7H9_BLAAD73.59%6930.0ARAD1B23980p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B23980g PE=3 SV=1
Q6C234_YARLI71.66%6740.0YALI0F11231p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F11231g PE=3 SV=1
A0A1E3PS40_9ASCO72.84%6590.0Threonyl-tRNA synthetase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_50069 PE=3 SV=1
SYTC_YEAST70.85%6930.0Threonine--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=THS1 PE=1 SV=2
UniRef50_P0480170.85%6930.0Threonine--tRNA ligase, cytoplasmic n=222 Tax=Eukaryota TaxID=2759 RepID=SYTC_YEAST
A0A1D8PMV9_CANAL70.69%6620.0Threonine--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=THS1 PE=3 SV=1
A0A1E4T9K4_9ASCO64.21%6930.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_33015 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0422

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 600 716

Detailed signature matches

  1. Family IPR002320
    Threonine-tRNA ligase, class IIa
    1. TIGR00418
    2. MF_00184 (Thr_tRNA_...)
    3. PR01047 (TRNASYNTHTHR)
  2. Domain IPR012675
    Beta-grasp domain
    1. G3DSA:3.10.20.30
  3. Domain IPR012676
    TGS-like
    1. SSF81271 (TGS-like)
  4. Domain IPR004095
    TGS
    1. PF02824 (TGS)
  5. Domain IPR018163
    Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain
    1. SSF55186 (ThrRS/Ala...)
  6. Domain IPR012947
    Threonyl/alanyl tRNA synthetase, SAD
    1. PF07973 (tRNA_SAD)
    2. SM00863 (tRNA_SAD_4)
  7. Domain IPR006195
    Aminoacyl-tRNA synthetase, class II
    1. PS50862 (AA_TRNA_LI...)
  8. Domain IPR002314
    Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)
    1. PF00587 (tRNA-synt_2b)
  9. Domain IPR033728
    Threonine-tRNA ligase catalytic core domain
    1. cd00771 (ThrRS_core)
  10. Domain IPR004154
    Anticodon-binding
    1. SSF52954 (Class II ...)
    2. G3DSA:3.40.50...
    3. PF03129 (HGTP_antic...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:3.30.93...
  3. G3DSA:3.30.98...
  4. SSF55681 (Class II ...)
  5. cd00860 (ThrRS_anti...)
  6. cd01667 (TGS_ThrRS_N)

Residue annotation

  1. active site cd00771
  2. dimer interface cd...
  3. motif 1 cd00771
  4. motif 2 cd00771
  5. motif 3 cd00771
  6. anticodon binding ...

Protein sequence

>MCA_03740_1
MCPPTEDSKVADLAKKVEKVDMKSKSQGFQSKADFVEERLKFYEELKQKSEAELAKKPREPIKITLKDGTVIDGTSWETT
PFQIAKDISKSLLDRVFISKVNGELWDLERPFEGDATLELLDFEDDEAKKVFWHSSAHVLGEATENVFGAHLCFGPPTDD
GFFYDMSIDNGEKAVAASDFPALETNAKAAIKQKQKFERLLVSKEDLLKMFHYNKYKKALIESKVPDGTMTTVYRCGPLI
DLCRGPHVPHTGRIKAFKLLKNSASYFLGDANNDSLQRIYGVSFPDKKLMDAHLKFLEEAAKRDHRKIGREQELFMFHEM
SPGSCFWLPHGTRIYNTLNAFLRDEYRDRGYEEVITPNMYNANLWKTSGHWENYKENMFTMEVEKDTFGLKPMNCPGHCL
MFKARERSYRELPWRVADFGVIHRNEFSGALSGLTRVRRFQQDDAHIFCRQDQIETEIVGVFNFLEKVYGIFGFEFKMEL
STRPEKYVGDIETWNDAESKLTNALNSFGRKWEINPGDGAFYGPKIDIMISDALKRWHQCATIQLDFQLPRRFELEYRAA
DGTAAATAEGESERYVHPVMIHRAIYGSFERMIGILTEHFFGKWPFWLSPRQVLVIPVGVKFNDYAQKVRDTLWEAGLFA
DVDLGPNTLQKKVRTGQLQQYNFIFIVGDEEEKSGSVNVRNRDDTSKQQRGIPFQLDEIISKLLALKEKKSLTHDL

GO term prediction

Biological Process

GO:0006418 tRNA aminoacylation for protein translation
GO:0006435 threonyl-tRNA aminoacylation
GO:0043039 tRNA aminoacylation

Molecular Function

GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004829 threonine-tRNA ligase activity
GO:0005524 ATP binding
GO:0016876 ligase activity, forming aminoacyl-tRNA and related compounds

Cellular Component

GO:0005737 cytoplasm