MCA_03385_1

Protein

Length

1,527 amino acids

Gene name: TRM3

Description: tRNA (guanosine(18)-2'-O)-methyltransferase

Browser: contigB:4276235-4280819-

RNA-seq: read pairs 597, FPKM 4.8, percentile rank 17.1% (100% = highest expression)

Protein function

Annotation:		TRM3	tRNA (guanosine(18)-2'-O)-methyltransferase
KEGG:	K15333	TRM3	tRNA guanosine-2'-O-methyltransferase [EC:2.1.1.34]
EGGNOG:	0PIFH	PGUG_04534	SpoU rRNA Methylase family
SGD closest match:	S000002270	TRM3	tRNA (guanosine(18)-2'-O)-methyltransferase
CGD closest match:	CAL0000195334	orf19.5038	tRNA (Guanosine(18)-2'-O)-methyltransferase

Protein alignments

	%id	Aln length	E-value
MIA_03909_1	41.46%	1546	0.0	MIA_03909_1
A0A0J9XAQ6_GEOCN	36.86%	1549	0.0	Similar to Saccharomyces cerevisiae YDL112W TRM3 2'-O-ribose methyltransferase OS=Geotrichum candidum GN=BN980_GECA08s01033g PE=4 SV=1
UniRef50_A0A0J9XAQ6	36.86%	1549	0.0	Similar to Saccharomyces cerevisiae YDL112W TRM3 2'-O-ribose methyltransferase n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XAQ6_GEOCN
A0A1E3PIA2_9ASCO	37.39%	773	3e-126	Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_51688 PE=4 SV=1
A0A060SXQ6_BLAAD	34.01%	794	1e-122	ARAD1A06446p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A06446g PE=4 SV=1
Q6C9I3_YARLI	33.29%	796	1e-107	YALI0D10989p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_D10989g PE=4 SV=1
A0A167DLG5_9ASCO	33.55%	772	5e-103	Trm3p OS=Sugiyamaella lignohabitans GN=TRM3 PE=4 SV=1
A0A1D8PLX4_CANAL	32.80%	744	9e-91	tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.5038 PE=4 SV=1
TRM3_YEAST	39.02%	469	2e-83	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TRM3 PE=1 SV=1
A0A1E4TE41_9ASCO	36.08%	474	2e-80	Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_14145 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1087

Protein family membership

None predicted.

Domains and repeats

Domain
2 - 552
Armadillo-type fold (IPR016024)
Armadillo-like helical (IPR011989)
622 - 1,264
Armadillo-type fold (IPR016024)
Armadillo-like helical (IPR011989)
1,366 - 1,527
Alpha/beta knot methyltransferases (IPR029028)
tRNA (guanine-N1-)-methyltransferase, N-terminal (IPR029026)
tRNA/rRNA methyltransferase, SpoU type (IPR001537)

1 200 400 600 800 1000 1200 1400 1527

Detailed signature matches

IPR016024

Armadillo-type fold
1. SSF48371 (ARM repeat)
  
  387 - 452
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  11 - 286
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  839 - 900
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  1,021 - 1,083
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  717 - 800
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
IPR011989

Armadillo-like helical
1. G3DSA:1.25.10.10
  
  2 - 552
  
  GENE3D
  G3DSA:1.25.10.10 (G3DSA:1.25.10.10)
  
  622 - 1,264
  
  GENE3D
  G3DSA:1.25.10.10 (G3DSA:1.25.10.10)
IPR029028

Alpha/beta knot methyltransferases
1. SSF75217 (alpha/bet...)
  
  1,370 - 1,525
  
  SUPERFAMILY
  SSF75217 (alpha/beta knot)
IPR029026

tRNA (guanine-N1-)-methyltransferase, N-terminal
1. G3DSA:3.40.12...
  
  1,366 - 1,527
  
  GENE3D
  G3DSA:3.40.1280.10 (G3DSA:3.40.1280.10)
IPR001537

tRNA/rRNA methyltransferase, SpoU type
1. PF00588 (SpoU_methy...)
  
  1,376 - 1,518
  
  Pfam
  PF00588 (SpoU_methylase)

no IPR

Unintegrated signatures

Coil

1,527 - 1,527

COILS
Coil (Coil)
mobidb-lite (disord...)

1,306 - 1,342

MobiDB Lite
mobidb-lite (disorder_prediction)

Protein sequence

>MCA_03385_1
MSSAVSSLISSLPLENRKALVSQLRDQIIKSNDEKIQHESIAALSEVIPLIDSEDDLKETYSALTSYIRQSLKDQASKLV
PIILQKIPALRVEFISDCLSIFHQYISQQESQYLRDAFSNYISFSTSSAYDENSDNIVITYIDVLYKVLLQLSPSDIKQC
CSSKLADYLLILHCFNNTTEISTLSQKAFGALSRGVDFLTFDLWWKSIVTICQNENVDSTNGYILWLRAVNGTPMLNKSS
LKNLQALFDDPLYWQLLINGLLDSSFDIRKYTLHIISQSVFKIDHNIDIETMYWDIKKRNLHLLEWERYSTLVNIVSIDT
SIYQAGDSTNDLIRLIGKDSYIPKPWSRCLLATGLKSSADAIRQFVAGVVLSLTTKDMGIFLDGEEFLTDVLLPQLMMAY
NFTVKKQSERIYKCPFAEQFASFIESIFNFLDDDKARSLCNCLLQFLYEKRQSFDPARVILARGIEQGLRNRAILSQSNL
EKLQPLIGDYAETKIRKQAITFYYLRLLFSVADDVTPFVWINSLSLVANSEKSLYIQQRNEILKFIKSRETFFKFFANDS
IPNELFDTPDSVVLFLDIHYCISPNSSIEINANDSLLVSYVKWVEYLHLPKNLHSKFTSFAKDIIANTSNTVDQSQYSLL
CTIFENWDATLEKCQWLDYKEVSSTQQLDKLLLELTSTDEINEDNMWFISSKLMKLVMSLKTVLKVTEYKSSVSDSDFME
FANRVISAKIPDRNYLHYKQTALAACHEYFTFVLENGSLEKTVKYINFLTTHFESTDYLCRLQITRSLIKLLSSIHSYYN
DSNVAEGTKNMISTLWASQTVDRLVAVERKLHVAYFDLILSPEILDLSKDEDLAEALESVLEEAIEYSFARRGILPLITS
KILKYFVQAKSDSKTDSRYRVPLWMGRIMTKIYTYRQSSMNLFKLEPAIASMVDAHPLFFGQTSLFRTEYRFQDVDSKIH
CILFFASLEYKSRDSLFCKDLFYYILGKDQIGGGDGINNNTPYRIFVPKKVNDGLETAQRILALQILLVLSKAFTEGTST
VSKDNIESVKSLFDEFIPLLDSEPSPVARIYIEWILASFNLFLLKNKLICTETDPELLKKLALDVAQNDHIINEMEHSEA
TPRIIGSLERIGLLTARALKDAENPFIPFFYYREYIQRLIPFSTSNKATIRHGSVAMLVSLDEEFEQLQYSSDKNGFVNN
GTFIVTKDIKELITNICNHARGFETFNQYRSGSNSIWDINKDFTLLGIFGGVTQRLSDHEVAIITLDDFKNCSSYENIDD
VGSNWLVKIPFEVPTGFDVNYKTWKPATSESTEEESEKVTTIVSAADKKSKPEQDESSSTTVAAPLQTKSGFWNAVLDGT
LGEEGRDADRVSRGDLIVVASLVDKAPNLGGICRLSEVLGAKLLCLGDLAVKKSTAFKSVAVTADRWMPMVEVKPNDVAE
YLQERKKLGYTLIGLEQTDKSVKLDQHLNFPKKSVILLGKEREGIPGPLLAELDFCVEIQQTGVIRSMNIQTATAIIVNA
YSVQHNV

GO term prediction

Biological Process

GO:0006396 RNA processing

Molecular Function

GO:0003723 RNA binding
GO:0005488 binding
GO:0008173 RNA methyltransferase activity

Cellular Component