Protein

MCA_03352_1

Length
839 amino acids

Gene name: HCA4

Description: ATP-dependent RNA helicase DBP4

Browser: contigB:4130478-4133098+

RNA-seq: read pairs 659, FPKM 9.7, percentile rank 25.4% (100% = highest expression)

Protein function

Annotation:HCA4ATP-dependent RNA helicase DBP4
KEGG:K14776DDX10 ATP-dependent RNA helicase DDX10/DBP4 [EC:3.6.4.13]
EGGNOG:0PFU2DBP4ATP-dependent RNA helicase required for ribosome biogenesis. Involved in the release of U14 snoRNA in pre-ribosomal complexes. Required for pre-rRNA cleavage at site A2 (By similarity)
SGD closest match:S000003570HCA4ATP-dependent RNA helicase HCA4
CGD closest match:CAL0000199884DBP4ATP-dependent RNA helicase DBP4

Protein alignments

%idAln lengthE-value
MIA_01625_168.13%6590.0MIA_01625_1
A0A0J9XDM2_GEOCN57.06%6940.0RNA helicase OS=Geotrichum candidum GN=BN980_GECA11s03893g PE=3 SV=1
A0A167EBP8_9ASCO55.02%6870.0RNA helicase OS=Sugiyamaella lignohabitans GN=HCA4 PE=3 SV=1
DBP4_YARLI54.47%6940.0ATP-dependent RNA helicase DBP4 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=DBP4 PE=3 SV=1
A0A060T8Z2_BLAAD54.24%6840.0RNA helicase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D06974g PE=3 SV=1
A0A1E3PTN5_9ASCO56.11%6630.0RNA helicase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_68484 PE=3 SV=1
DBP4_CANAL51.08%6970.0ATP-dependent RNA helicase DBP4 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=DBP4 PE=3 SV=1
DBP4_YEAST52.78%6840.0ATP-dependent RNA helicase HCA4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HCA4 PE=1 SV=2
UniRef50_P2044852.78%6840.0ATP-dependent RNA helicase HCA4 n=108 Tax=Fungi TaxID=4751 RepID=DBP4_YEAST
A0A1E4TKS4_9ASCO49.93%7050.0RNA helicase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_85610 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.7182

Protein family membership

None predicted.

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 600 700 839

Detailed signature matches

  1. Domain IPR014014
    RNA helicase, DEAD-box type, Q motif
    1. PS51195 (Q_MOTIF)
  2. Domain IPR014001
    Helicase superfamily 1/2, ATP-binding domain
    1. PS51192 (HELICASE_A...)
    2. SM00487 (ultradead3)
  3. Domain IPR011545
    DEAD/DEAH box helicase domain
    1. PF00270 (DEAD)
  4. Domain IPR027417
    P-loop containing nucleoside triphosphate hydrolase
    1. SSF52540 (P-loop co...)
  5. Domain IPR001650
    Helicase, C-terminal
    1. PS51194 (HELICASE_CTER)
    2. SM00490 (helicmild6)
    3. PF00271 (Helicase_C)
  6. Domain IPR025313
    Domain of unknown function DUF4217
    1. SM01178 (DUF4217_3)
    2. PF13959 (DUF4217)
  7. SITE IPR000629
    ATP-dependent RNA helicase DEAD-box, conserved site
    1. PS00039 (DEAD_ATP_H...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:3.40.50...
  3. cd00079 (HELICc)
  4. cd00268 (DEADc)
  5. mobidb-lite (disord...)

Residue annotation

  1. ATP binding site c...
  2. Mg++ binding site ...
  3. motif III cd00268
  4. nucleotide binding...
  5. ATP-binding site c...

Protein sequence

>MCA_03352_1
MAKKIGKKKTGDLRKILKTKEEEEFRQLIERVEQFDLENTNITKFKDLPLSKETQKGLDKSHYVELTDIQKRAIAPALKD
KDILGSAPTGSGKTLAFLIPLVEKLYRLRWNREDGLGALIISPTRELALQIFKELKKVGRCHELSAGLIIGGKDVQTEAE
RLDAINILIATPGRLLQHMDQTPNFIVDNLQVLVLDEADRILDAGFKKTLDAILENLPKDRQTMLYSATQTQSVSDLARL
SLTRSANKKPEYITTTGSSTATDSNEKATVVPEKLEQYYIITTIPDKISVLYSFLKTHLKSKIIVFFASSKQVRFIYETF
RRLQPGIPLIQLHGKQRQQARIDATTKFSKSSNACLFATDIVARGLDFPTVDWVVQVDAPDDVSTYIHRVGRTARSNQAG
KSLIFITPKEEEGGLVKKIELRVKREIEKLNVKESKRKSIQDKLQAMCFEDAELKYLGQRAFICYVRSIYLQHDKEVFNI
NDIPMEEFATSMGLPGAPKIKLAMTSEAKIKMKKNMPHALIKLNKADKEGGEEEDDENKVRTRYDRMFERKNQNVLSEHY
MKMAGINKDELEDDDEDDDFMKIKRKDHEIKDDDSEKENQSESESEGEKEENDDDTLKPVLHDVPTSKRAMKRALSKKQR
VLTSSKVGTKIKFDDEGNAHSIYELEDLEDFKKQGDSKKQIEEFISKEKKEMEVIDREDKEIDKTKRIEKKRRRKEFERL
AMEREYGVEGGYDDDDEFEQEEEEAEERNGGNYEDDDENEDIEQQRLNRRERRRRERKEMGLEDKGLFGAEDEDEYRNVI
EDPEEKRQTKRRKKDRIIEVEDPDNLKDLEALSRELLSR

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003676 nucleic acid binding
GO:0005524 ATP binding

Cellular Component

None predicted.