MCA_03231_1

Protein

Length

1,203 amino acids

RNA-seq: read pairs 1841, FPKM 18.9, percentile rank 40.0% (100% = highest expression)

Protein function

EGGNOG:	0PGBQ	FG00841.1	rna-binding protein
SGD closest match:	S000002982	PUF4	Pumilio homology domain family member 4
CGD closest match:	CAL0000177215	orf19.5381	Uncharacterized protein

Protein alignments

	%id	Aln length	E-value
MIA_01628_1	60.00%	360	7e-135	MIA_01628_1
UniRef50_A0A1E3PM02	59.30%	344	3e-130	Uncharacterized protein n=1 Tax=Nadsonia fulvescens var. elongata DSM 6958 TaxID=857566 RepID=A0A1E3PM02_9ASCO
A0A161HH34_9ASCO	61.18%	322	7e-132	Puf4p OS=Sugiyamaella lignohabitans GN=PUF4 PE=4 SV=1
A0A1E3PIP6_9ASCO	58.54%	328	2e-134	ARM repeat-containing protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_36562 PE=4 SV=1
A0A060T4L6_BLAAD	58.15%	325	7e-128	ARAD1C41778p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C41778g PE=4 SV=1
A0A1E4TJ99_9ASCO	54.33%	335	5e-125	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_22238 PE=4 SV=1
Q6C6D4_YARLI	51.78%	309	1e-99	YALI0E10395p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E10395g PE=4 SV=1
PUF4_YEAST	44.57%	341	1e-81	Pumilio homology domain family member 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PUF4 PE=1 SV=2
A0A1D8PJ46_CANAL	41.06%	358	1e-72	Uncharacterized protein OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.5381 PE=4 SV=1
A0A0J9YHB9_GEOCN	29.89%	348	2e-49	Similar to Saccharomyces cerevisiae YGL178W MPT5 Member of the Puf family of RNA-binding proteins OS=Geotrichum candidum GN=BN980_GECA01s02122g PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0978

Protein family membership

None predicted.

Domains and repeats

Domain
408 - 531
Myc-type, basic helix-loop-helix (bHLH) domain (IPR011598)
708 - 1,051
Armadillo-type fold (IPR016024)
Armadillo-like helical (IPR011989)
Pumilio homology domain (IPR033133)
Pumilio, RNA binding domain (IPR033712)
Repeat
730 - 765
Pumilio RNA-binding repeat (IPR001313)
766 - 801
Pumilio RNA-binding repeat (IPR001313)
802 - 838
Pumilio RNA-binding repeat (IPR001313)
839 - 874
Pumilio RNA-binding repeat (IPR001313)
875 - 910
Pumilio RNA-binding repeat (IPR001313)
911 - 946
Pumilio RNA-binding repeat (IPR001313)
947 - 982
Pumilio RNA-binding repeat (IPR001313)
983 - 1,020
Pumilio RNA-binding repeat (IPR001313)

1 200 400 600 800 1000 1203

Detailed signature matches

IPR011598

Myc-type, basic helix-loop-helix (bHLH) domain
1. G3DSA:4.10.28...
  
  408 - 531
  
  GENE3D
  G3DSA:4.10.280.10 (G3DSA:4.10.280.10)
IPR016024

Armadillo-type fold
1. SSF48371 (ARM repeat)
  
  716 - 1,043
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
IPR011989

Armadillo-like helical
1. G3DSA:1.25.10.10
  
  710 - 1,051
  
  GENE3D
  G3DSA:1.25.10.10 (G3DSA:1.25.10.10)
IPR033133

Pumilio homology domain
1. PS50303 (PUM_HD)
  
  708 - 1,046
  
  PROSITE profiles
  PS50303 (PUM_HD)
IPR033712

Pumilio, RNA binding domain
1. cd07920 (Pumilio)
  
  729 - 1,042
  
  CDD
  cd07920 (Pumilio)
IPR001313

Pumilio RNA-binding repeat
1. PS50302 (PUM)
  
  875 - 910
  
  PROSITE profiles
  PS50302 (PUM)
  
  730 - 765
  
  PROSITE profiles
  PS50302 (PUM)
  
  802 - 837
  
  PROSITE profiles
  PS50302 (PUM)
  
  911 - 946
  
  PROSITE profiles
  PS50302 (PUM)
  
  839 - 874
  
  PROSITE profiles
  PS50302 (PUM)
  
  947 - 982
  
  PROSITE profiles
  PS50302 (PUM)
  
  766 - 801
  
  PROSITE profiles
  PS50302 (PUM)
  
  983 - 1,020
  
  PROSITE profiles
  PS50302 (PUM)
2. PF00806 (PUF)
  
  841 - 872
  
  Pfam
  PF00806 (PUF)
  
  804 - 835
  
  Pfam
  PF00806 (PUF)
  
  877 - 909
  
  Pfam
  PF00806 (PUF)
  
  948 - 980
  
  Pfam
  PF00806 (PUF)
  
  987 - 1,016
  
  Pfam
  PF00806 (PUF)
  
  732 - 753
  
  Pfam
  PF00806 (PUF)
  
  911 - 939
  
  Pfam
  PF00806 (PUF)
  
  769 - 797
  
  Pfam
  PF00806 (PUF)
3. SM00025 (pum_5)
  
  839 - 874
  
  SMART
  SM00025 (pum_5)
  
  985 - 1,019
  
  SMART
  SM00025 (pum_5)
  
  766 - 801
  
  SMART
  SM00025 (pum_5)
  
  802 - 838
  
  SMART
  SM00025 (pum_5)
  
  875 - 910
  
  SMART
  SM00025 (pum_5)
  
  947 - 982
  
  SMART
  SM00025 (pum_5)
  
  911 - 946
  
  SMART
  SM00025 (pum_5)
  
  730 - 765
  
  SMART
  SM00025 (pum_5)

no IPR

Unintegrated signatures

mobidb-lite (disord...)

1,051 - 1,162

MobiDB Lite
mobidb-lite (disorder_prediction)

378 - 410

MobiDB Lite
mobidb-lite (disorder_prediction)

264 - 340

MobiDB Lite
mobidb-lite (disorder_prediction)

608 - 724

MobiDB Lite
mobidb-lite (disorder_prediction)

429 - 528

MobiDB Lite
mobidb-lite (disorder_prediction)

1,179 - 1,203

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

RNA binding site c...

781N

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

782Y

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

785Q

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

817T

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

818R

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

821Q

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

855H

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

858Q

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

890C

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

891C

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

894Q

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

923P

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

924Y

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

926N

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

927Y

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

930Q

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

959K

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

960Y

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

962S

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

963N

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

966E

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

997S

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

998Y

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

1,000N

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

1,001Y

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

1,004Q

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

1,034Y

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

1,037R

RNA binding site
781N, 782Y, 785Q, 817T, 818R, 821Q, 855H, 858Q, 890C, 891C, 894Q, 923P, 924Y, 926N, 927Y, 930Q, 959K, 960Y, 962S, 963N, 966E, 997S, 998Y, 1,000N, 1,001Y, 1,004Q, 1,034Y, 1,037R
CDD
cd07920 (Pumilio)

Protein sequence

>MCA_03231_1
MNNHSLSSSSSSSSGVVAATTTTILASNNNYQQHSSNESSLNDYLSSLDNHNQQQKKRLSHHGHSYSMFVPSSSSYSFLN
STSQSTTAAAVISSSSTNTNTKMSPTINPPYISSPPLSSSTHSLSFMAPNNTNNTSNTNNNIQFHKAKSTSISFIPESQL
SEMNSFINQQKQPSSSLTMPLHSRSLSTSSYNFFYDNNNNNNNNNISLTSSFNNDNLLSSNLVGLNGNVGGGGGNNNNIG
AATGIATNSNINNLDSITATSTSSSISTFSTSSPGLNKSPIPSNNSIEYMNFSFPNNNQSTNNLDNNNNNNNNNNNQSPL
LVSNNSQQNNNNIASPNNFNFAFPNNSTSINKSISHDTYLQRETNNEQFPILVRRESFQQQQQSINENSITSPSLPYESN
FRPSYTPQYHQKRRSIGAFTTLSSQNTLNSSLLSNDNDNNNNNNNNNNVDYVPELTRDLPSSRGHHRYSQSLSISNIHSQ
PKIPSRSHHRPTTSLSGNIDFFSQQQQQQQQQYQHQQQRSTNQQSSFYQQQQINDNALSFDLPTPTASSIQRTNLDAFNM
NLLNKSLDQSIVQNKLLQQQQQQQQQQQQKVRPLSLDENYFVRNNYSYNNNNQQLQNNNSTNDVNSLIISSNNIPQKPPT
PSDSYFLSSNHLDSSSSVSLSNSSNIPMESIKLDRSESTSSSSSTSNPISSTNTNNNSNNNNNNNRRRYSTTRRHQQESS
NKYTDAKIEQFVNDIYSLCKDQHGCRFLQKTLEDKNPAHLEMIFKEVAPHMSELMTDPFGNYLCQKLLEHSNTQQRTELV
RNSSKNLIEIALDPHGTRALQKMIEFAKAPEQIHIITNALHDKVVDLIKDLNGNHVIQKCLNGLSFEHTQFIIDAVSSNC
VVVGTHRHGCCVLQRCIDHSSQAQKNQLISEIINNSCELVQDPYGNYVAQYVLDLRKSQYSEPLIKTFLGQVCMLSQQKY
SSNVMEKCLTCAEPSTRRLLIDELINSNELTTMVRDSYANYVIQKALDYADEEKKKIVASKLASIMPSIRTTPYGKRLES
KLQMYLTSPNGSGVGTPISSSSSSSLSSSSNNNSNNNNNNSAISRSSSLRHGNNNNNNKPPVGQPIMNNPQMMIPGGGGP
YIPMGISHNQFHPHHHHQGYHHHHQQIPFQGPIPPQPSHNQQIVQQMIQQNQMVGGYFDFQQNLPNNNNNRNNNRSYQGQ
QKQ

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003723 RNA binding
GO:0005488 binding
GO:0046983 protein dimerization activity

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

MCA_03231_1

Protein function

Protein alignments

Mitochondrial localization by mitoprotII

Protein family membership

Domains and repeats

Detailed signature matches

Residue annotation

Protein sequence

GO term prediction

Biological Process

Molecular Function

Cellular Component