Protein
MCA_02691_1
Length
328 amino acids
Gene name: PNG1
Description: Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
Browser: contigB:2068522-2069630-
RNA-seq: read pairs 1124, FPKM 42.2, percentile rank 61.5% (100% = highest expression)
Protein function
| Annotation: | PNG1 | Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase | |
|---|---|---|---|
| KEGG: | K01456 | E3.5.1.52 | peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase [EC:3.5.1.52] |
| EGGNOG: | 0PJHM | PNG1 | Specifically deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins |
| SGD closest match: | S000006017 | PNG1 | Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase |
| CGD closest match: | CAL0000182135 | PNG1 | Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase |
Protein alignments
| %id | Aln length | E-value | ||
|---|---|---|---|---|
| MIA_05803_1 | 61.96% | 326 | 2e-136 | MIA_05803_1 |
| A0A0J9XB89_GEOCN | 58.31% | 307 | 8e-112 | Similar to Saccharomyces cerevisiae YPL096W PNG1 Conserved peptide N-glycanase required for deglycosylation of misfolded glycoproteins during proteasome-dependent degradation OS=Geotrichum candidum GN=BN980_GECA07s05136g PE=4 SV=1 |
| A0A1E3PPN6_9ASCO | 54.05% | 309 | 8e-104 | Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_81884 PE=4 SV=1 |
| A0A060T3G3_BLAAD | 50.97% | 308 | 5e-96 | ARAD1C37180p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C37180g PE=4 SV=1 |
| UniRef50_A0A060T3G3 | 50.97% | 308 | 1e-92 | ARAD1C37180p n=1 Tax=Blastobotrys adeninivorans TaxID=409370 RepID=A0A060T3G3_BLAAD |
| A0A1E4TJ07_9ASCO | 46.28% | 309 | 1e-82 | Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_11339 PE=4 SV=1 |
| PNG1_YARLI | 44.48% | 299 | 4e-82 | Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=PNG1 PE=3 SV=1 |
| PNG1_YEAST | 42.97% | 263 | 1e-58 | Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PNG1 PE=1 SV=1 |
| PNG1_CANAL | 36.24% | 287 | 6e-50 | Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PNG1 PE=3 SV=1 |
Mitochondrial localization by mitoprotII
Probability of mitochondrial location: 0.1838
Protein family membership
None predicted.
Domains and repeats
-
Domain
1
50
100
150
200
250
300
328
Detailed signature matches
no IPR
Unintegrated signatures
-
-
-
SSF54001 (Cysteine ...)
-
mobidb-lite (disord...)
Protein sequence
>MCA_02691_1 MAFDYEGFIHEFATSFYLKSTNKNDFTKQLVALSETPMAYEDNDLLDYALTFLPLEDFYKKAEEKYAKDKTWSEQDYALQ ECVYWFKHSFFLWVNNPLCDICGGDTNPIGMGTPTHQEQQDGASRVELYKCVRCSAIVRFPRYNNLRTLLRTRKGRCGEF SNCFTLLCRAMGARARWVWNSEDHVWTETYSDKQKRWVHFDPSEGIYDKPLVYQDGWGKKMSYVIAFSCEGAFDVTNRYV RKPEMALPRTKCTEKQLRLALAGIKQKRRDHLNLSKKELEELQREDAAEKKELESYVSGPSKTDSSVGPRESGAGEWTKS RGEDGSKK
GO term prediction
Biological Process
None predicted.
Molecular Function
None predicted.
Cellular Component
None predicted.