Protein

MCA_02527_1

Length
855 amino acids

Gene name: EFT1

Description: Elongation factor 2

Browser: contigB:1563747-1566757+

RNA-seq: read pairs 105725, FPKM 1526.1, percentile rank 97.4% (100% = highest expression)

Protein function

Annotation:EFT1Elongation factor 2
KEGG:K03234EEF2 elongation factor 2
EGGNOG:0PF9TEFT1elongation factor 2
SGD closest match:S000005659EFT1Elongation factor 2
CGD closest match:CAL0000189676EFT2Elongation factor 2

Protein alignments

%idAln lengthE-value
EF2_YEAST88.05%8370.0Elongation factor 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=EFT1 PE=1 SV=1
UniRef50_P3232488.05%8370.0Elongation factor 2 n=1141 Tax=Eukaryota TaxID=2759 RepID=EF2_YEAST
A0A161HIJ9_9ASCO88.92%8300.0Elongation factor 2 OS=Sugiyamaella lignohabitans GN=EFT1 PE=4 SV=1
A0A060SWD6_BLAAD86.74%8370.0ARAD1A03520p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A03520g PE=4 SV=1
A0A1E4TLM5_9ASCO85.19%8370.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_90500 PE=4 SV=1
EF2_CANAL85.54%8370.0Elongation factor 2 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=EFT2 PE=1 SV=2
Q6CI99_YARLI85.42%8370.0YALI0A00352p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_A00352g PE=4 SV=1
MIA_03300_182.89%8300.0MIA_03300_1
A0A1E3PJD5_9ASCO79.81%8370.0p-loop containing nucleoside triphosphate hydrolase protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_46233 PE=4 SV=1
A0A0F7RR50_GEOCN90.64%2671e-173Similar to Saccharomyces cerevisiae YOR133W EFT1 Elongation factor 2 (EF-2). Partial (No 3' end) (Partial) (Fragment) OS=Geotrichum candidum GN=BN980_GECA14s01390g PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.2287
Predicted cleavage: 35

Protein family membership

None predicted.

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 600 700 800 855

Detailed signature matches

  1. Domain IPR027417
    P-loop containing nucleoside triphosphate hydrolase
    1. SSF52540 (P-loop co...)
  2. Domain IPR005225
    Small GTP-binding protein domain
    1. TIGR00231
  3. Domain IPR000795
    Transcription factor, GTP-binding domain
    1. PS51722 (G_TR_2)
    2. PF00009 (GTP_EFTU)
    3. PR00315 (ELONGATNFCT)
  4. Domain IPR009000
    Translation protein, beta-barrel domain
    1. SSF50447 (Translati...)
  5. Domain IPR004161
    Translation elongation factor EFTu-like, domain 2
    1. PF03144 (GTP_EFTU_D2)
  6. Domain IPR035647
    EF-G domain III/V-like
    1. SSF54980 (EF-G C-te...)
  7. Domain IPR020568
    Ribosomal protein S5 domain 2-type fold
    1. SSF54211 (Ribosomal...)
  8. Domain IPR014721
    Ribosomal protein S5 domain 2-type fold, subgroup
    1. G3DSA:3.30.23...
  9. Domain IPR005517
    Translation elongation factor EFG/EF2, domain IV
    1. PF03764 (EFG_IV)
    2. SM00889 (EFG_IV_2)
  10. Domain IPR000640
    Elongation factor EFG, domain V-like
    1. PF00679 (EFG_C)
    2. SM00838 (EFG_C_a)
  11. SITE IPR031157
    Tr-type G domain, conserved site
    1. PS00301 (G_TR_1)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:2.40.30.10
  2. G3DSA:3.30.70...
  3. G3DSA:3.30.70...
  4. G3DSA:3.40.50...
  5. G3DSA:3.90.14...
  6. PF14492 (EFG_II)
  7. cd01681 (aeEF2_snRN...)
  8. cd01885 (EF2)
  9. cd03700 (EF2_snRNP_...)
  10. cd04096 (eEF2_snRNP...)
  11. cd16261 (EF2_snRNP_III)

Residue annotation

  1. G1 box cd01885
  2. putative GEF inter...
  3. GTP/Mg2+ binding s...
  4. Switch I region cd...
  5. G2 box cd01885
  6. G3 box cd01885
  7. Switch II region c...
  8. G4 box cd01885
  9. G5 box cd01885

Protein sequence

>MCA_02527_1
MGMNVVVSFSKDQYLYAVLSQIRALMDRVPNVRNMSVIAHVDHGKSTLTDSLVQRAGIISAAKAGEARFTDTRKDEQERG
ITIKSTAISLYSEMSDEDCKEIKQKTDGNQFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDAIEGVCVQTETVLRQAL
GERIKPVLVVNKVDRALLELQVTKEDLYQSFARTVESVNVIIATYFDKSLGDVQVYPEKGTVAFASGLHGWAFTVRQFAI
RYSKKFGVDREKMMTRLWGDSFFNPKTKKWTNKDKDADGKPLERAFNVFILDPIFRIFDSIMNFKKTEIPTLLDKLGIVL
KGDEKDLEGKALLKVVMRKFLPAADALLEMIVIHLPSPVTAQAYRAETLYEGPSDDASCISIKNCDPKADLMLYVSKMVP
TSDKGRFYAFGRVFAGTVRSGMKVRIQGPNYIPGKKDDLFIKAIQRTVLMMGRTVEPIDDCPAGNIVGLVGVDQFLLKSG
TLTTSEHAHNLKVMKFSVSPVVQVAVDVKNANDLPKLVEGLKRLSKSDPCVLTTISESGEHIVAGSGELHLEICLKDLEE
DHAGIPLKISPPIVSYRETVTAESSMTALSKSPNKHNRIYCKAEPIDEELSLAIESGKINPRDDFKARARIMSDEYGWDV
SDARKIWCFGPEGNGPNLVVDQTKAVQYLSEIKDSVVAAFQWATKEGPMFGENMRSVRVNILDVTLHPDAIHRGGGQIIP
TMRRVTYAALLLAQPAIQEPFFLVEIQCPENAIGGIYSCLNTKRGTVISEEQRPGTPLFTVKAYLPVNESFGFTGDLRQA
TGGQAFPQMVFDHWEAMNSDPLDPSSKAGQIVLAGRKRHGMKEAVPGYEEYYDKL

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003924 GTPase activity
GO:0005525 GTP binding

Cellular Component

None predicted.