Protein

MCA_02098_1

Length
775 amino acids

Gene name: LEU1

Description: 3-isopropylmalate dehydratase

Browser: contigB:236356-238684-

RNA-seq: read pairs 6277, FPKM 99.9, percentile rank 78.6% (100% = highest expression)

Protein function

Annotation:LEU13-isopropylmalate dehydratase
KEGG:K01702LEU1 3-isopropylmalate dehydratase [EC:4.2.1.33]
EGGNOG:0PH7VLEU1Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate (By similarity)
SGD closest match:S000002977LEU13-isopropylmalate dehydratase
CGD closest match:CAL0000187273LEU13-isopropylmalate dehydratase

Protein alignments

%idAln lengthE-value
MIA_05516_184.43%7770.0MIA_05516_1
A0A0J9X404_GEOCN82.09%7760.03-isopropylmalate dehydratase OS=Geotrichum candidum GN=BN980_GECA02s06126g PE=3 SV=1
A0A167C6K9_9ASCO76.08%7610.03-isopropylmalate dehydratase OS=Sugiyamaella lignohabitans GN=LEU1 PE=3 SV=1
A0A060T3Q1_BLAAD74.90%7770.03-isopropylmalate dehydratase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C41580g PE=3 SV=1
Q6CG29_YARLI75.52%7680.03-isopropylmalate dehydratase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_B01364g PE=3 SV=1
A0A1E3PMT5_9ASCO70.01%7770.03-isopropylmalate dehydratase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_23691 PE=3 SV=1
A0A1D8PRP8_CANAL70.05%7780.03-isopropylmalate dehydratase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=LEU1 PE=3 SV=1
UniRef50_A0A0F4XDM469.54%7780.03-isopropylmalate dehydratase n=9 Tax=saccharomyceta TaxID=716545 RepID=A0A0F4XDM4_HANUV
A0A1E4TIS3_9ASCO68.67%7660.03-isopropylmalate dehydratase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_221 PE=3 SV=1
LEUC_YEAST69.72%7530.03-isopropylmalate dehydratase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LEU1 PE=1 SV=3

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.3914

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 600 700 775

Detailed signature matches

  1. Family IPR012235
    3-isopropylmalate dehydratase, fused small/large subunit
    1. PIRSF001418 (ACN)
  2. Family IPR004430
    3-isopropylmalate dehydratase, large subunit
    1. TIGR00170
    2. MF_01026 (LeuC_type1)
  3. Family IPR004431
    3-isopropylmalate dehydratase, small subunit
    1. TIGR00171
    2. MF_01031 (LeuD_type1)
  4. Domain IPR015931
    Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3
    1. G3DSA:3.30.49...
  5. Domain IPR001030
    Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha domain
    1. PR00415 (ACONITASE)
    2. SSF53732 (Aconitase...)
    3. PF00330 (Aconitase)
  6. Domain IPR033941
    3-Isopropylmalate dehydratase, catalytic domain
    1. cd01583 (IPMI)
  7. Domain IPR015932
    Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 2
    1. G3DSA:3.40.10...
  8. Domain IPR015928
    Aconitase/3-isopropylmalate dehydratase, swivel
    1. G3DSA:3.20.19.10
    2. SSF52016 (LeuD/IlvD...)
  9. Domain IPR000573
    Aconitase A/isopropylmalate dehydratase small subunit, swivel domain
    1. PF00694 (Aconitase_C)
  10. Domain IPR033940
    3-isopropylmalate dehydratase, swivel domain
    1. cd01577 (IPMI_Swivel)
  11. SITE IPR018136
    Aconitase family, 4Fe-4S cluster binding site
    1. PS00450 (ACONITASE_1)
    2. PS01244 (ACONITASE_2)

Residue annotation

  1. substrate binding ...
  2. ligand binding sit...
  3. substrate binding ...

Protein sequence

>MCA_02098_1
MPSIDSTPKTLYDKVFEDHIVHADENNTYLIYIDRHLVHEVTSPQAFEGLKNAGRQVRRRDCTLATVDHNIPTSSRANFK
NIATFIEQDDSRLQVMTLEQNVKDFNVRYFGMEDSRQGIVHVVGPEQGFTLPGTTVVCGDSHTSTHGAFGALAFGIGTSE
VEHVLATQTLIQTKSKNMLINVSGKLNPGITSKDLILHIIGHIGTAGGTGMVIEFAGEAIRDLSMEARMSMCNMAIEGGA
RAGMVAPDETTFKYIKGRPLAPQGEEWEKALAYWKTLKSDPGAHFDVVVEIDAANIIPTVTWGTSPQDALPITGKVPDPA
DYSDPIRKAGVERALQYMGLKANTPLQEIQVDKVFIGSCTNSRIEDLRLAASIVKGKKKADNVSRVLVVPGSGLVKTQAE
KEGLDKIFIDAGFEWREAGCSMCLGMNPDILSPHERCASTSNRNFEGRQGALSRTHLVSPSMAAAAAITGHLTDVREFLP
KDSSAPISIEVDNDTDFNAQEKEPMYPDEIEITTDAPVSSAAAAEEIDVAAAKAAPKKGSTGIPVFQIETGIACPIAKAN
VDTDAIIPKQFLKTIKRTGLSSGLFYELRFAKNEKTGEMEKTDFALNVKPWDAAKILVVSGDNFGCGSSREHAPWALADF
GIRCIIAPSFGDIFYNNSFKNGLLLIRAPADLIKEKLIPIANNGGELTVDLPKQQVRSASGEVLVPHFDVDDFRKNCLLN
GLDDIGITLQKESFIKAYEDRRRERFSFLEDSSHGPIAPVKGAKKSIYGVKAQEW

GO term prediction

Biological Process

GO:0008152 metabolic process
GO:0009098 leucine biosynthetic process

Molecular Function

GO:0003861 3-isopropylmalate dehydratase activity
GO:0051539 4 iron, 4 sulfur cluster binding

Cellular Component

GO:0009316 3-isopropylmalate dehydratase complex