MCA_01987_1

Protein

Length

1,470 amino acids

Gene name: INO80

Description: Putative DNA helicase INO80; ATPase and nucleosome spacing factor; subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro

Browser: contigA:6034989-6039469+

RNA-seq: read pairs 2946, FPKM 24.7, percentile rank 47.4% (100% = highest expression)

Protein function

Annotation:		INO80	Putative DNA helicase INO80; ATPase and nucleosome spacing factor; subunit of complex containing actin and actin-related proteins that has chromatin remodeling activity and 3' to 5' DNA helicase activity in vitro
KEGG:	K11665	INO80	DNA helicase INO80 [EC:3.6.4.12]
EGGNOG:	0PFTZ	INO80	DNA helicase component of the INO80 complex which remodels chromatin by shifting nucleosomes and is involved in DNA repair (By similarity)
SGD closest match:	S000003118	INO80	Putative DNA helicase INO80
CGD closest match:	CAL0000196418	INO80	Putative DNA helicase INO80

Protein alignments

	%id	Aln length
MIA_06343_1	75.73%	1063	MIA_06343_1
A0A0J9XH44_GEOCN	67.09%	1091	Similar to Saccharomyces cerevisiae YGL150C INO80 ATPase and nucleosome spacing factor OS=Geotrichum candidum GN=BN980_GECA17s01154g PE=4 SV=1
A0A167CPE9_9ASCO	64.38%	1123	Chromatin-remodeling ATPase INO80 OS=Sugiyamaella lignohabitans GN=INO80 PE=4 SV=1
UniRef50_A0A167CPE9	64.38%	1123	Chromatin-remodeling ATPase INO80 n=3 Tax=Saccharomycetales TaxID=4892 RepID=A0A167CPE9_9ASCO
A0A060T5P2_BLAAD	64.34%	1088	ARAD1B05720p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B05720g PE=4 SV=1
A0A1E3PF31_9ASCO	61.00%	1082	Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_52805 PE=4 SV=1
A0A1E4TI66_9ASCO	58.41%	1070	Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_19857 PE=4 SV=1
Q6C6J7_YARLI	60.43%	1021	YALI0E09012p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E09012g PE=4 SV=2
INO80_YEAST	56.79%	1016	Putative DNA helicase INO80 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=INO80 PE=1 SV=1
INO80_CANAL	55.21%	978	Putative DNA helicase INO80 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=INO80 PE=3 SV=2

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1127

Protein family membership

None predicted.

Domains and repeats

Domain
406 - 537
DBINO domain (IPR020838)
620 - 819
Helicase superfamily 1/2, ATP-binding domain (IPR014001)
1,194 - 1,347
Helicase, C-terminal (IPR001650)
Domain
601 - 853
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
855 - 927
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
1,185 - 1,346
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Domain
637 - 923
SNF2-related, N-terminal domain (IPR000330)

1 200 400 600 800 1000 1200 1470

Detailed signature matches

IPR020838

DBINO domain
1. PF13892 (DBINO)
  
  406 - 536
  
  Pfam
  PF13892 (DBINO)
2. PS51413 (DBINO)
  
  412 - 537
  
  PROSITE profiles
  PS51413 (DBINO)
IPR027417

P-loop containing nucleoside triphosphate hydrolase
1. SSF52540 (P-loop co...)
  
  601 - 853
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  855 - 927
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  1,185 - 1,346
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
IPR014001

Helicase superfamily 1/2, ATP-binding domain
1. PS51192 (HELICASE_A...)
  
  636 - 808
  
  PROSITE profiles
  PS51192 (HELICASE_ATP_BIND_1)
2. SM00487 (ultradead3)
  
  620 - 819
  
  SMART
  SM00487 (ultradead3)
IPR000330

SNF2-related, N-terminal domain
1. PF00176 (SNF2_N)
  
  637 - 923
  
  Pfam
  PF00176 (SNF2_N)
IPR001650

Helicase, C-terminal
1. PS51194 (HELICASE_CTER)
  
  1,197 - 1,347
  
  PROSITE profiles
  PS51194 (HELICASE_CTER)
2. SM00490 (helicmild6)
  
  1,223 - 1,306
  
  SMART
  SM00490 (helicmild6)
3. PF00271 (Helicase_C)
  
  1,194 - 1,306
  
  Pfam
  PF00271 (Helicase_C)

no IPR

Unintegrated signatures

Coil

207 - 245

COILS
Coil (Coil)

476 - 520

COILS
Coil (Coil)
G3DSA:3.40.50...

591 - 852

GENE3D
G3DSA:3.40.50.10810 (G3DSA:3.40.50.10810)
G3DSA:3.40.50...

1,180 - 1,344

GENE3D
G3DSA:3.40.50.300 (G3DSA:3.40.50.300)
cd00046 (DEXDc)

644 - 790

CDD
cd00046 (DEXDc)
cd00079 (HELICc)

1,189 - 1,314

CDD
cd00079 (HELICc)
mobidb-lite (disord...)

202 - 394

MobiDB Lite
mobidb-lite (disorder_prediction)

59 - 80

MobiDB Lite
mobidb-lite (disorder_prediction)

1,379 - 1,470

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

ATP binding site c...

652G

ATP binding site
652G, 653L, 654G, 655K, 656T
CDD
cd00046 (DEXDc)

653L

ATP binding site
652G, 653L, 654G, 655K, 656T
CDD
cd00046 (DEXDc)

654G

ATP binding site
652G, 653L, 654G, 655K, 656T
CDD
cd00046 (DEXDc)

655K

ATP binding site
652G, 653L, 654G, 655K, 656T
CDD
cd00046 (DEXDc)

656T

ATP binding site
652G, 653L, 654G, 655K, 656T
CDD
cd00046 (DEXDc)
putative Mg++ bind...

759D

putative Mg++ binding site
759D, 760E, 761A, 762Q
CDD
cd00046 (DEXDc)

760E

putative Mg++ binding site
759D, 760E, 761A, 762Q
CDD
cd00046 (DEXDc)

761A

putative Mg++ binding site
759D, 760E, 761A, 762Q
CDD
cd00046 (DEXDc)

762Q

putative Mg++ binding site
759D, 760E, 761A, 762Q
CDD
cd00046 (DEXDc)
nucleotide binding...

1,217Q

nucleotide binding region
1,217Q, 1,218M, 1,219T, 1,220K, 1,240D, 1,241G, 1,268T, 1,269R, 1,270A
CDD
cd00079 (HELICc)

1,218M

nucleotide binding region
1,217Q, 1,218M, 1,219T, 1,220K, 1,240D, 1,241G, 1,268T, 1,269R, 1,270A
CDD
cd00079 (HELICc)

1,219T

nucleotide binding region
1,217Q, 1,218M, 1,219T, 1,220K, 1,240D, 1,241G, 1,268T, 1,269R, 1,270A
CDD
cd00079 (HELICc)

1,220K

nucleotide binding region
1,217Q, 1,218M, 1,219T, 1,220K, 1,240D, 1,241G, 1,268T, 1,269R, 1,270A
CDD
cd00079 (HELICc)

1,240D

nucleotide binding region
1,217Q, 1,218M, 1,219T, 1,220K, 1,240D, 1,241G, 1,268T, 1,269R, 1,270A
CDD
cd00079 (HELICc)

1,241G

nucleotide binding region
1,217Q, 1,218M, 1,219T, 1,220K, 1,240D, 1,241G, 1,268T, 1,269R, 1,270A
CDD
cd00079 (HELICc)

1,268T

nucleotide binding region
1,217Q, 1,218M, 1,219T, 1,220K, 1,240D, 1,241G, 1,268T, 1,269R, 1,270A
CDD
cd00079 (HELICc)

1,269R

nucleotide binding region
1,217Q, 1,218M, 1,219T, 1,220K, 1,240D, 1,241G, 1,268T, 1,269R, 1,270A
CDD
cd00079 (HELICc)

1,270A

nucleotide binding region
1,217Q, 1,218M, 1,219T, 1,220K, 1,240D, 1,241G, 1,268T, 1,269R, 1,270A
CDD
cd00079 (HELICc)
ATP-binding site c...

1,276N

ATP-binding site
1,276N, 1,297Q, 1,301R, 1,304R
CDD
cd00079 (HELICc)

1,297Q

ATP-binding site
1,276N, 1,297Q, 1,301R, 1,304R
CDD
cd00079 (HELICc)

1,301R

ATP-binding site
1,276N, 1,297Q, 1,301R, 1,304R
CDD
cd00079 (HELICc)

1,304R

ATP-binding site
1,276N, 1,297Q, 1,301R, 1,304R
CDD
cd00079 (HELICc)

Protein sequence

>MCA_01987_1
MEVPSRRRGGDAMAISTLLSDSAKSNEEPGQRSVQNSDNNLVLDDAQHANQQQLNILDSSPISSDRHINDASNGIGQHLN
KEQVLASKRRKRGSYLPLAPDNEDLVYDALNPTHMEASYQYSNQLAFQDEKAEYLATLRQRVTNIQIMDERHAINSRDAL
QKKYFTLYSTKADALRKFTAKELCPQAMRNVMRFEELVEKESKRLHRNQLKKERRAQKSAALRAERAEAKAKAEADNEFD
NAEEEDAIHGKKGQKKGAEAKVKSKENESEPTLIKQKRGKIRKSDADKPKANSQIEDDAKASSNTANKQKSRSKSKAPSA
GSKSDSSSRSKLVLLAPAAPLSETSKDSPDEEDEEEDESKKDEETTKNGSSKSLSGPTTGQKSAVPPPPPKLPSQKEIRA
IARLYSNTYESVWKDMARRDANKVHRLVQSSTNTRISNLRKTAVLASKEARRWQFKTNKTIKDLQMKAKRGMREMLAFWK
RNEREERDLRKKAEKEALELAKKEEEMRESRRQARKLNFLITQTELYTHFIGKKLGSVEDENPTEVKDVKDLDFDSDNED
EIHKVALANARSAFEDTQAYAKSFNGESGEIKNVNIDNDEMNFQNPTSLGDIQIPQPKLLSCQLKEYQLKGLSWLANLYE
QGINGILADEMGLGKTVQSISVMAYLAETHNIWGPFLVISPSSTLHNWEQEIAKFVPDFKVLPYWGSTKDRKILRKFWDH
KNVKYTKDSEFHVLITSYQLVVQDASYFQKMKWQYMILDEAQAIKSSSSARWKSLLSFQCRNRLLLTGTPIQNSMQELWA
LLHFIMPTLFDSHDEFSEWFSKDIESHAQSNTQLNEQQLNRLHMILKPFMLRRLKKHVQQELGDKIEIDVYCDLTNRQRE
LYRMLRSQISVMELIEKASSDEGTQSLMNLVMQFRKVCNHPDLFERADVSAAYSFSTPKNTFGPIRESKDTEILYNCENL
INYCIPKLIYRDGGIFNVPSENNFNFGRVKLYLENLFSIWDPSFISETEDMNFLRLLNMSSSEIVKSAKKGLFERAIDSY
HNLASSDSFGELDNDSACHISRMLQLSKPHSLKGASNATGLLANLLNIRQKVLDESIYNFIDPCYNDRVQAPPITLVGSD
RSIPIDQEAILFDHRARSALYPMSLDEEYDYISKGVSIEELPPSNLMPSPCNPRMGFTTIQMPSMNKFVTDSGKLAKLDQ
MLPELKAEDHRVLIYFQMTKMMDLMEEYLTFRQYKYVRLDGSSKLTDRRDLVNDWQTKPELFIFLLSTRAGGLGINLTAA
DTVIFYDSDWNPTIDSQAMDRAHRMGQTRQVTVYRLLVKGTIEERMRDRAKQKMHVQQVVMEGGSGKSGNSVDFQKPGRE
VAYWLLDDDATADALIKKTEEDGESGNGRKPSMKRSSQSMDEESTTGPLPKKPRVKEIEDMYHEGEGEFKDSNNGSPESV
VPSSKSKNSKKGHKKMTTEQRLRAVGSGEF

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003677 DNA binding
GO:0005524 ATP binding

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

MCA_01987_1

Protein function

Protein alignments

Mitochondrial localization by mitoprotII

Protein family membership

Domains and repeats

Detailed signature matches

Residue annotation

Protein sequence

GO term prediction

Biological Process

Molecular Function

Cellular Component