Protein

MCA_01103_1

Length
752 amino acids

Browser: contigA:3507031-3509428+

RNA-seq: read pairs 6843, FPKM 112.3, percentile rank 80.6% (100% = highest expression)

Protein function

KEGG:K01874MARS methionyl-tRNA synthetase [EC:6.1.1.10]
EGGNOG:0PG5DFG08614.1methionyl-tRNA synthetase
SGD closest match:S000003496MES1Methionine--tRNA ligase, cytoplasmic
CGD closest match:CAL0000178172MES1Methionine--tRNA ligase

Protein alignments

%idAln lengthE-value
MIA_00879_173.98%7570.0MIA_00879_1
A0A0J9X8F0_GEOCN70.74%7450.0Similar to Saccharomyces cerevisiae YGR264C MES1 Methionyl-tRNA synthetase OS=Geotrichum candidum GN=BN980_GECA04s03937g PE=3 SV=1
A0A1E3PNK6_9ASCO61.47%7500.0Methionyl-tRNA synthetase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_81632 PE=3 SV=1
A0A167FY98_9ASCO59.14%7710.0Methionine--tRNA ligase MES1 OS=Sugiyamaella lignohabitans GN=MES1 PE=3 SV=1
A0A060TCJ7_BLAAD58.71%7750.0ARAD1D39688p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D39688g PE=3 SV=1
Q6BZY8_YARLI59.60%7500.0YALI0F29843p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F29843g PE=3 SV=1
A0A1D8PP21_CANAL60.40%7450.0Methionine--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=MES1 PE=3 SV=1
A0A1E4TFJ2_9ASCO58.01%7550.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_106703 PE=3 SV=1
SYMC_YEAST56.68%7480.0Methionine--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MES1 PE=1 SV=4
UniRef50_P0095856.68%7480.0Methionine--tRNA ligase, cytoplasmic n=102 Tax=Fungi TaxID=4751 RepID=SYMC_YEAST

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1735

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 600 700 752

Detailed signature matches

  1. Family IPR014758
    Methionyl-tRNA synthetase
    1. TIGR00398
  2. Family IPR023458
    Methionine-tRNA ligase, type 1
    1. MF_00098 (Met_tRNA_...)
  3. Domain IPR014729
    Rossmann-like alpha/beta/alpha sandwich fold
    1. G3DSA:3.40.50...
  4. Domain IPR015413
    Methionyl/Leucyl tRNA synthetase
    1. PF09334 (tRNA-synt_1g)
  5. Domain IPR033911
    Methioninyl-tRNA synthetase core domain
    1. PR01041 (TRNASYNTHMET)
    2. cd00814 (MetRS_core)
  6. Domain IPR029038
    Methionyl-tRNA synthetase, Zn-domain
    1. SSF57770 (Methionyl...)
  7. Domain IPR009080
    Aminoacyl-tRNA synthetase, class Ia, anticodon-binding
    1. G3DSA:1.10.73...
    2. SSF47323 (Anticodon...)
  8. SITE IPR001412
    Aminoacyl-tRNA synthetase, class I, conserved site
    1. PS00178 (AA_TRNA_LI...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. CYTOPLASMIC_D... (C...)
  2. Coil
  3. G3DSA:2.170.2...
  4. G3DSA:2.20.28.20
  5. NON_CYTOPLASM... (N...)
  6. SSF52374 (Nucleotid...)
  7. TMhelix
  8. TRANSMEMBRANE (Tran...)
  9. cd07957 (Anticodon_...)

Residue annotation

  1. active site cd00814
  2. HIGH motif cd00814
  3. KMSKS motif cd00814
  4. tRNA binding surfa...
  5. anticodon binding ...

Protein sequence

>MCA_01103_1
MSKFTQVLHLPSASEGLVVAQSIKAVIAKNVSASKLSVVAEEDAKRVSFDLTPESSLIEANAIVRYLVSESQLESELIAF
EESTIYSTLKSQSKDVDFSKYPIPSKVQLTVGQIIFFGGLYGLVHNVGLKALESNAKLASWYAEFSKEKVIEQSIKEVAK
NTVKKSAPLPEYTGKANVDPSSLYIKPEGKITPKAGERNILITSALPYVNNVPHLGNIVGSVLSADIYARYSKQRGYNTL
FVCGTDEYGTATETKALEEKVTPQELCNKYHAIHKETYDWFQIGFDYFGRTTTPKQTKIAQDIFLKLNENGFLEEQSMEQ
LYCPEHKGFLADRFVEGTCPKCGYEDARGDQCDGCGNLLNPMELIKPRCKIDNGTPEKRNSNHIFLLLNKLQERVEKWVN
KSEVEGNWSMNSRTITHSWLKQGLHERCITRDLKWGTPVPLASYEDKVLYVWFDACIGYVSITANYIDDWEQWWRNPENV
KLYQFMGKDNVPFHTVVFPASQFGTEEKWTMLHHLSTTEYLQYEGGKFSKSRNVGVFGNQAKETKVPQSVWRYYLASSRP
ETSDTQFSWDEFLAANNNELLPNLGNFVNRLVKFVAAKQNKVIPQYDTSFVDFASVKKDLDDLLNTYNKAMEETHLRKGL
ETAMAISSRGNAFLQENKLDNSLLENHPEKAAAVIGVGINIIYLVSAVFAPFIPETSENILKQLNAPPILIPDEFKLNIL
GGHNIGKPAYLFSRIDSSKVDEWRSKFGGKKN

GO term prediction

Biological Process

GO:0006418 tRNA aminoacylation for protein translation
GO:0006431 methionyl-tRNA aminoacylation

Molecular Function

GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004825 methionine-tRNA ligase activity
GO:0005524 ATP binding

Cellular Component

GO:0005737 cytoplasm