Protein

MCA_00986_1

Length
2,130 amino acids


Gene name: GLT1

Description: Glutamate synthase [NADH]

Browser: contigA:3135510-3141903+

RNA-seq: read pairs 6282, FPKM 36.4, percentile rank 58.2% (100% = highest expression)

Protein function

Annotation:GLT1Glutamate synthase [NADH]
KEGG:K00264GLT1 glutamate synthase (NADPH/NADH) [EC:1.4.1.13 1.4.1.14]
EGGNOG:0PGFWGLT1glutamate synthase
SGD closest match:S000002330GLT1Glutamate synthase [NADH]
CGD closest match:CAL0000182870GLT1Glutamate synthase (NADH)

Protein alignments

%idAln lengthE-value
MIA_05201_190.52%21210.0MIA_05201_1
A0A0J9X9S4_GEOCN85.43%21140.0Similar to Saccharomyces cerevisiae YDL171C GLT1 NAD(+)-dependent glutamate synthase (GOGAT) OS=Geotrichum candidum GN=BN980_GECA06s01880g PE=4 SV=1
A0A060T400_BLAAD81.45%21190.0ARAD1C37774p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C37774g PE=4 SV=1
A0A167EHB1_9ASCO81.86%21280.0Glutamate synthase (NADH) OS=Sugiyamaella lignohabitans GN=GLT1 PE=4 SV=1
A0A1E3PSI4_9ASCO81.23%21200.0Glutamate synthase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_81242 PE=4 SV=1
Q6CDZ4_YARLI79.77%21300.0YALI0B19998p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_B19998g PE=4 SV=1
A0A1E4TGN6_9ASCO76.94%21210.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_31714 PE=4 SV=1
A0A1D8PDU9_CANAL74.04%21190.0Glutamate synthase (NADH) OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=GLT1 PE=4 SV=1
UniRef50_A0A179GPQ573.60%21290.0Glutamate synthase n=8 Tax=Hypocreales TaxID=5125 RepID=A0A179GPQ5_9HYPO
GLT1_YEAST64.59%21320.0Glutamate synthase [NADH] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GLT1 PE=1 SV=2

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0020

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 200 400 600 800 1000 1200 1400 1600 1800 2000 2130

Detailed signature matches

    1. SSF56235 (N-termina...)
    1. PF00310 (GATase_2)
    2. PS51278 (GATASE_TYPE_2)
    1. PF04898 (Glu_syn_ce...)
    1. PF01645 (Glu_synthase)
    2. cd02808 (GltS_FMN)
    1. PF01493 (GXGXG)
    2. SSF69336 (Alpha sub...)
    1. SSF46548 (alpha-hel...)
    1. PF14691 (Fer4_20)
    1. PF07992 (Pyr_redox_2)
Unintegrated signatures no IPR
Unintegrated signatures
  1. PR00419 (ADXRDTASE)
  2. SSF51395 (FMN-linke...)
  3. SSF51971 (Nucleotid...)
  4. cd00713 (GltS)
  5. cd00982 (gltB_C)
  6. mobidb-lite (disord...)

Residue annotation

  1. active site cd00713
  2. dimer interface cd...
  3. FMN binding site c...
  4. active site cd02808
  5. substrate binding ...
  6. 3Fe-4S cluster bin...
  7. domain interface c...

Protein sequence

>MCA_00986_1
METINNEEINEQYAPYDYVNTPENNSWAGALPVKQGLYDPEFEKDACGVGFSCHIKGKVSHKIVSDAKDLLCKMTHRGAV
GADARDGDGAGVMTSIPHKFMVKQFETTNDFKLPPLGQYAVGNLFFKNDEQVLEQSRNILERIAESLGLRVLGWREVPRD
SSLLGPAALSREPLILQPIVVLTEAYGSGNSPETTDAELFEKNFERTFERKLYILRKQSTHSIGLHNWFYICSLSNKNIV
YKGQLAPVQVYQYYYDLANVDYEAHFALVHSRFSTNTFPSWDRAQPLRWAAHNGEINTLRGNKNWMRAREGVMKSDVFGE
ELDKLYPIIEEGGSDSAAFDNVLELLVMNGVLSLPEAVMIMVPEAWQNNTDMNPKKRAFYEWAACLMEPWDGPALFTFAD
GRYCGANLDRNGLRPCRYYVTDDDRMICASEVGVMDIPPESVIQKGRLRPGRMLLVDTKEGIIVDDRELKRRVSERFDFK
SWASANLLTMPDLFAKLEAREGANAIESLKPKLDSFTVQNDPRLKAFGYTFEQVSLLLAPMAAEGKEALGSMGNDGPLAC
LAPQPRLLYEYFRQLFAQVTNPPIDPIRESIVMSLECYIGPQGNVLEMKPNQMDRLLMPSPILSNVEFAGLSLLPKFYPS
WTIDFIDITFPKSEGVAGYTRALERIAQEASEAITNHNKIIVLTDRAVNADRVAVSALLATGAVHHHLVRQKQRSKVALV
VETGEAREVHHMCVLVGYGADAINPYLAIETLLKMNREGLLKQQLDDKTIVENYKHSIDGGILKVMSKMGISTLASYKGA
QIFEALGIDNSVVDKCFTGTASRIKGITFDYIAEDAFTIHERGFPSRLTIKPEGLPESGEYHYRDGGDAHMNTPVAIANI
QDAVRNKNEKAYETYSKNEYEATKNCTLRGLLDFDFDNSQAIPIDQVEPWTEIVRRFVTGAMSYGSISMESHSTLAVAMN
RLGGKSNTGEGGEDPERSNVNEQGDTMRSAIKQIASGRFGVTSYYLSDSDELQIKMAQGAKPGEGGELPGDKVSQSIAKT
RHSTPGVGLISPPPHHDIYSIEDLKQLIYDLKCSNPRARVSVKLVSEVGVGIIASGVAKAKADHILISGHDGGTGASRWT
GIKYAGLPWELGLAETHQTLVLNDLRGRVIVQTDGQIKTGRDVAIACLLGAEEWGFATTPLIAMGCIMMRKCHLNTCPVG
IATQDPELRKKFMGTPEHVINFFYYIANELRQIMAKLGYRTINEMVGHAEKLKVRDDLRNSKTINLDLTPLLTPAHTLRP
GVATRNVRKQDHRLHVRLDNKLVDEAEVTLEKGLPVNIEAQIVNTDRALGTTLSYRISKKFGEQGLPNDTAHVYITGSAG
QSFGAFLAPGITLELEGDANDYVGKGLSGGRIIIYPPTNSPFKAEENIIIGNTCLYGATSGTCFFRGVAAERFAVRNSGA
TAVVEGCGDHGCEYMTGGRVVVLGPTGRNFAAGMSGGIAYVLDMAQEFNSNVNTEMVELCPLTDPAEIAFVRGLIEDHRH
YTNSELADRVLTDFNRILPRFVKVFPTDYKRVLEQEKKKKEELKKAEVVDFFHNIREDPDADITNGEILRTRTPAPITGT
NTPVAKKEPKVLDLEDTILDHAAEKKKAAVLKLDKTRGFKLYKRRNEKYRPATDRTKDWAELSNRLTKEELKYQTARCMD
CGVPFCQSDTGCPISNIIPKWNELVFKDRWYDALQRLLMTNNFPEFTGRVCPAPCEGACVLGINEDPVGIKSVEAAIIDH
GWAQGWIKPNPPPVRTGKSVAIIGSGPAGLACADQLNRAGHSVTVYERADRVGGLLMYGIPNMKLDKRVVERRTNLMRDE
GVKFITNTTIGEDITVEELESQNDAVVFACGSTIARDLKIPGRELNNIMYAMQLLKWNTKALLEDNLEEVRSLLHGKNVV
VIGGGDTGNDCIGTSVRHGAKSVVNFELLPKPPAARGKDNPWPQWPRIFRVDYGHTEVTAHYGKDPREYCILSKQFVGDE
DGNVKGIKTVRVEWKKSESGAWQMSEVPNSEEFFPADVVLLSMGFIGPEAQNLGVERDPRGNIKTTGPSSYRIGDKFFAA
GDCRRGQSLIVWGIQEGRQCARDIDIFLVGNTRLPGNGSIEQRDFEIAAK

GO term prediction

Biological Process

GO:0006537 glutamate biosynthetic process
GO:0006807 nitrogen compound metabolic process
GO:0008152 metabolic process
GO:0055114 oxidation-reduction process

Molecular Function

GO:0003824 catalytic activity
GO:0005506 iron ion binding
GO:0010181 FMN binding
GO:0015930 glutamate synthase activity
GO:0016040 glutamate synthase (NADH) activity
GO:0016491 oxidoreductase activity
GO:0016638 oxidoreductase activity, acting on the CH-NH2 group of donors
GO:0016639 oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor
GO:0045181 glutamate synthase activity, NAD(P)H as acceptor
GO:0050660 flavin adenine dinucleotide binding
GO:0051536 iron-sulfur cluster binding

Cellular Component

None predicted.