MCA_00807_1

Protein

Length

361 amino acids

RNA-seq: read pairs 149, FPKM 5.1, percentile rank 17.5% (100% = highest expression)

Protein function

KEGG:	K00002	AKR1A1	alcohol dehydrogenase (NADP+) [EC:1.1.1.2]
EGGNOG:	0PG90		alcohol dehydrogenase
SGD closest match:	S000000702	ADH7	NADP-dependent alcohol dehydrogenase 7
CGD closest match:	CAL0000187810	orf19.5517	NADP-dependent alcohol dehydrogenase

Protein alignments

	%id	Aln length	E-value
MIA_03123_1	59.00%	361	3e-136	MIA_03123_1
A0A1E4TMD6_9ASCO	55.52%	362	1e-127	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_95529 PE=3 SV=1
UniRef50_A0A1Q2YFY7	49.72%	354	2e-93	Uncharacterized protein n=1 Tax=Pichia membranifaciens TaxID=4926 RepID=A0A1Q2YFY7_9ASCO
A0A1E3PTC8_9ASCO	43.49%	361	1e-88	GroES-like protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_49175 PE=3 SV=1
ADH7_YEAST	45.05%	364	3e-81	NADP-dependent alcohol dehydrogenase 7 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ADH7 PE=1 SV=1
A0A167CUK7_9ASCO	39.50%	362	3e-65	Adh6p OS=Sugiyamaella lignohabitans GN=ADH6 PE=3 SV=1
Q5AC33_CANAL	36.61%	366	1e-52	NADP-dependent alcohol dehydrogenase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.5517 PE=3 SV=1
A0A060TH34_BLAAD	27.55%	363	2e-16	ARAD1D27874p OS=Blastobotrys adeninivorans GN=AADH3 PE=3 SV=1
ADH2_YARLI	26.61%	327	2e-14	Alcohol dehydrogenase 2 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=ADH2 PE=1 SV=1
A0A0J9XJK3_GEOCN	26.01%	323	2e-13	Similar to Saccharomyces cerevisiae YMR083W ADH3 Mitochondrial alcohol dehydrogenase isozyme III OS=Geotrichum candidum GN=BN980_GECA19s01275g PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0242

Protein family membership

None predicted.

Domains and repeats

Domain
5 - 352
Polyketide synthase, enoylreductase domain (IPR020843)
Domain
21 - 176
GroES-like (IPR011032)
Alcohol dehydrogenase, N-terminal (IPR013154)
190 - 315
Alcohol dehydrogenase, C-terminal (IPR013149)
Domain
153 - 321
NAD(P)-binding domain (IPR016040)

1 50 100 150 200 250 300 361

Detailed signature matches

IPR020843

Polyketide synthase, enoylreductase domain
1. SM00829 (PKS_ER_nam...)
  
  5 - 352
  
  SMART
  SM00829 (PKS_ER_names_mod)
IPR011032

GroES-like
1. SSF50129 (GroES-like)
  
  21 - 176
  
  SUPERFAMILY
  SSF50129 (GroES-like)
IPR013154

Alcohol dehydrogenase, N-terminal
1. PF08240 (ADH_N)
  
  35 - 148
  
  Pfam
  PF08240 (ADH_N)
IPR016040

NAD(P)-binding domain
1. SSF51735 (NAD(P)-bi...)
  
  153 - 321
  
  SUPERFAMILY
  SSF51735 (NAD(P)-binding Rossmann-fold domains)
IPR013149

Alcohol dehydrogenase, C-terminal
1. PF00107 (ADH_zinc_N)
  
  190 - 315
  
  Pfam
  PF00107 (ADH_zinc_N)
IPR002328

Alcohol dehydrogenase, zinc-type, conserved site
1. PS00059 (ADH_ZINC)
  
  67 - 81
  
  PROSITE patterns
  PS00059 (ADH_ZINC)

no IPR

Unintegrated signatures

G3DSA:3.40.50.20

197 - 353

GENE3D
G3DSA:3.40.50.20 (G3DSA:3.40.50.20)
G3DSA:3.90.18...

1 - 196

GENE3D
G3DSA:3.90.180.10 (G3DSA:3.90.180.10)
cd05283 (CAD1)

20 - 353

CDD
cd05283 (CAD1)

Residue annotation

catalytic Zn bindi...

46C

catalytic Zn binding site
46C, 68H, 163C
CDD
cd05283 (CAD1)

68H

catalytic Zn binding site
46C, 68H, 163C
CDD
cd05283 (CAD1)

163C

catalytic Zn binding site
46C, 68H, 163C
CDD
cd05283 (CAD1)
putative substrate...

46C

putative substrate binding site
46C, 48S, 68H, 94P, 163C, 302T
CDD
cd05283 (CAD1)

48S

putative substrate binding site
46C, 48S, 68H, 94P, 163C, 302T
CDD
cd05283 (CAD1)

68H

putative substrate binding site
46C, 48S, 68H, 94P, 163C, 302T
CDD
cd05283 (CAD1)

94P

putative substrate binding site
46C, 48S, 68H, 94P, 163C, 302T
CDD
cd05283 (CAD1)

163C

putative substrate binding site
46C, 48S, 68H, 94P, 163C, 302T
CDD
cd05283 (CAD1)

302T

putative substrate binding site
46C, 48S, 68H, 94P, 163C, 302T
CDD
cd05283 (CAD1)
putative NAD(P) bi...

46C

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

47G

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

48S

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

51H

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

163C

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

167T

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

210S

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

211R

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

215K

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

230T

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

249T

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

250G

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

254S

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

274I

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

275T

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

300T

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

301C

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)

302T

putative NAD(P) binding site
46C, 47G, 48S, 51H, 163C, 167T, 210S, 211R, 215K, 230T, 249T, 250G, 254S, 274I, 275T, 300T, 301C, 302T
CDD
cd05283 (CAD1)
structural Zn bind...

99D

structural Zn binding site
99D, 102C, 105C, 113C
CDD
cd05283 (CAD1)

102C

structural Zn binding site
99D, 102C, 105C, 113C
CDD
cd05283 (CAD1)

105C

structural Zn binding site
99D, 102C, 105C, 113C
CDD
cd05283 (CAD1)

113C

structural Zn binding site
99D, 102C, 105C, 113C
CDD
cd05283 (CAD1)
dimer interface cd...

112Y

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

170T

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

174E

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

268R

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

273F

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

274I

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

275T

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

277P

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

288Q

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

290L

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

291L

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

294G

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

295I

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

296T

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

297I

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

298Q

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

299G

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

300T

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

301C

dimer interface
112Y, 170T, 174E, 268R, 273F, 274I, 275T, 277P, 288Q, 290L, 291L, 294G, 295I, 296T, 297I, 298Q, 299G, 300T, 301C
CDD
cd05283 (CAD1)

Protein sequence

>MCA_00807_1
MVYPETIEALGLTQWEDYPHPKKFEYKPQDMRDYDVDVEIECCAICGSDVHGAAGNWFKPYLPIAFGHEIVGKVVNVGPK
VKGLEIGDRVGVGPQVDCDHTCEYCVTRLENYCTNLVGTYLSTYPDTGHNTIGGNASHIRVHSDFAFKIPENMSSEHAAP
LLCGGITAFTPLLEANVGKGTKVGIIGIGGIGSMGIQIAKALGAEVTAISRSYNKKDDALKLGADHYIATSDTEAMEKYK
KSLEVVVYTGSSFSQTSIEGIFSLIKNRGQFKFITAPPSDQLLQLKPQPLLFAGITIQGTCTGSPDDIKRLLQLASEHNI
KPWIETFDINEKTLGECWEGVEQGKPRFRYVMTGYNKYFKK

GO term prediction

Biological Process

GO:0055114 oxidation-reduction process

Molecular Function

GO:0008270 zinc ion binding
GO:0016491 oxidoreductase activity

Cellular Component