Protein

MCA_00776_1

Length
595 amino acids

Gene name: MSE1

Description: Glutamate--tRNA ligase, mitochondrial

Browser: contigA:2419542-2421387-

RNA-seq: read pairs 866, FPKM 18.0, percentile rank 38.4% (100% = highest expression)

Protein function

Annotation:MSE1Glutamate--tRNA ligase, mitochondrial
KEGG:K01885EARS glutamyl-tRNA synthetase [EC:6.1.1.17]
EGGNOG:0PH8UMSE1Glutamyl-tRNA synthetase
SGD closest match:S000005393MSE1Glutamate--tRNA ligase, mitochondrial
CGD closest match:CAL0000186309CAALFM_CR03110WAGlutamate--tRNA ligase

Protein alignments

%idAln lengthE-value
MIA_01028_160.65%5870.0MIA_01028_1
A0A0J9XLI7_GEOCN58.67%5710.0Similar to Saccharomyces cerevisiae YOL033W MSE1 Mitochondrial glutamyl-tRNA synthetase OS=Geotrichum candidum GN=BN980_GECA32s01176g PE=3 SV=1
A0A167E2C9_9ASCO54.09%5750.0Glutamate--tRNA ligase MSE1 OS=Sugiyamaella lignohabitans GN=MSE1 PE=3 SV=1
A0A060T361_BLAAD52.58%5420.0ARAD1A13486p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A13486g PE=3 SV=1
A0A1E3PHB8_9ASCO51.64%5790.0Glutamyl-tRNA synthetase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_83548 PE=3 SV=1
UniRef50_A0A099P4W352.17%5312e-179Uncharacterized protein n=4 Tax=Pichiaceae TaxID=1156497 RepID=A0A099P4W3_PICKU
SYEM_YEAST48.50%5323e-167Glutamate--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MSE1 PE=1 SV=2
Q6C5H9_YARLI46.38%5523e-161YALI0E17919p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E17919g PE=3 SV=1
A0A1D8PSE8_CANAL45.41%5558e-160Glutamate--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=CAALFM_CR03110WA PE=3 SV=1
A0A1E4TJ79_9ASCO47.01%3511e-102Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_18760 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9892
Predicted cleavage: 31

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
  3. Domain
1 100 200 300 400 500 595

Detailed signature matches

  1. Family IPR000924
    Glutamyl/glutaminyl-tRNA synthetase
    1. PR00987 (TRNASYNTHGLU)
  2. Family IPR004527
    Glutamate-tRNA ligase, bacterial/mitochondrial
    1. MF_00022 (Glu_tRNA_...)
    2. TIGR00464
  3. Domain IPR014729
    Rossmann-like alpha/beta/alpha sandwich fold
    1. G3DSA:3.40.50...
  4. Domain IPR020058
    Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain
    1. PF00749 (tRNA-synt_1c)
  5. Domain IPR033910
    Glutamyl-tRNA synthetase
    1. cd00808 (GluRS_core)
  6. Domain IPR020061
    Glutamyl/glutaminyl-tRNA synthetase, class Ib, alpha-bundle domain
    1. G3DSA:1.10.11...
  7. Domain IPR008925
    Aminoacyl-tRNA synthetase, class I, anticodon-binding
    1. SSF48163 (An antico...)
  8. Domain IPR020751
    Aminoacyl-tRNA synthetase, class I, anticodon-binding domain, subdomain 2
    1. G3DSA:1.10.10...
Unintegrated signatures no IPR
Unintegrated signatures
  1. SSF52374 (Nucleotid...)
  2. mobidb-lite (disord...)

Residue annotation

  1. active site cd00808
  2. HIGH motif cd00808
  3. KMSKS motif cd00808

Protein sequence

>MCA_00776_1
MTSSFLISSRIRLVSSRSISFKSLTLSRLYSSKFSLKPKHSLFEESLKSSTSKANNHHSPSETNSIQPTTPARTRFAPSP
TGYIHLGSLRTALYNYLLARSTGGQFLLRLEDTDQNRLVQGAEENIYKSLEWAGLKWDEGPIVGGPYGPYRQSERAEIYS
KYANQLIESGNAYRCFCSKDRLDSLRASARKLHPPSMASYDRKCSHLSSEEALDRVQKGQEYTIRFKAPLHYPEFIDLLH
GKLNMQTQVNPDDVRYDDPVLVKSDGLPTYHLANVVDDHLMKITHVVRGEEWLASTPKHVAMYKAFGWKPPSFVHIPLLT
TTANKKLSKRAGDIGVLSLAEKGILPEAMINYVALYGWSPSSTSPNSDIFTLKELEKAFSLNGLTRGNAKVDEKKLIFLN
KHFFQVELNNDPKKFNQFLDTIHSQISEKLADTEQDSLDVSKSLLTDKEYTLRILNLIKTSITSVEDFIERTKPLYYPPT
FDLTESSSSASKYLSKLQKDGDVDKILIPVLTETTQVMNKMGGSEYWANFDNAKEFCNHGLLEHFSQFKKPQVFQALRFG
LSGNVPGIDIPTIIYILGPEVVSKRFETALKVING

GO term prediction

Biological Process

GO:0006418 tRNA aminoacylation for protein translation
GO:0006424 glutamyl-tRNA aminoacylation
GO:0043039 tRNA aminoacylation

Molecular Function

GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004818 glutamate-tRNA ligase activity
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016876 ligase activity, forming aminoacyl-tRNA and related compounds

Cellular Component

GO:0005737 cytoplasm