MCA_00695_1

Protein

Length

1,152 amino acids

Gene name: CPA2

Description: Carbamoyl-phosphate synthase arginine-specific large chain

Browser: contigA:2179440-2183079-

RNA-seq: read pairs 8947, FPKM 95.9, percentile rank 77.9% (100% = highest expression)

Protein function

Annotation:		CPA2	Carbamoyl-phosphate synthase arginine-specific large chain
KEGG:	K01955	carB	carbamoyl-phosphate synthase large subunit [EC:6.3.5.5]
EGGNOG:	0PH1U	CPA2	carbamoylphosphate synthase
SGD closest match:	S000003870	CPA2	Carbamoyl-phosphate synthase arginine-specific large chain
CGD closest match:	CAL0000195469	CPA2	Carbamoyl-phosphate synthase (Glutamine-hydrolyzing)

Protein alignments

	%id	Aln length
MIA_01114_1	90.55%	1132	MIA_01114_1
A0A0J9X970_GEOCN	88.32%	1122	Similar to Saccharomyces cerevisiae YJR109C CPA2 Large subunit of carbamoyl phosphate synthetase OS=Geotrichum candidum GN=BN980_GECA05s07259g PE=4 SV=1
A0A167FKF2_9ASCO	82.21%	1141	Carbamoyl-phosphate synthase (Glutamine-hydrolyzing) CPA2 OS=Sugiyamaella lignohabitans GN=CPA2 PE=4 SV=1
A0A060THU6_BLAAD	81.21%	1128	ARAD1D34210p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D34210g PE=4 SV=1
A0A1E3PLV1_9ASCO	81.61%	1115	Carbamoyl-phosphate synthase large subunit OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_23269 PE=4 SV=1
A0A1E4TCT1_9ASCO	78.26%	1141	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_140920 PE=4 SV=1
Q5A8A6_CANAL	78.28%	1142	Carbamoyl-phosphate synthase (Glutamine-hydrolyzing) OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=CPA2 PE=4 SV=1
UniRef50_B8MHQ3	77.35%	1130	Carbamoyl-phosphate synthase, large subunit n=8 Tax=Trichocomaceae TaxID=28568 RepID=B8MHQ3_TALSN
Q6CAV8_YARLI	77.65%	1141	YALI0C23969p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_C23969g PE=4 SV=1
CARB_YEAST	73.93%	1097	Carbamoyl-phosphate synthase arginine-specific large chain OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CPA2 PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9946
Predicted cleavage: 42

Protein family membership

Carbamoyl-phosphate synthase, large subunit (IPR006275)

Domains and repeats

Domain
69 - 190
Pre-ATP-grasp domain (IPR016185)
455 - 608
Carbamoyl-phosphate synthetase, large subunit oligomerisation domain (IPR005480)
610 - 729
Pre-ATP-grasp domain (IPR016185)
987 - 1,125
Methylglyoxal synthase-like domain (IPR011607)
Domain
82 - 96
Carbamoyl-phosphate synthase large subunit, CPSase domain (IPR005483)
111 - 121
Carbamoyl-phosphate synthase large subunit, CPSase domain (IPR005483)
191 - 394
ATP-grasp fold (IPR011761)
Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain (IPR005479)
Carbamoyl-phosphate synthase large subunit, CPSase domain (IPR005483)
439 - 457
Carbamoyl-phosphate synthase large subunit, CPSase domain (IPR005483)
727 - 930
ATP-grasp fold (IPR011761)
Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain (IPR005479)
Domain
709 - 798
ATP-grasp fold, subdomain 1 (IPR013815)

1 200 400 600 800 1000 1152

Detailed signature matches

IPR006275

Carbamoyl-phosphate synthase, large subunit
1. TIGR01369
  
  69 - 1,122
  
  TIGRFAMs
  TIGR01369 (TIGR01369)
IPR016185

Pre-ATP-grasp domain
1. SSF52440 (PreATP-gr...)
  
  610 - 729
  
  SUPERFAMILY
  SSF52440 (PreATP-grasp domain)
  
  69 - 190
  
  SUPERFAMILY
  SSF52440 (PreATP-grasp domain)
IPR011761

ATP-grasp fold
1. PS50975 (ATP_GRASP)
  
  196 - 388
  
  PROSITE profiles
  PS50975 (ATP_GRASP)
  
  732 - 930
  
  PROSITE profiles
  PS50975 (ATP_GRASP)
IPR005479

Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain
1. PF02786 (CPSase_L_D2)
  
  727 - 927
  
  Pfam
  PF02786 (CPSase_L_D2)
  
  191 - 394
  
  Pfam
  PF02786 (CPSase_L_D2)
2. PS00867 (CPSASE_2)
  
  899 - 906
  
  PROSITE patterns
  PS00867 (CPSASE_2)
  
  357 - 364
  
  PROSITE patterns
  PS00867 (CPSASE_2)
3. PS00866 (CPSASE_1)
  
  227 - 241
  
  PROSITE patterns
  PS00866 (CPSASE_1)
  
  763 - 777
  
  PROSITE patterns
  PS00866 (CPSASE_1)
IPR005483

Carbamoyl-phosphate synthase large subunit, CPSase domain
1. PR00098 (CPSASE)
  
  231 - 243
  
  PRINTS
  PR00098 (CPSASE)
  
  357 - 386
  
  PRINTS
  PR00098 (CPSASE)
  
  300 - 317
  
  PRINTS
  PR00098 (CPSASE)
  
  82 - 96
  
  PRINTS
  PR00098 (CPSASE)
  
  265 - 284
  
  PRINTS
  PR00098 (CPSASE)
  
  439 - 457
  
  PRINTS
  PR00098 (CPSASE)
  
  111 - 121
  
  PRINTS
  PR00098 (CPSASE)
IPR005480

Carbamoyl-phosphate synthetase, large subunit oligomerisation domain
1. SSF48108 (Carbamoyl...)
  
  455 - 608
  
  SUPERFAMILY
  SSF48108 (Carbamoyl phosphate synthetase, large subunit connection domain)
2. PF02787 (CPSase_L_D3)
  
  480 - 601
  
  Pfam
  PF02787 (CPSase_L_D3)
3. SM01096 (CPSase_L_D3_2)
  
  476 - 601
  
  SMART
  SM01096 (CPSase_L_D3_2)
4. G3DSA:1.10.10...
  
  460 - 592
  
  GENE3D
  G3DSA:1.10.1030.10 (G3DSA:1.10.1030.10)
IPR013815

ATP-grasp fold, subdomain 1
1. G3DSA:3.30.14...
  
  709 - 798
  
  GENE3D
  G3DSA:3.30.1490.20 (G3DSA:3.30.1490.20)
IPR011607

Methylglyoxal synthase-like domain
1. G3DSA:3.40.50...
  
  987 - 1,125
  
  GENE3D
  G3DSA:3.40.50.1380 (G3DSA:3.40.50.1380)
2. SSF52335 (Methylgly...)
  
  998 - 1,125
  
  SUPERFAMILY
  SSF52335 (Methylglyoxal synthase-like)

no IPR

Unintegrated signatures

G3DSA:3.30.47...

183 - 459

GENE3D
G3DSA:3.30.470.20 (G3DSA:3.30.470.20)

799 - 986

GENE3D
G3DSA:3.30.470.20 (G3DSA:3.30.470.20)
G3DSA:3.40.50.20

593 - 708

GENE3D
G3DSA:3.40.50.20 (G3DSA:3.40.50.20)

68 - 180

GENE3D
G3DSA:3.40.50.20 (G3DSA:3.40.50.20)
NON_CYTOPLASM... (N...)

19 - 1,152

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
SIGNAL_PEPTIDE (Sig...)

1 - 18

PHOBIUS
SIGNAL_PEPTIDE (Signal Peptide)
SIGNAL_PEPTID... (S...)

14 - 18

PHOBIUS
SIGNAL_PEPTIDE_C_REGION (Signal peptide C-region)
SIGNAL_PEPTID... (S...)

5 - 13

PHOBIUS
SIGNAL_PEPTIDE_H_REGION (Signal peptide H-region)
SIGNAL_PEPTID... (S...)

1 - 4

PHOBIUS
SIGNAL_PEPTIDE_N_REGION (Signal peptide N-region)
SSF56059 (Glutathio...)

727 - 994

SUPERFAMILY
SSF56059 (Glutathione synthetase ATP-binding domain-like)

191 - 459

SUPERFAMILY
SSF56059 (Glutathione synthetase ATP-binding domain-like)
cd01423 (MGS_CPS_I_III)

1,005 - 1,122

CDD
cd01423 (MGS_CPS_I_III)

Residue annotation

probable substrate...

1,010G

probable substrate binding site
1,010G, 1,016D, 1,035A, 1,037P, 1,038A, 1,083N, 1,084L, 1,086K
CDD
cd01423 (MGS_CPS_I_III)

1,016D

probable substrate binding site
1,010G, 1,016D, 1,035A, 1,037P, 1,038A, 1,083N, 1,084L, 1,086K
CDD
cd01423 (MGS_CPS_I_III)

1,035A

probable substrate binding site
1,010G, 1,016D, 1,035A, 1,037P, 1,038A, 1,083N, 1,084L, 1,086K
CDD
cd01423 (MGS_CPS_I_III)

1,037P

probable substrate binding site
1,010G, 1,016D, 1,035A, 1,037P, 1,038A, 1,083N, 1,084L, 1,086K
CDD
cd01423 (MGS_CPS_I_III)

1,038A

probable substrate binding site
1,010G, 1,016D, 1,035A, 1,037P, 1,038A, 1,083N, 1,084L, 1,086K
CDD
cd01423 (MGS_CPS_I_III)

1,083N

probable substrate binding site
1,010G, 1,016D, 1,035A, 1,037P, 1,038A, 1,083N, 1,084L, 1,086K
CDD
cd01423 (MGS_CPS_I_III)

1,084L

probable substrate binding site
1,010G, 1,016D, 1,035A, 1,037P, 1,038A, 1,083N, 1,084L, 1,086K
CDD
cd01423 (MGS_CPS_I_III)

1,086K

probable substrate binding site
1,010G, 1,016D, 1,035A, 1,037P, 1,038A, 1,083N, 1,084L, 1,086K
CDD
cd01423 (MGS_CPS_I_III)

Protein sequence

>MCA_00695_1
MIVRSSLASASSIRAAVASSICKTQLRRSCKSLFASSFVRHNSTNVNTAAKYAGAGILNPFQDKDGLVDVTNVLVIGSGG
LSIGQAGEFDYSGSQAIKALKEENKKSILINPNIATIQTSHGLADEVYFLPVTAEYIIDIIEREKPDGILLTFGGQTGLN
VGVQLDKMGVFERYGVKVLGTPIKTLETSEDRDLFAQALKEIDIPIAESIAVDTVDDALAAAEKVGYPIIVRSAYALGGL
GSGFANNKEELHNLASQSLSLAPQILVEKSLKGWKEVEYEVVRDRVGNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDE
EYHMLRSAAIKIIRHLGVVGECNVQYALQPDGLDFRVIEVNARLSRSSALASKATGYPLAYTAAKIALGHTLPELPNAVT
KTTSANFEPSLDYMVTKIPRWDLAKFQHVKRDIGSSMKSVGEVMAIGRTFEESFQKAIRQVDPSFVGFQGAEFEDLDFAL
ANPTDRRWLAVGQALLHEGYTVERVHELSKIDKWFLYKLMNIVNHQKSLEAIGSLFGITHDVMLTSKKLGFSDKQIALAV
GCTEDEVRARRLSFGIRPFVKKIDTLAAEFPADTNYLYTTYNATTHDVSFDEHGTMVLGSGVYRIGSSVEFDWCAVNTAR
SLRANGKKTIMINYNPETVSTDFDEVDRLYFEELSYERVMDIYELEQSKGVVVSVGGQLPQNIALKLKDGGANVLGTDPY
DIDKAEDRHKFSSILDSIGVDQPAWKELSSVGDAKIFADKVGYPVLVRPSYVLSGAAMSVINDESELEAKLTNAASVSPD
HPVVITKFIEGAEEIDVDAVASNGKLLVHAVSEHVENAGVHSGDATLVLPPVSLTETDMARLKEIAVKVAAAWKITGPFN
MQIIKAKNPDNATYPDLKVIECNIRASRSFPFVSKVLGTNFIDVATRALLAKDVPEPVDLMAQPRNYVATKVPQFSFTRL
AGADPFLGVEMASTGEVACFGKDLKEAYWISMQSTMNFHLPLPPNGILFGGDLTNDNLSKVARTMAPLGYKIYTASPAVK
EYLEKGFGDEASKYPVTVIEFPKSDKRKLREVFEKYSIKAVFNLAKKRAEDLLDEDYVMRRNAIDFAIPLFNEPNTSILF
AESVAEKLPLKQEGVIPSEVKRWSEWIGGKNV

GO term prediction

Biological Process

GO:0006807 nitrogen compound metabolic process

Molecular Function

GO:0003824 catalytic activity
GO:0005524 ATP binding
GO:0046872 metal ion binding

Cellular Component

None predicted.

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Entry type

Status

Per-residue features