Protein

MCA_00615_1

Length
960 amino acids

Gene name: ALA1

Description: Alanine--tRNA ligase

Browser: contigA:1886705-1889782-

RNA-seq: read pairs 7462, FPKM 95.9, percentile rank 78.0% (100% = highest expression)

Protein function

Annotation:ALA1Alanine--tRNA ligase
KEGG:K01872AARS alanyl-tRNA synthetase [EC:6.1.1.7]
EGGNOG:0PHIIALA1alanyl-tRNA synthetase
SGD closest match:S000005862ALA1Alanine--tRNA ligase, mitochondrial
CGD closest match:CAL0000182169ALA1Alanine--tRNA ligase

Protein alignments

%idAln lengthE-value
MIA_01382_184.25%9590.0MIA_01382_1
S4SV98_GEOCN76.72%9620.0Alanyl-tRNA synthetase (Fragment) OS=Geotrichum candidum GN=Ala1 PE=3 SV=1
Q6CFS9_YARLI73.87%9530.0Alanine--tRNA ligase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=ALA1 PE=3 SV=1
A0A060T6K1_BLAAD72.11%9610.0Alanine--tRNA ligase OS=Blastobotrys adeninivorans GN=ALA1 PE=3 SV=1
SYA_CANAL68.05%9640.0Alanine--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=ALA1 PE=1 SV=1
A0A161HI30_9ASCO73.74%9140.0Alanine--tRNA ligase OS=Sugiyamaella lignohabitans GN=ALA1 PE=3 SV=1
UniRef50_A0A099P4F169.51%9510.0Alanine--tRNA ligase n=2 Tax=Pichia kudriavzevii TaxID=4909 RepID=A0A099P4F1_PICKU
A0A1E3PFM0_9ASCO70.37%9080.0Alanine--tRNA ligase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=ALA1 PE=3 SV=1
SYA_YEAST65.90%9560.0Alanine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ALA1 PE=1 SV=3
A0A1E4TCY2_9ASCO67.08%9630.0Alanine--tRNA ligase OS=Tortispora caseinolytica NRRL Y-17796 GN=ALA1 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0699

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
  3. Domain
1 100 200 300 400 500 600 700 800 900 960

Detailed signature matches

  1. Family IPR002318
    Alanine-tRNA ligase, class IIc
    1. PR00980 (TRNASYNTHALA)
    2. TIGR00344
  2. Family IPR023033
    Alanine-tRNA ligase, eukaryota/bacteria
    1. MF_00036_B (Ala_tRN...)
  3. Domain IPR018165
    Alanyl-tRNA synthetase, class IIc, core domain
    1. PS50860 (AA_TRNA_LI...)
  4. Domain IPR018164
    Alanyl-tRNA synthetase, class IIc, N-terminal
    1. PF01411 (tRNA-synt_2c)
  5. Domain IPR018162
    Alanine-tRNA ligase, class IIc, anti-codon-binding domain
    1. SSF101353 (Putative...)
  6. Domain IPR009000
    Translation protein, beta-barrel domain
    1. SSF50447 (Translati...)
  7. Domain IPR018163
    Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain
    1. SSF55186 (ThrRS/Ala...)
  8. Domain IPR012947
    Threonyl/alanyl tRNA synthetase, SAD
    1. PF07973 (tRNA_SAD)
    2. SM00863 (tRNA_SAD_4)
  9. Domain IPR003156
    DHHA1 domain
    1. PF02272 (DHHA1)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:3.30.93...
  3. G3DSA:3.30.98...
  4. SSF55681 (Class II ...)
  5. cd00673 (AlaRS_core)

Residue annotation

  1. motif 1 cd00673
  2. active site cd00673
  3. motif 2 cd00673
  4. motif 3 cd00673

Protein sequence

>MCA_00615_1
MSTSQLTPENWPATKVRDTFLDFFKSKEHTFVPSSSVIPYDDPTLLFANAGMNQYKPIFLGTVDPSSPLSKISRAANSQK
CIRAGGKHNDLEDVGKDSYHHTFFEMLGNWSFGDYFKKEAISWAWELLTKVYGLDPQRLYVTYFGGDEKQGLEPDLEAKQ
IWADMGVPEDHILPGSLKDNFWEMGEQGPCGPCSEIHYDRIGGRNAAHLVNMDDPNVLEVWNIVFIQYNREANGELRVLP
AQHIDTGMGFERLVSVLQDKLSNYDTDVFTPIFDKIQELTNARPYTGKFGKEDADGIDTAYRVVADHVRTLTFAIADGGY
PNNEGRGYVLRRILRRGARYVRKKFDVPIGNFFSRLAPTLIDQVSHIFPELTRDPETIFEILNEEEQSFSRTLDRGEKLF
EQYAETAKKNGEKKLNGKDVWRLYDTYGFPVDLTTLMAEEAGLEIDEAEFEKARLESKEASKGGKKSAGKELLKLDVHSL
ASLEQNDKVPRTDDHYKYLPDDIEATVTAIYADNKFVDSTKDISPESQLGLLLDKTNFYAEQGGQEYDTGKIVIDGESEF
EVENVQTYAGYVLHTGYLKYGDLKVGDKVVAGFDNLRRWPIRNNHTGTHILNFALKEVLGDGVDQKGSLVAPEKLRFDFS
HKAALSLKEVEEVENISNKCIEKNLPVYAKDVPLSEATKIAGVRAVFGETYPDPVRVVSVGVPVEDLIAKPENPEWEKYS
VEFCGGTHVAKTSDIKELVIIEESGIAKGIRRIVAVTGTTAYDVQKLAKAFDEKLDALESLPSGAEKENRSKNLGVELKK
LSVAVVSKAKLNEKYTKIQKKISDESKARQKAEGKKALSTVTEYFEKNADAPYLVLSVPEISANSKALNEAVNHIKAKQP
EKSVYLFAGEDGKVAHVCYIGAPAVEKGVSAQELGSIATKFVGGRSGGKGPTVQGAGTDNTKIPDAVSEISKLFKEKLSI

GO term prediction

Biological Process

GO:0006419 alanyl-tRNA aminoacylation
GO:0043039 tRNA aminoacylation

Molecular Function

GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0004813 alanine-tRNA ligase activity
GO:0005524 ATP binding
GO:0016876 ligase activity, forming aminoacyl-tRNA and related compounds

Cellular Component

GO:0005737 cytoplasm