Protein

MCA_00581_1

Length
502 amino acids

Gene name: DIA4

Description: Serine--tRNA ligase, mitochondrial

Browser: contigA:1794842-1796351-

RNA-seq: read pairs 1280, FPKM 31.4, percentile rank 54.0% (100% = highest expression)

Protein function

Annotation:DIA4Serine--tRNA ligase, mitochondrial
KEGG:K01875SARS seryl-tRNA synthetase [EC:6.1.1.11]
EGGNOG:0PHEHDIA4seryl-trna synthetase
SGD closest match:S000001053DIA4Serine--tRNA ligase, mitochondrial
CGD closest match:CAL0000178091orf19.1201Putative serine--tRNA ligase

Protein alignments

%idAln lengthE-value
MIA_00953_168.52%4860.0MIA_00953_1
A0A0J9XI39_GEOCN65.35%4820.0Similar to Saccharomyces cerevisiae YHR011W DIA4 Probable mitochondrial seryl-tRNA synthetase OS=Geotrichum candidum GN=BN980_GECA16s00384g PE=4 SV=1
UniRef50_K0KP5050.92%4891e-144Seryl-tRNA synthetase n=10 Tax=Saccharomycetales TaxID=4892 RepID=K0KP50_WICCF
A0A170QY06_9ASCO47.34%4881e-139Putative serine--tRNA ligase DIA4 OS=Sugiyamaella lignohabitans GN=DIA4 PE=4 SV=1
A0A060T4W1_BLAAD47.62%4622e-136ARAD1C01122p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C01122g PE=4 SV=1
A0A1E4TEM7_9ASCO45.29%4461e-129Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_103861 PE=4 SV=1
Q6CFL2_YARLI44.62%4462e-119YALI0B05918p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_B05918g PE=4 SV=1
Q59RK3_CANAL42.92%4665e-119Putative serine--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.1201 PE=4 SV=1
SYSM_YEAST45.03%4536e-118Serine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DIA4 PE=1 SV=1
A0A1E3PF42_9ASCO58.13%2469e-83Class II aaRS and biotin synthetase (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_84037 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.8866
Predicted cleavage: 35

Protein family membership

Domains and repeats

  1. Domain
1 50 100 150 200 250 300 350 400 450 502

Detailed signature matches

  1. Family IPR002317
    Serine-tRNA ligase, type1
    1. PR00981 (TRNASYNTHSER)
    2. TIGR00414
    3. PIRSF001529 (Ser-tR...)
  2. Domain IPR010978
    tRNA-binding arm
    1. SSF46589 (tRNA-bind...)
  3. Domain IPR006195
    Aminoacyl-tRNA synthetase, class II
    1. PS50862 (AA_TRNA_LI...)
  4. Domain IPR002314
    Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)
    1. PF00587 (tRNA-synt_2b)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:1.10.28...
  3. G3DSA:3.30.93...
  4. SSF55681 (Class II ...)

Protein sequence

>MCA_00581_1
MIRTVKLSIKRTLAFNPSLQVASFRSFSSSLCINDKNKKSDAKIKSTSALKKPHYDFKYIVANADKYLESAKLRNMTTKG
VEDIQSTYEPYQKARNQLNHLQAQRKQLESVFTASFGKNKGSSKEQNQQKPPIDIKEAKEKLNILKTEAKELSDKISDYE
QTLMIAGDLVPNLISPDSFVPEPQTVEILNDNNGSRPRLPDDFDHVAIGKKLGLIELEAASKVSGSSWYYLTGDGALLEQ
ALVSYAMSKARNHGFYPVIPPSIVRREVAGGCGFKPRDQNGEQQIYNLENSDLCLTGTAEIPLAGLYCDSVLEFPKEGPN
KGVKKVVGVSRSYRAEAGARGRDTKGLYRVHEFTKVELFAWADSLERSESVLSEILALEKEMISELGLTARVLNMSPDEL
GAPAYKKYDIEAWMPGRQDWGELTSTSNCTDFQARRMHTKYRNEEGKLVFVQTLNGTAMAVPRVIVAILETFYESDQNRV
KIPKVLQKWMDDREYITENRQL

GO term prediction

Biological Process

GO:0006418 tRNA aminoacylation for protein translation
GO:0006434 seryl-tRNA aminoacylation

Molecular Function

GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004828 serine-tRNA ligase activity
GO:0005524 ATP binding

Cellular Component

GO:0005737 cytoplasm