Protein

MCA_00505_1

Length
535 amino acids

Gene name: POB3

Description: FACT complex subunit POB3

Browser: contigA:1594976-1596584-

RNA-seq: read pairs 2775, FPKM 64.0, percentile rank 70.6% (100% = highest expression)

Protein function

Annotation:POB3FACT complex subunit POB3
KEGG:K09272SSRP1 structure-specific recognition protein 1
EGGNOG:0PHIHPOB3Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II
SGD closest match:S000004534POB3FACT complex subunit POB3
CGD closest match:CAL0000189327POB3FACT complex subunit POB3

Protein alignments

%idAln lengthE-value
A0A0J9XJA0_GEOCN66.99%5150.0FACT complex subunit POB3 OS=Geotrichum candidum GN=BN980_GECA23s00109g PE=3 SV=1
MIA_05800_167.71%5110.0MIA_05800_1
A0A060T964_BLAAD60.68%4730.0FACT complex subunit POB3 OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C41250g PE=3 SV=1
A0A1E3PL73_9ASCO59.05%4860.0FACT complex subunit POB3 OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_82614 PE=3 SV=1
POB3_YARLI54.06%5291e-174FACT complex subunit POB3 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=POB3 PE=3 SV=1
UniRef50_Q6C7V454.06%5293e-171FACT complex subunit POB3 n=4 Tax=Saccharomycetales TaxID=4892 RepID=POB3_YARLI
A0A1E4TCK4_9ASCO53.85%4681e-174FACT complex subunit POB3 (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_18662 PE=3 SV=1
POB3_CANAL51.38%5082e-172FACT complex subunit POB3 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=POB3 PE=3 SV=1
POB3_YEAST49.43%5242e-167FACT complex subunit POB3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=POB3 PE=1 SV=1
A0A167DAP8_9ASCO66.56%3143e-124FACT complex subunit POB3 OS=Sugiyamaella lignohabitans GN=POB3 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0291
Predicted cleavage: 22

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 50 100 150 200 250 300 350 400 450 500 535

Detailed signature matches

  1. Family IPR000969
    Structure-specific recognition protein
    1. PR00887 (SSRCOGNITION)
  2. Domain IPR011993
    PH domain-like
    1. G3DSA:2.30.29.30
    2. SSF50729 (PH domain...)
  3. Domain IPR035417
    FACT complex subunit POB3-like, N-terminal PH domain
    1. PF17292 (POB3_N)
  4. Domain IPR024954
    SSRP1 domain
    1. PF03531 (SSrecog)
  5. Domain IPR013719
    Domain of unknown function DUF1747
    1. PF08512 (Rtt106)
    2. SM01287 (Rtt106_2)
Unintegrated signatures no IPR
Unintegrated signatures
  1. cd13229 (PH_TFIIH)
  2. cd13230 (PH1_SSRP1-...)
  3. cd13231 (PH2_SSRP1-...)
  4. mobidb-lite (disord...)

Residue annotation

  1. peptide binding si...

Protein sequence

>MCA_00505_1
MPSQYENIFLNQSRVSGKVRLNPEGLGWKAAETKKTNVKAPETLLLSSGEIQAAFWSRAARGYEIRIQTKTKGAIQLDGF
DSEEFNALKTEFDNYYNVPLEVREHSLRGWNWGKADFGRNEMSFDVAGKPAFEISYADVSNSNLAGKNEVTVEFNLQSPN
ERLKAGDELVEIRFFVPGTVVNKTEDGEKNNDDDVQITEEEQSAATAFYEALKEKADIGDVAGEAIVSLPDIMFLTPRGR
YDIDMYSTSFGLRGKTYDYKIPYKNVQRLFLLPKPDEVHHLLVVQLDPPARQGQTKYPFFVIQFPREDELEVELNIDDAE
YEANYKNKLKKKYDEATHEVVGSLFRGLTNRKMMVPGSYKSTHGDAGVSCSLKASEGYLFVLETCFLFLPKPTVYIPISE
ISVITFSRIGSQTSSSSRTFDMAIALTRGGEHQFSNINKDEQKSLEDFIRSKGITIEMDLGDQPILRTLAEVSDSDGEID
RGSADEDEESPDEDFQAESESDVAEEFDSEADPSSSEDEGEDSEEESRIKKKPKN

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003677 DNA binding

Cellular Component

GO:0005634 nucleus