Protein

MCA_00463_1

Length
323 amino acids

Gene name: RPB3

Description: DNA-directed RNA polymerase II subunit RPB3

Browser: contigA:1461779-1462938-

RNA-seq: read pairs 2005, FPKM 76.5, percentile rank 74.5% (100% = highest expression)

Protein function

Annotation:RPB3DNA-directed RNA polymerase II subunit RPB3
KEGG:K03011RPB3 DNA-directed RNA polymerase II subunit RPB3
EGGNOG:0PG4TRPB3rna polymerase ii
SGD closest match:S000001283RPB3DNA-directed RNA polymerase II subunit RPB3
CGD closest match:CAL0000186561orf19.1248DNA-directed RNA polymerase II core subunit

Protein alignments

%idAln lengthE-value
MIA_00649_178.20%2668e-158MIA_00649_1
A0A0J9X5D4_GEOCN74.44%2661e-147Similar to Saccharomyces cerevisiae YIL021W RPB3 RNA polymerase II third largest subunit B44 OS=Geotrichum candidum GN=BN980_GECA02s03937g PE=4 SV=1
A0A060TFM9_BLAAD65.17%2679e-131ARAD1D24442p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D24442g PE=4 SV=1
A0A1E3PFS4_9ASCO67.16%2682e-130Putative DNA-directed RNA polymerase II subunit OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_47501 PE=4 SV=1
RPB3_YEAST61.28%2661e-121DNA-directed RNA polymerase II subunit RPB3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPB3 PE=1 SV=2
UniRef50_P1637061.28%2663e-118DNA-directed RNA polymerase II subunit RPB3 n=29 Tax=Saccharomycetales TaxID=4892 RepID=RPB3_YEAST
Q6C2S4_YARLI68.38%2343e-119YALI0F05566p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F05566g PE=4 SV=1
Q5A462_CANAL61.92%2604e-119DNA-directed RNA polymerase II core subunit OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.1248 PE=4 SV=1
A0A1E4TFX0_9ASCO67.14%2102e-101Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_31549 PE=4 SV=1
A0A161HM19_9ASCO71.43%1613e-79DNA-directed RNA polymerase II core subunit RPB3 OS=Sugiyamaella lignohabitans GN=RPB3 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0814

Protein family membership

None predicted.

Domains and repeats

  1. Domain
  2. Domain
  3. Domain
1 50 100 150 200 250 323

Detailed signature matches

  1. Domain IPR009025
    DNA-directed RNA polymerase, RBP11-like dimerisation domain
    1. SSF55257 (RBP11-lik...)
  2. Domain IPR011263
    DNA-directed RNA polymerase, RpoA/D/Rpb3-type
    1. SM00662 (rpoldneu2)
    2. PF01193 (RNA_pol_L)
  3. Domain IPR011262
    DNA-directed RNA polymerase, insert domain
    1. PF01000 (RNA_pol_A_bac)
    2. SSF56553 (Insert su...)
    3. G3DSA:2.170.1...
  4. SITE IPR001514
    DNA-directed RNA polymerase, 30-40kDa subunit, conserved site
    1. PS00446 (RNA_POL_D_...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.30.13...
  2. cd07031 (RNAP_II_RPB3)
  3. mobidb-lite (disord...)

Residue annotation

  1. RPB3 - RPB11 heter...
  2. RPB3 - RPB2 intera...
  3. RPB3 - RPB12 inter...
  4. RPB3 - RPB10 inter...
  5. RPB3 - RPB8 intera...
  6. RPB3 - RPB1 intera...

Protein sequence

>MCA_00463_1
MEEDGPKVTIRKITGDEVDFVISDTELYLANAVRRVMIAEIPTIAIDLVEIESNTSVLADEFIAHRLGLIPLDSTYADQL
EYTRDCTCDQYCDKCSVVLTLDARNTSDQTLPIYTRDLQVVRREESNIGRPVFNGPDQRGILICKLRKHQELRLKCIAKK
GIAKEHAKWSPCSAISFEYDPWNKLRHTDYWYEVDPEAEWPKSKNCDWEEAPKENEPFDYNAKPDRFYINVESVGSMKPN
HIVENGIEVLQKKVANIILALDKMEGGLAASGPGGPDDGPGPDGYDRYGQSGYDQGPGGFGGDDEGNDGWGAPDSGAGST
GWF

GO term prediction

Biological Process

GO:0006351 transcription, DNA-templated

Molecular Function

GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0046983 protein dimerization activity

Cellular Component

None predicted.