MCA_00385_1

Protein

Length

287 amino acids

Gene name: ECI1

Description: Peroxisomal delta3,delta2-enoyl-CoA isomerase; hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA

Browser: contigA:1191875-1192739-

RNA-seq: read pairs 814, FPKM 34.9, percentile rank 57.0% (100% = highest expression)

Protein function

Annotation:		ECI1	Peroxisomal delta3,delta2-enoyl-CoA isomerase; hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA
KEGG:	K13239	PECI	peroxisomal 3,2-trans-enoyl-CoA isomerase [EC:5.3.3.8]
EGGNOG:	0PJ85	FG05551.1	peroxisomal D3,D2-enoyl-CoA isomerase
SGD closest match:	S000004274	ECI1	3,2-trans-enoyl-CoA isomerase
CGD closest match:	CAL0000181317	ECI1	Dodecenoyl-CoA isomerase

Protein alignments

	%id	Aln length	E-value
MIA_03563_1	74.91%	271	7e-141	MIA_03563_1
A0A0J9XB23_GEOCN	49.82%	275	2e-83	Similar to Saccharomyces cerevisiae YLR284C ECI1 Peroxisomal delta3,delta2-enoyl-CoA isomerase OS=Geotrichum candidum GN=BN980_GECA07s03772g PE=4 SV=1
A0A060T2D8_BLAAD	49.63%	272	1e-78	ARAD1C24530p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C24530g PE=4 SV=1
UniRef50_A0A060T2D8	49.63%	272	3e-75	ARAD1C24530p n=3 Tax=Saccharomycetales TaxID=4892 RepID=A0A060T2D8_BLAAD
A0A167EN94_9ASCO	49.26%	272	2e-75	Dodecenoyl-CoA isomerase OS=Sugiyamaella lignohabitans GN=ECI1 PE=4 SV=1
Q5A318_CANAL	37.73%	273	1e-49	Dodecenoyl-CoA isomerase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=ECI1 PE=4 SV=1
ECI1_YEAST	34.67%	274	5e-40	3,2-trans-enoyl-CoA isomerase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ECI1 PE=1 SV=1
A0A1E4TFH8_9ASCO	31.99%	272	4e-31	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_106749 PE=4 SV=1
Q6CF43_YARLI	25.48%	208	7e-08	YALI0B10406p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_B10406g PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.2565

Protein family membership

Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type (IPR032259)

Domains and repeats

Domain
4 - 273
ClpP/crotonase-like domain (IPR029045)

1 50 100 150 200 250 287

Detailed signature matches

IPR032259

Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type
1. PF16113 (ECH_2)
  
  14 - 223
  
  Pfam
  PF16113 (ECH_2)
IPR029045

ClpP/crotonase-like domain
1. SSF52096 (ClpP/crot...)
  
  4 - 273
  
  SUPERFAMILY
  SSF52096 (ClpP/crotonase)

no IPR

Unintegrated signatures

Coil

25 - 45

COILS
Coil (Coil)
G3DSA:3.90.22...

4 - 283

GENE3D
G3DSA:3.90.226.10 (G3DSA:3.90.226.10)
cd06558 (crotonase-...)

5 - 226

CDD
cd06558 (crotonase-like)

Residue annotation

substrate binding ...

24F

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)

26S

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)

58K

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)

62A

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)

63G

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)

64A

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)

77D

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)

78L

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)

129P

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)

131I

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)

132G

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)

133L

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)

155P

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)

156F

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)

159L

substrate binding site
24F, 26S, 58K, 62A, 63G, 64A, 77D, 78L, 129P, 131I, 132G, 133L, 155P, 156F, 159L
CDD
cd06558 (crotonase-like)
oxyanion hole (OAH...

64A

oxyanion hole (OAH) forming residues
64A, 133L
CDD
cd06558 (crotonase-like)

133L

oxyanion hole (OAH) forming residues
64A, 133L
CDD
cd06558 (crotonase-like)
trimer interface c...

113H

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

121V

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

142D

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

143F

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

144I

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

145I

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

157A

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

158N

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

159L

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

160G

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

166G

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

168S

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

169Y

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

170T

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

172Q

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

173N

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

178S

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

179L

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

181S

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

182E

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

184L

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

185L

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

188S

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

199G

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

202N

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

223W

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

226D

trimer interface
113H, 121V, 142D, 143F, 144I, 145I, 157A, 158N, 159L, 160G, 166G, 168S, 169Y, 170T, 172Q, 173N, 178S, 179L, 181S, 182E, 184L, 185L, 188S, 199G, 202N, 223W, 226D
CDD
cd06558 (crotonase-like)

Protein sequence

>MCA_00385_1
MPQSITYKVVGKTAIITLNNPKKFNSLSQTEYRRLEQLMQLAEREKDTTITLLQSSGKFFSAGATVDAKIEEADVYDLEY
ITDTTDPKYLTEYLKTRTLYTYSFGARNFTITHTFFNHPKVFVVALNGPVIGLSASIVATADFIIARDSAYMLTPFANLG
LPPEGGTSYTLQNRLGHSLASELLLASSPVKAQALYDVGFINKLVKTSEYQDVDKFNEMVQKWIADKFYNLHDESLVISK
ELMRKPQAPKFLEVEHDEVIAGINMFAQGSPQDRFAKLAAGKLKHKL

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003860 3-hydroxyisobutyryl-CoA hydrolase activity

Cellular Component