MCA_00305_1

Protein

Length

374 amino acids

Gene name: GLY1

Description: Low specificity L-threonine aldolase

Browser: contigA:935496-936621+

RNA-seq: read pairs 5387, FPKM 177.5, percentile rank 86.7% (100% = highest expression)

Protein function

Annotation:		GLY1	Low specificity L-threonine aldolase
KEGG:	K01620	ltaE	threonine aldolase [EC:4.1.2.48]
EGGNOG:	0PGQW	GLY1	Threonine aldolase
SGD closest match:	S000000772	GLY1	Low specificity L-threonine aldolase
CGD closest match:	CAL0000176940	CAALFM_CR04880WA	Threonine aldolase

Protein alignments

	%id	Aln length	E-value
MIA_01655_1	67.66%	368	0.0	MIA_01655_1
A0A0J9YHQ6_GEOCN	62.93%	348	4e-170	Similar to Saccharomyces cerevisiae YEL046C GLY1 Threonine aldolase OS=Geotrichum candidum GN=BN980_GECA01s07963g PE=4 SV=1
UniRef50_A0A0J9YHQ6	62.93%	348	8e-167	Similar to Saccharomyces cerevisiae YEL046C GLY1 Threonine aldolase n=5 Tax=Dikarya TaxID=451864 RepID=A0A0J9YHQ6_GEOCN
A0A060TFV3_BLAAD	58.06%	341	4e-149	ARAD1D17512p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D17512g PE=4 SV=1
Q6CG81_YARLI	55.95%	336	3e-135	YALI0A21417p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_A21417g PE=4 SV=1
A0A161HIB9_9ASCO	53.87%	323	1e-134	Threonine aldolase GLY1 OS=Sugiyamaella lignohabitans GN=GLY1 PE=4 SV=1
A0A1D8PSW2_CANAL	47.77%	358	8e-122	Threonine aldolase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=CAALFM_CR04880WA PE=4 SV=1
GLY1_YEAST	49.12%	342	1e-117	Low specificity L-threonine aldolase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GLY1 PE=1 SV=2
A0A1E3PNS1_9ASCO	45.17%	352	6e-115	Putative low specificity L-threonine aldolase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_81580 PE=4 SV=1
A0A1E4TLT2_9ASCO	51.65%	333	2e-107	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_90966 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1503

Protein family membership

Threonine aldolase (IPR023603)

Domains and repeats

Domain
3 - 251
Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (IPR015421)
252 - 342
Pyridoxal phosphate-dependent transferase, subdomain 2 (IPR015422)
Domain
8 - 340
Pyridoxal phosphate-dependent transferase (IPR015424)
Aromatic amino acid beta-eliminating lyase/threonine aldolase (IPR001597)

1 50 100 150 200 250 300 374

Detailed signature matches

IPR023603

Threonine aldolase
1. PIRSF017617 (Thr_al...)
  
  1 - 354
  
  PIRSF
  PIRSF017617 (Thr_aldolase)
IPR015421

Pyridoxal phosphate-dependent transferase, major region, subdomain 1
1. G3DSA:3.40.64...
  
  3 - 251
  
  GENE3D
  G3DSA:3.40.640.10 (G3DSA:3.40.640.10)
IPR015424

Pyridoxal phosphate-dependent transferase
1. SSF53383 (PLP-depen...)
  
  8 - 340
  
  SUPERFAMILY
  SSF53383 (PLP-dependent transferases)
IPR001597

Aromatic amino acid beta-eliminating lyase/threonine aldolase
1. PF01212 (Beta_elim_...)
  
  9 - 290
  
  Pfam
  PF01212 (Beta_elim_lyase)
IPR015422

Pyridoxal phosphate-dependent transferase, subdomain 2
1. G3DSA:3.90.11...
  
  252 - 342
  
  GENE3D
  G3DSA:3.90.1150.10 (G3DSA:3.90.1150.10)

no IPR

Unintegrated signatures

cd06502 (TA_like)

9 - 337

CDD
cd06502 (TA_like)

Residue annotation

tetramer interface...

10R

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

13T

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

14F

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

15T

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

16V

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

22I

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

25M

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

26T

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

27K

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

61P

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

64T

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

65M

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

72R

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

91Y

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

92R

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

93N

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

94E

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

95A

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

96A

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

97G

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

98I

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

105M

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

106V

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

107Y

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

203S

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

204K

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

210V

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

225I

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

226W

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

229K

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

230Q

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

240M

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

241L

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)

315N

tetramer interface
10R, 13T, 14F, 15T, 16V, 22I, 25M, 26T, 27K, 61P, 64T, 65M, 72R, 91Y, 92R, 93N, 94E, 95A, 96A, 97G, 98I, 105M, 106V, 107Y, 203S, 204K, 210V, 225I, 226W, 229K, 230Q, 240M, 241L, 315N
CDD
cd06502 (TA_like)
pyridoxal 5'-phosp...

63G

pyridoxal 5'-phosphate binding site
63G, 64T, 67N, 143E, 173D, 176R, 201C, 204K
CDD
cd06502 (TA_like)

64T

pyridoxal 5'-phosphate binding site
63G, 64T, 67N, 143E, 173D, 176R, 201C, 204K
CDD
cd06502 (TA_like)

67N

pyridoxal 5'-phosphate binding site
63G, 64T, 67N, 143E, 173D, 176R, 201C, 204K
CDD
cd06502 (TA_like)

143E

pyridoxal 5'-phosphate binding site
63G, 64T, 67N, 143E, 173D, 176R, 201C, 204K
CDD
cd06502 (TA_like)

173D

pyridoxal 5'-phosphate binding site
63G, 64T, 67N, 143E, 173D, 176R, 201C, 204K
CDD
cd06502 (TA_like)

176R

pyridoxal 5'-phosphate binding site
63G, 64T, 67N, 143E, 173D, 176R, 201C, 204K
CDD
cd06502 (TA_like)

201C

pyridoxal 5'-phosphate binding site
63G, 64T, 67N, 143E, 173D, 176R, 201C, 204K
CDD
cd06502 (TA_like)

204K

pyridoxal 5'-phosphate binding site
63G, 64T, 67N, 143E, 173D, 176R, 201C, 204K
CDD
cd06502 (TA_like)
catalytic residue ...

204K

catalytic residue
204K
CDD
cd06502 (TA_like)

Protein sequence

>MCA_00305_1
MTPRSAAEFRSDTFTVPTREMIEAMTKASMGDDVYVEDEDTNSFQDRVCKLAGKEAAAFMPSGTMSNQVGIRVNLFQPPY
SIVADDRAHVYRNEAAGIAILSQAMVYPVNPSNGKYITLEDIKERIIDFDDIHVPPTKLICLENTISGIVVPLKEIARIS
DYARSHGIRMHLDGARLWNASAESGVSIEEYCRYFDTVSLCFSKGMGAPVGSVLVGDKKSIDRAIWIRKQQGGGMRQIGM
LAAAANISIDKVWPTMKATHDMVKQVAKWMAEELGIKFEVPVETNFIFIDAKKSGLDISLLEKAAAERGLNVLSNRITFH
HQISEEAVELLKEATKEAIEQSRKAGIKPVEEDANSGFAVSKSGGVHVYAPGPQ

GO term prediction

Biological Process

GO:0006520 cellular amino acid metabolic process

Molecular Function

GO:0003824 catalytic activity
GO:0016829 lyase activity

Cellular Component

None predicted.

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Entry type

Status

Per-residue features