MCA_00151_1

Protein

Length

1,662 amino acids

Description: Retrotransposon Ty3/Gypsy Gag-Pol polyprotein

RNA-seq: read pairs 917, FPKM 6.8, percentile rank 20.3% (100% = highest expression)

Protein function

Annotation:			Retrotransposon Ty3/Gypsy Gag-Pol polyprotein
EGGNOG:	0PGPZ		to reverse transcriptase
SGD closest match:	S000007347	TY3B-G	Transposon Ty3-G Gag-Pol polyprotein
CGD closest match:	CAL0000191508	ORF298	Uncharacterized protein

Protein alignments

	%id	Aln length	E-value
A0A060T683_BLAAD	42.14%	1139	0.0	ARAD1B13926p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B13926g PE=4 SV=1
UniRef50_A0A060T683	42.14%	1139	0.0	ARAD1B13926p n=1 Tax=Blastobotrys adeninivorans TaxID=409370 RepID=A0A060T683_BLAAD
B5FVH8_YARLI	34.94%	1119	0.0	YALI0E14388p2 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E14388g2 PE=4 SV=1
YG31B_YEAST	39.07%	942	0.0	Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TY3B-G PE=1 SV=3
A0A167D9C8_9ASCO	33.04%	905	2e-134	Gag-pol fusion protein OS=Sugiyamaella lignohabitans GN=AWJ20_901 PE=4 SV=1
A0A1D8PI28_CANAL	24.97%	781	1e-57	Uncharacterized protein OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=ORF298 PE=4 SV=1
A0A0J9XCS2_GEOCN	33.85%	130	4e-09	Uncharacterized protein OS=Geotrichum candidum GN=BN980_GECA10s00604g PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0539

Protein family membership

None predicted.

Domains and repeats

Domain
504 - 614
Aspartic peptidase domain (IPR021109)
731 - 910
Reverse transcriptase domain (IPR000477)
1,258 - 1,483
Ribonuclease H-like domain (IPR012337)
Integrase, catalytic core (IPR001584)

1 200 400 600 800 1000 1200 1400 1662

Detailed signature matches

IPR021109

Aspartic peptidase domain
1. G3DSA:2.40.70.10
  
  504 - 614
  
  GENE3D
  G3DSA:2.40.70.10 (G3DSA:2.40.70.10)
IPR000477

Reverse transcriptase domain
1. PF00078 (RVT_1)
  
  750 - 910
  
  Pfam
  PF00078 (RVT_1)
2. PS50878 (RT_POL)
  
  731 - 910
  
  PROSITE profiles
  PS50878 (RT_POL)
IPR012337

Ribonuclease H-like domain
1. SSF53098 (Ribonucle...)
  
  1,263 - 1,418
  
  SUPERFAMILY
  SSF53098 (Ribonuclease H-like)
2. G3DSA:3.30.42...
  
  1,258 - 1,483
  
  GENE3D
  G3DSA:3.30.420.10 (G3DSA:3.30.420.10)
IPR001584

Integrase, catalytic core
1. PS50994 (INTEGRASE)
  
  1,262 - 1,433
  
  PROSITE profiles
  PS50994 (INTEGRASE)

no IPR

Unintegrated signatures

G3DSA:3.10.10.10

698 - 830

GENE3D
G3DSA:3.10.10.10 (G3DSA:3.10.10.10)
G3DSA:3.30.70...

914 - 1,041

GENE3D
G3DSA:3.30.70.270 (G3DSA:3.30.70.270)

831 - 913

GENE3D
G3DSA:3.30.70.270 (G3DSA:3.30.70.270)
SSF56672 (DNA/RNA p...)

676 - 1,107

SUPERFAMILY
SSF56672 (DNA/RNA polymerases)
cd00303 (retropepsi...)

528 - 606

CDD
cd00303 (retropepsin_like)
cd01647 (RT_LTR)

734 - 909

CDD
cd01647 (RT_LTR)
cd09274 (RNase_HI_R...)

1,002 - 1,123

CDD
cd09274 (RNase_HI_RT_Ty3)
mobidb-lite (disord...)

237 - 273

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

Catalytic residue ...

531D

Catalytic residue
531D
CDD
cd00303 (retropepsin_like)
catalytic motif cd...

531D

catalytic motif
531D, 532S, 533G
CDD
cd00303 (retropepsin_like)

532S

catalytic motif
531D, 532S, 533G
CDD
cd00303 (retropepsin_like)

533G

catalytic motif
531D, 532S, 533G
CDD
cd00303 (retropepsin_like)
inhibitor binding ...

531D

inhibitor binding site
531D, 533G, 535A, 561L, 562S, 563S, 601I
CDD
cd00303 (retropepsin_like)

533G

inhibitor binding site
531D, 533G, 535A, 561L, 562S, 563S, 601I
CDD
cd00303 (retropepsin_like)

535A

inhibitor binding site
531D, 533G, 535A, 561L, 562S, 563S, 601I
CDD
cd00303 (retropepsin_like)

561L

inhibitor binding site
531D, 533G, 535A, 561L, 562S, 563S, 601I
CDD
cd00303 (retropepsin_like)

562S

inhibitor binding site
531D, 533G, 535A, 561L, 562S, 563S, 601I
CDD
cd00303 (retropepsin_like)

563S

inhibitor binding site
531D, 533G, 535A, 561L, 562S, 563S, 601I
CDD
cd00303 (retropepsin_like)

601I

inhibitor binding site
531D, 533G, 535A, 561L, 562S, 563S, 601I
CDD
cd00303 (retropepsin_like)
Active site flap c...

561L

Active site flap
561L, 562S, 563S, 564A, 568S, 569Q, 570T, 571C
CDD
cd00303 (retropepsin_like)

562S

Active site flap
561L, 562S, 563S, 564A, 568S, 569Q, 570T, 571C
CDD
cd00303 (retropepsin_like)

563S

Active site flap
561L, 562S, 563S, 564A, 568S, 569Q, 570T, 571C
CDD
cd00303 (retropepsin_like)

564A

Active site flap
561L, 562S, 563S, 564A, 568S, 569Q, 570T, 571C
CDD
cd00303 (retropepsin_like)

568S

Active site flap
561L, 562S, 563S, 564A, 568S, 569Q, 570T, 571C
CDD
cd00303 (retropepsin_like)

569Q

Active site flap
561L, 562S, 563S, 564A, 568S, 569Q, 570T, 571C
CDD
cd00303 (retropepsin_like)

570T

Active site flap
561L, 562S, 563S, 564A, 568S, 569Q, 570T, 571C
CDD
cd00303 (retropepsin_like)

571C

Active site flap
561L, 562S, 563S, 564A, 568S, 569Q, 570T, 571C
CDD
cd00303 (retropepsin_like)
putative NTP bindi...

797D

putative NTP binding site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 861D
CDD
cd01647 (RT_LTR)

798L

putative NTP binding site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 861D
CDD
cd01647 (RT_LTR)

799R

putative NTP binding site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 861D
CDD
cd01647 (RT_LTR)

800S

putative NTP binding site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 861D
CDD
cd01647 (RT_LTR)

801G

putative NTP binding site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 861D
CDD
cd01647 (RT_LTR)

802Y

putative NTP binding site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 861D
CDD
cd01647 (RT_LTR)

831F

putative NTP binding site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 861D
CDD
cd01647 (RT_LTR)

861D

putative NTP binding site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 861D
CDD
cd01647 (RT_LTR)
putative active si...

797D

putative active site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 832G, 859Y, 861D, 862D, 906L, 907G
CDD
cd01647 (RT_LTR)

798L

putative active site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 832G, 859Y, 861D, 862D, 906L, 907G
CDD
cd01647 (RT_LTR)

799R

putative active site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 832G, 859Y, 861D, 862D, 906L, 907G
CDD
cd01647 (RT_LTR)

800S

putative active site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 832G, 859Y, 861D, 862D, 906L, 907G
CDD
cd01647 (RT_LTR)

801G

putative active site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 832G, 859Y, 861D, 862D, 906L, 907G
CDD
cd01647 (RT_LTR)

802Y

putative active site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 832G, 859Y, 861D, 862D, 906L, 907G
CDD
cd01647 (RT_LTR)

831F

putative active site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 832G, 859Y, 861D, 862D, 906L, 907G
CDD
cd01647 (RT_LTR)

832G

putative active site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 832G, 859Y, 861D, 862D, 906L, 907G
CDD
cd01647 (RT_LTR)

859Y

putative active site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 832G, 859Y, 861D, 862D, 906L, 907G
CDD
cd01647 (RT_LTR)

861D

putative active site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 832G, 859Y, 861D, 862D, 906L, 907G
CDD
cd01647 (RT_LTR)

862D

putative active site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 832G, 859Y, 861D, 862D, 906L, 907G
CDD
cd01647 (RT_LTR)

906L

putative active site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 832G, 859Y, 861D, 862D, 906L, 907G
CDD
cd01647 (RT_LTR)

907G

putative active site
797D, 798L, 799R, 800S, 801G, 802Y, 831F, 832G, 859Y, 861D, 862D, 906L, 907G
CDD
cd01647 (RT_LTR)
putative nucleic a...

832G

putative nucleic acid binding site
832G
CDD
cd01647 (RT_LTR)
active site cd09274

1,006D

active site
1,006D, 1,048E, 1,073D, 1,117D
CDD
cd09274 (RNase_HI_RT_Ty3)

1,048E

active site
1,006D, 1,048E, 1,073D, 1,117D
CDD
cd09274 (RNase_HI_RT_Ty3)

1,073D

active site
1,006D, 1,048E, 1,073D, 1,117D
CDD
cd09274 (RNase_HI_RT_Ty3)

1,117D

active site
1,006D, 1,048E, 1,073D, 1,117D
CDD
cd09274 (RNase_HI_RT_Ty3)
RNA/DNA hybrid bin...

1,006D

RNA/DNA hybrid binding site
1,006D, 1,007A, 1,008S, 1,009N, 1,043A, 1,044V, 1,045R, 1,048E, 1,073D, 1,107Y, 1,121R
CDD
cd09274 (RNase_HI_RT_Ty3)

1,007A

RNA/DNA hybrid binding site
1,006D, 1,007A, 1,008S, 1,009N, 1,043A, 1,044V, 1,045R, 1,048E, 1,073D, 1,107Y, 1,121R
CDD
cd09274 (RNase_HI_RT_Ty3)

1,008S

RNA/DNA hybrid binding site
1,006D, 1,007A, 1,008S, 1,009N, 1,043A, 1,044V, 1,045R, 1,048E, 1,073D, 1,107Y, 1,121R
CDD
cd09274 (RNase_HI_RT_Ty3)

1,009N

RNA/DNA hybrid binding site
1,006D, 1,007A, 1,008S, 1,009N, 1,043A, 1,044V, 1,045R, 1,048E, 1,073D, 1,107Y, 1,121R
CDD
cd09274 (RNase_HI_RT_Ty3)

1,043A

RNA/DNA hybrid binding site
1,006D, 1,007A, 1,008S, 1,009N, 1,043A, 1,044V, 1,045R, 1,048E, 1,073D, 1,107Y, 1,121R
CDD
cd09274 (RNase_HI_RT_Ty3)

1,044V

RNA/DNA hybrid binding site
1,006D, 1,007A, 1,008S, 1,009N, 1,043A, 1,044V, 1,045R, 1,048E, 1,073D, 1,107Y, 1,121R
CDD
cd09274 (RNase_HI_RT_Ty3)

1,045R

RNA/DNA hybrid binding site
1,006D, 1,007A, 1,008S, 1,009N, 1,043A, 1,044V, 1,045R, 1,048E, 1,073D, 1,107Y, 1,121R
CDD
cd09274 (RNase_HI_RT_Ty3)

1,048E

RNA/DNA hybrid binding site
1,006D, 1,007A, 1,008S, 1,009N, 1,043A, 1,044V, 1,045R, 1,048E, 1,073D, 1,107Y, 1,121R
CDD
cd09274 (RNase_HI_RT_Ty3)

1,073D

RNA/DNA hybrid binding site
1,006D, 1,007A, 1,008S, 1,009N, 1,043A, 1,044V, 1,045R, 1,048E, 1,073D, 1,107Y, 1,121R
CDD
cd09274 (RNase_HI_RT_Ty3)

1,107Y

RNA/DNA hybrid binding site
1,006D, 1,007A, 1,008S, 1,009N, 1,043A, 1,044V, 1,045R, 1,048E, 1,073D, 1,107Y, 1,121R
CDD
cd09274 (RNase_HI_RT_Ty3)

1,121R

RNA/DNA hybrid binding site
1,006D, 1,007A, 1,008S, 1,009N, 1,043A, 1,044V, 1,045R, 1,048E, 1,073D, 1,107Y, 1,121R
CDD
cd09274 (RNase_HI_RT_Ty3)

Protein sequence

>MCA_00151_1
MSSSIKNENPTTHTTEQYNSIIIECEKVPRIAKITAYKGQSDPSIINEWISKIESAMRTRFVDSRYWASAAFDLALFDGP
AEKWAKDGLNVVRANNGPIAPLIEWKTFCDAMRVFFVPEAVERALEAKVYRMQKSDTGSVIQYVADLRNCLYLLKPEVRR
KINVKQIFFHGLDSEMRDKLEVFYDNLSSDDLMQRALRMCHEEGRWKFRHFFDPNQTEMTLVDPLAIKAVQASPEPTVGV
DNINYNRNNSKNRNNKGSDNGKKNNNQRNAAGFPANISLPKQEEECFFELQFQSKKLRKPTLKADVGSINKALSVHPPEL
VPTPVPDPDPDPDPEGFIDPCNRFSCLPLDEAIDDDTYEGHNMCCNPIMMDVPRPGEEAPLSLDAGEGDHTRQTLNNNSV
QIARSSDVDAVWRRVANTGGECTKGPNSVPPMKNCSLNFLVDVEEVTDKNLNTKPQLVGKGPPTPKFSVKVSARPIVPPV
TPPISEMEISKIDINAVTPDDGPEIGKKIVVPTCIKLQSRDWKPRFNALIDSGAASYFVSHEAIQRNHLEHLTVPCPPIK
LSSAFSNSQTCKRKVQLTFHIGPIEATKSFFVVHGLSHDMILGTPFTAEFADFVDLKSFSVAGVGSVADDPLFVSAIEFE
RLAKSKENAIGICVLKFDEPVKEPASLEVLPFDASEYKDVLTNDPPTGLPPERDVTHPIDVVPGSTPPYRSYYRLSKAES
DYLTEELSKMANTGMIRPSSSPYSAPVLFVKKKDGSLRLCVDYRMLNNITIKNRYPLPFIEEMLNKVEDARVFSKLDLRS
GYHQIRIRPQDIEKTAFSTSTGHWEFLVMPFGLTNAPATFQNFMNDIFRPYIDKFVNVYLDDILVFSKTKEEHVQHLKKV
LDVLKENKLVANLKKCEFFKTELDFLGYKLDAKGIHITDEKVRAVKEFPIPKTVKECQRFLGMTNYYRKFVPNFSGIAAP
LHDFTSEKTKWTETQTQAFETLKEKLIDAPILISPNSKHTYVLTTDASNKNIGATLEQYEGSRLLGVIAYFSRKLKPTEV
NYAVRDLEFLAVVDALQHFRPLLLGRHFILRTDHFSLTQLQSQTKPPHGRIARWFDALADYDFTTQYIKGKQNVVADALS
RQPSVNAIDTNDATNEEEELMSTPSDDIIRRVRDKLDSDDYFANVIRILNDENDPAHRTISSKYHLDDGLLYFKITPTVP
YEPTLLLWLTLMVIMDHTAPFLRLAHFYYWPVMFPEVKRIVDHCPDCAYTQPNKKTHGYLQPLQIPQQRWDSISLDFVSG
LPEVDGYDMILVVVDRLSKRARFLPTTKTLTGKDCAYLLHREIFKIHGFPLDIVSDRDVRFQELFWKTMHLINGTKNLQS
TSFHPETDGQTERVNRVLRALMSSHAVQYGFNWPFHLATLEFSYNSTFQATIRASPFMADLGRVPRNPSFITPPMSFTGK
IEAEDFAIITQAILARTRDFIAEAQDKQSLYANKTRTALVLKENDEVLVRYAYSLAPNSNKYIDSVNLFTGPYRVVKKID
DNVYEIDLPEYDRSRRNINVSRLKKFEPSEAFPRSPPLNLEAVKARITEISGIVGETDDAYLLQFSNCRPGHIVAVNKQV
LNLLSPAEQERIKRNANAMTRNLMTHPDLAKEQFTHNQHLWRNQALSFFNQEQINHMNRDDS

GO term prediction

Biological Process

GO:0015074 DNA integration

Molecular Function

GO:0003676 nucleic acid binding

Cellular Component